Expression and Clinical Significance of Leptin in Cervical Leison

doi:10.3877/cma.j.issn.1673-5250.2012.04.018

Objective

To evaluate the expression and clinical significance of leptin in cervical leison.

Methods

A totol of 142 women with cervical lesion were recruited in Tongji Hospital from September 2003 to December 2004.They were divided into chronic cervicitis group(n=12), cervical intraepithelial neoplasia(CIN)Ⅰgroup(n=23), CINⅡgroup(n=25), CINⅢgroup(n=21)and cervical cancer group(n=58) according to the histopathological results.Microvessels were immunohistochemical lablled with an antibody to CD₃₄. Microscope analysis was used to evaluate microvessel density (MVD). The study protocal was approved by the Ethical Review Board of Investigation in Human Being of Xiangan Hospital. Informed consent was obtained from each patients.

Results

The expression of leptin was significantly higher in CIN and cervical cancer groups than that in chronic cervicitis group(P<0.05). In addition, in cervical cancer group, there was no significant difference for leptin and CD₃₄ expression in different age and clinical stages(P>0.05), however, they were positively correlation with the degree of malignance and distant metastasis(P<0.05).

Conclusions

Leptin is an important vascular endothelial growth factor(VEGF) in cervical cancer and local overexpression of leptin in cervical cancer may contribute to tumor progression by increasing angiogenesis and tumor growth.

Key words: leptin, CD₃₄, microvessel density, cervical cancer, cervical intraepithelial neoplasia

表1 4组瘦素和CD₃₄表达情况比较[n(%)]

Table1 Comparison of expression of leptin and CD₃₄ among four groups[n(%)]

组别	n	瘦素表达					MVD值(/HP，±s)
组别	n	－	＋	＋＋	＋＋＋	阳性表达率	MVD值(/HP，±s)
宫颈炎组	15	11	4	0	0	4(26.7)	14.93±6.95
CINⅠ组	23	12	7	3	1	11(47.8)	16.12±7.35
CINⅡ组	25	10	9	3	3	15(60.0)	17.25±1.27
CINⅢ组	21	4	6	7	4	17(81.0)	19.38±6.74
宫颈癌组	58	2	10	21	25	56(96.6)	22.51±3.09

表1 4组瘦素和CD₃₄表达情况比较[n(%)]

Table1 Comparison of expression of leptin and CD₃₄ among four groups[n(%)]

组别	n	瘦素表达					MVD值(/HP，±s)
组别	n	－	＋	＋＋	＋＋＋	阳性表达率	MVD值(/HP，±s)
宫颈炎组	15	11	4	0	0	4(26.7)	14.93±6.95
CINⅠ组	23	12	7	3	1	11(47.8)	16.12±7.35
CINⅡ组	25	10	9	3	3	15(60.0)	17.25±1.27
CINⅢ组	21	4	6	7	4	17(81.0)	19.38±6.74
宫颈癌组	58	2	10	21	25	56(96.6)	22.51±3.09

图1 瘦素在宫颈炎组中的表达(×200，SP)

Figure 1 Leptin expression in cervicitis group(×200，SP)

图2 瘦素在CINⅠ组中的表达(×200，SP)

Figure 2 Leptin expression in CINⅠgroup(×200，SP)

图3 瘦素在CINⅡ组中的表达(×200，SP)

Figure 3 Leptin expression in CINⅡgroup(×200，SP)

图4 瘦素在CINⅢ组中的表达(×200，SP)

Figure 4 Leptin expression in CINⅢ group(×200，SP)

图5 瘦素在宫颈癌组中的表达(×200，SP)

Figure 5 Leptin expression in cervical cancer group(×200，SP)

图6 CD₃₄在宫颈癌组中的表达(×200，SP)

Figure 6 CD₃₄ expression in cervical cancer group(×200，SP)

表2 瘦素和CD₃₄表达的多因素logistic回归分析

Table 2 Multiple－factor logistic regression analysis of expression of leptin and CD₃₄

自变量	r	s	Wald值	P	OR	95%CI
自变量	r	s	Wald值	P	OR	Lower	Upper
年龄	0.263	0.633	0.570	0.676	1.153	0.374	4.527
分化程度	1.612	0.552	8.125	0.008	5.056	1.716	15.088
临床分期	0.606	0.471	1.103	0.058	1.735	0.749	4.621
淋巴结转移	3.849	0.714	18.742	0.001	14.352	5.307	28.292

表2 瘦素和CD₃₄表达的多因素logistic回归分析

Table 2 Multiple－factor logistic regression analysis of expression of leptin and CD₃₄

自变量	r	s	Wald值	P	OR	95%CI
自变量	r	s	Wald值	P	OR	Lower	Upper
年龄	0.263	0.633	0.570	0.676	1.153	0.374	4.527
分化程度	1.612	0.552	8.125	0.008	5.056	1.716	15.088
临床分期	0.606	0.471	1.103	0.058	1.735	0.749	4.621
淋巴结转移	3.849	0.714	18.742	0.001	14.352	5.307	28.292

[1]	Liu HL, Yang Y, Gao FY, et al.Expression and clinical significance of ABCG2 and CD₁₃₃ protein in cervical carcinoma[J]. Prog Obstet Gynecol, 2010, 19(2):115-118.
[2]	Weidner N. Intratumor microvessel density as a prognostic factor in cancer[J]. Am J Pathol, 1995, 147(1):9-19.
[3]	Cava AL, Alviggi C, Matarese G. Unraveling the multiple roles of leptin in inflammation and autoimmunity[J]. J Mol Med, 2004, 82:4-11.
[4]	Ruben RG, Amber W, Yanho X, et al. Leptin-signaling inhibition results in efficient anti-tumor activity in estrogen receptor positive or negative breast cancer[J]. Breast Cancer Res, 2009, 11(3):36-48.
[5]	Yin N, Wang D, Zhang H, et al. Molecular mechanisms involved in the growth stimulation of breast cancer cells by leptin[J]. Cancer Res, 2004, 64(16):5870-5875.
[6]	Hee SK. Leptin and leptin receptor expression in breast cancer[J]. Cancer Res Treat, 2009, 41(3):155-163.
[7]	Komal R, Heping Y, Meng X, et al. Leptin's mitogenic effect in human liver cancer cells requires induction of both methionine adenosyltransferase 2A and 2β[J].Hepatology, 2008, 47(2):521-531.
[8]	Giammarco F, Gianfranco A, Chiara R, et al. Leptin enhances cholangiocarcinoma cell growth[J]. Cancer Res, 2008, 68(16):6752-6761.
[9]	Vieira SC, Silva BB, Pinto GA, et al. CD₃₄ as a marker for evaluating angiogenesis in cervical cancer[J]. Pathol Res Pract, 2005, 201(4):313-318.
[10]	Suganami E, Takagi H, Ohashi H, et al.Leptin stimulates ischemia-induced retinal neovascularization: Possible role of vascular endothelial growth factor expressed in retinal endothelial cells[J]. Diabetes, 2004, 53(9):2443-2448.

[1]	Mei Tang, Fan Yang. Current research status between breastfeeding and childhood simple obesity[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2022, 18(03): 269-274.
[2]	Yiran Wang, Ping Wang. Interpretation of the Guidelines of the Brazilian Society of Oncologic Surgery for Pelvic Exenteration in the Treatment of Cervical Cancer[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2021, 17(02): 142-151.
[3]	Xiaofei Sun, Yiqun Gu, Aichun Wang, Li Wang, Fanfan Meng, Jun Wang, lijuan Lu. Evaluation on cervical intraepithelial neoplasia for patients with cervicitis by p16/Ki-67 double staining[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2022, 16(06): 418-425.
[4]	Yingying Wang, Kana Wang, Sijing Chen, Na Wang, Ying Zheng. Laparoscopic radical trachelectomy for early cervical cancer[J]. Chinese Journal of Laparoscopic Surgery(Electronic Edition), 2022, 15(06): 372-375.
[5]	Cankun Zhou, Qingdong Li, Zhuofei Jiang, Lijie Zhao, Yuhua Zheng, Xiaochun Liu, Qinghuang Xie, Xiaobin Huang. Vaginal radical hysterectomy combined with transvaginal endoscopic extraperitoneal lymph node dissection[J]. Chinese Journal of Laparoscopic Surgery(Electronic Edition), 2022, 15(06): 368-371.
[6]	Yunyue Liu, Wensheng Fan, Chenglei Gu, Xin Ma, Hongyi Liu, Yuanguang Meng. Da Vinci robot MDT mode total pelvic exenteration in the treatment of advanced cervical cancer: a case report[J]. Chinese Journal of Laparoscopic Surgery(Electronic Edition), 2022, 15(04): 249-251.
[7]	Ting Zhang, Shanshan Qin, Shengsheng Yu, Juanpeng Yu, Yuchen Song, Yingchun Gao. A novel direct approach to the deep uterine vein in laparoscopic radical hysterectomy[J]. Chinese Journal of Laparoscopic Surgery(Electronic Edition), 2021, 14(06): 363-364.
[8]	Yingjie Su, Lei Dou, Dongli Tian, Enting Lu, Yi Zhang. The situs inversus totalis (SIT) patient undergoing robot assisted radical hysterectomy surgery successfully: a case report[J]. Chinese Journal of Laparoscopic Surgery(Electronic Edition), 2021, 14(04): 245-247.
[9]	Yuying Pan, Li Sun, Guixiang Wang, Hua Li, Meng Yang. A case of recurrent cervical cancer treated by laparoscopic operation[J]. Chinese Journal of Laparoscopic Surgery(Electronic Edition), 2021, 14(01): 53-57.
[10]	Jing Guo, Weihong Qi. Effects of circ_0000218 on the proliferation, migration and invasion of cervical cancer HeLa cells by sponging miR-1182[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2021, 11(02): 113-119.
[11]	Hui Fang, Fei Li, Fan Zhang, Qiang Wei, Qiangpu Chen. Effect of exogenous leptin on intestinal mucosal proliferation in rats with obstructive jaundice[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(05): 575-580.
[12]	Siyu Yang, Jingjing Yang, Ping Zhang, Qiao Liu, Jie Wu, Xiangjin Huang, Yijie Wang, Jingyun Fu. Leptin modulates α1-adrenergic receptor-mediated CaMKK-AMPKα signaling in GT1-7 cells[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(05): 569-574.
[13]	Mi Li, huajuan Qiu, Yanqin Ji, Minghui Zhou. Effect of P16 and Ki67 expression and viral load detection on outcome of cervical lesions in woman of childbearing age with cervical intraepithelial neoplasia grade Ⅱ complicated with high-risk human papillomavirus infection[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(03): 272-278.
[14]	Hongjian Duan, Jianhao Zhang, Xinwei Han, Yanping Zhao, Yuhong Hou, Jie Yang, Su Yan, Shengdong Qin. Analysis of individualized interventional treatment of vesicovaginal fistula[J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2022, 10(01): 45-49.
[15]	Man Yin, Linqing Yang, Yunfei Wang. Diagnostic characteristics of synchronous mucinous metaplasia and neoplasia of the female genital tract and literature review[J]. Chinese Journal of Diagnostics(Electronic Edition), 2022, 10(03): 193-196.

Viewed

Full text

Abstract

Please choose a citation manager

Content to export