Expression of Ang-1, Ang-2 and Tie-2 and Their Roles in Angiogenesis in Endometrial Adenocarcinoma

doi:10.3877/cma.j.issn.1673-5250.2013.04.007

Objective

To study the expression of angiopoietin(Ang)-1, Ang-2 and their receptor endothelium-specific receptor tyrosine kinase (Tie)-2 in endometrial adenocarcinoma.

Methods

From May 2004 to 2012, 92 cases of human primary endometrial carcinoma tissue, 54 cases of atypical hyperplasia tissue and 43 cases of normal proliferative endometrium were included in the study, and divided into endometrial carcinoma group(n=92), atypical hyperplasia tissue group(n=54) and normal proliferative endometrium group(n=43). The expression of Ang-1, Ang-2 and Tie-2 were detected among three groups. MVD value was calculated among three groups as well. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Central Hospital of Panyu. There were no significant differences in general clinical data of tissue-taken patients among three groups.

Results

①The positive rate of Ang-2 and Tie-2 in endometrial carcinoma group were significantly higher than those of atypical hyperplasia tissue group and normal proliferative endometrium group, meanwhile the positive rate of Ang-1 were significantly lower than other two groups(P<0.05). ②There had significant differences in MVD value between endometrial carcinoma group and atypical hyperplasia tissue group or normal proliferative endometrium group respectively(P<0.05). ③There was a positive correlation between expression of Ang-2, Tie-2 and MVD value (P<0.05), however, the expression of Ang-1 was negatively correlated with MVD value (P<0.05). ④ The expression of Ang-2 and Tie-2 were increased as the International Federation of Gynecology and Obstetrics (FIGO) stages and myometrial invasion degrees increased (P<0.05).

Conclusions

Ang-1, Ang-2 and Tie-2 play important roles in the angiogenesis of endometrial carcinoma, and have strong relationship with FIGO stages and myometrial invasion degrees in endometrial carcinoma.

Key words: endometrial adenocarcinoma, angiopoietins, microvessel density

表1 3组Ang-1，Ang -2和Tie-2阳性表达率比较[n(%)]

Table 1 Comparison of positive expression rate of Ang-1，Ang -2 and Tie-2 among three groups[n(%)]

组别	n	阳性表达率
组别	n	Ang-1	Ang-2	Tie-2
子宫内膜腺癌组	92	28(30.4)	70(76.1)	56(60.8)
非典型增生内膜组	54	30(55.6)^c	24(44.4)^a	18(33.3)^a
增生期子宫内膜组	43	28(65.1)^cd	15(34.9)^bd	14(32.6)^bd

表1 3组Ang-1，Ang -2和Tie-2阳性表达率比较[n(%)]

Table 1 Comparison of positive expression rate of Ang-1，Ang -2 and Tie-2 among three groups[n(%)]

组别	n	阳性表达率
组别	n	Ang-1	Ang-2	Tie-2
子宫内膜腺癌组	92	28(30.4)	70(76.1)	56(60.8)
非典型增生内膜组	54	30(55.6)^c	24(44.4)^a	18(33.3)^a
增生期子宫内膜组	43	28(65.1)^cd	15(34.9)^bd	14(32.6)^bd

图1 Ang-1，Ang -2和Tie-2在子宫内膜癌肿瘤细胞细胞质中表达

Figure 1 Expression of Ang-1，Ang-2 and Tie-2 in endometrial adenocarcinoma

表2 3组MVD值比较(±s)

Table 2 Comparison of MVD value among three groups(±s)

组织类型	n	MVD值(个)
子宫内膜腺癌组	92	28.9±6.3
非典型增生内膜组	54	19.2±5.2^a
增生期子宫内膜组	43	11.3±4.7^b

表2 3组MVD值比较(±s)

Table 2 Comparison of MVD value among three groups(±s)

组织类型	n	MVD值(个)
子宫内膜腺癌组	92	28.9±6.3
非典型增生内膜组	54	19.2±5.2^a
增生期子宫内膜组	43	11.3±4.7^b

表3 子宫内膜腺癌组中Ang-1，Ang -2和Tie-2表达与MVD的关系[n(%)]

Table 3 Relationship between expression of Ang-1，Ang-2 and Tie-2 and MVD in endometrial adenocarcinoma tissue [n(%)]

分组	n	Ang-1		Ang-2		Tie-2
分组	n	－	＋	－	＋	－	＋
高MVD组	55	33(60.0)	22(40.0)	19(34.5)	36(65.5)	22(40.0)	33(60.0)
低MVD组	37	31(83.8)	6(16.2)	3(8.1)	34(91.9)	14(37.8)	23(62.2)

表3 子宫内膜腺癌组中Ang-1，Ang -2和Tie-2表达与MVD的关系[n(%)]

Table 3 Relationship between expression of Ang-1，Ang-2 and Tie-2 and MVD in endometrial adenocarcinoma tissue [n(%)]

分组	n	Ang-1		Ang-2		Tie-2
分组	n	－	＋	－	＋	－	＋
高MVD组	55	33(60.0)	22(40.0)	19(34.5)	36(65.5)	22(40.0)	33(60.0)
低MVD组	37	31(83.8)	6(16.2)	3(8.1)	34(91.9)	14(37.8)	23(62.2)

表4 Ang-2和Tie-2表达阳性率与子宫内膜癌临床病理特征的关系[n(%)]

Table 4 Relationship between expression of Ang-2，Tie-2 and clinicopathologic features of endometrial adenocarcinoma tissue [n(%)]

分期		n	Ang-2阳性表达率	Tie-2阳性表达率
FIGO分期		92	?	?
?	Ⅰ期	49	23(46.9)	20(40.8)
?	Ⅱ期	20	16(80.0)	17(85.0)
?	Ⅲ期	23	18(78.3)	19(82.6)
组织病理学分期		92	?	?
?	G₁	42	29(69.0)	25(59.5)
?	G₂	23	15(65.2)	14(60.9)
?	G₃	27	13(48.1)	14(51.9)
肌层浸润程度		92	?	?
?	无	12	3(25.0)	5(41.7)
?	<1/2	26	19(73.1)	16(61.5)
?	≥1/2	54	49(90.7)	43(79.6)

表4 Ang-2和Tie-2表达阳性率与子宫内膜癌临床病理特征的关系[n(%)]

Table 4 Relationship between expression of Ang-2，Tie-2 and clinicopathologic features of endometrial adenocarcinoma tissue [n(%)]

分期		n	Ang-2阳性表达率	Tie-2阳性表达率
FIGO分期		92	?	?
?	Ⅰ期	49	23(46.9)	20(40.8)
?	Ⅱ期	20	16(80.0)	17(85.0)
?	Ⅲ期	23	18(78.3)	19(82.6)
组织病理学分期		92	?	?
?	G₁	42	29(69.0)	25(59.5)
?	G₂	23	15(65.2)	14(60.9)
?	G₃	27	13(48.1)	14(51.9)
肌层浸润程度		92	?	?
?	无	12	3(25.0)	5(41.7)
?	<1/2	26	19(73.1)	16(61.5)
?	≥1/2	54	49(90.7)	43(79.6)

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