Expression and Clinical Significance of Leptin in Cervical Leison

doi:10.3877/cma.j.issn.1673-5250.2012.04.018

Objective

To evaluate the expression and clinical significance of leptin in cervical leison.

Methods

A totol of 142 women with cervical lesion were recruited in Tongji Hospital from September 2003 to December 2004.They were divided into chronic cervicitis group(n=12), cervical intraepithelial neoplasia(CIN)Ⅰgroup(n=23), CINⅡgroup(n=25), CINⅢgroup(n=21)and cervical cancer group(n=58) according to the histopathological results.Microvessels were immunohistochemical lablled with an antibody to CD₃₄. Microscope analysis was used to evaluate microvessel density (MVD). The study protocal was approved by the Ethical Review Board of Investigation in Human Being of Xiangan Hospital. Informed consent was obtained from each patients.

Results

The expression of leptin was significantly higher in CIN and cervical cancer groups than that in chronic cervicitis group(P<0.05). In addition, in cervical cancer group, there was no significant difference for leptin and CD₃₄ expression in different age and clinical stages(P>0.05), however, they were positively correlation with the degree of malignance and distant metastasis(P<0.05).

Conclusions

Leptin is an important vascular endothelial growth factor(VEGF) in cervical cancer and local overexpression of leptin in cervical cancer may contribute to tumor progression by increasing angiogenesis and tumor growth.

Key words: leptin, CD₃₄, microvessel density, cervical cancer, cervical intraepithelial neoplasia

表1 4组瘦素和CD₃₄表达情况比较[n(%)]

Table1 Comparison of expression of leptin and CD₃₄ among four groups[n(%)]

组别	n	瘦素表达					MVD值(/HP，±s)
组别	n	－	＋	＋＋	＋＋＋	阳性表达率	MVD值(/HP，±s)
宫颈炎组	15	11	4	0	0	4(26.7)	14.93±6.95
CINⅠ组	23	12	7	3	1	11(47.8)	16.12±7.35
CINⅡ组	25	10	9	3	3	15(60.0)	17.25±1.27
CINⅢ组	21	4	6	7	4	17(81.0)	19.38±6.74
宫颈癌组	58	2	10	21	25	56(96.6)	22.51±3.09

表1 4组瘦素和CD₃₄表达情况比较[n(%)]

Table1 Comparison of expression of leptin and CD₃₄ among four groups[n(%)]

组别	n	瘦素表达					MVD值(/HP，±s)
组别	n	－	＋	＋＋	＋＋＋	阳性表达率	MVD值(/HP，±s)
宫颈炎组	15	11	4	0	0	4(26.7)	14.93±6.95
CINⅠ组	23	12	7	3	1	11(47.8)	16.12±7.35
CINⅡ组	25	10	9	3	3	15(60.0)	17.25±1.27
CINⅢ组	21	4	6	7	4	17(81.0)	19.38±6.74
宫颈癌组	58	2	10	21	25	56(96.6)	22.51±3.09

图1 瘦素在宫颈炎组中的表达(×200，SP)

Figure 1 Leptin expression in cervicitis group(×200，SP)

图2 瘦素在CINⅠ组中的表达(×200，SP)

Figure 2 Leptin expression in CINⅠgroup(×200，SP)

图3 瘦素在CINⅡ组中的表达(×200，SP)

Figure 3 Leptin expression in CINⅡgroup(×200，SP)

图4 瘦素在CINⅢ组中的表达(×200，SP)

Figure 4 Leptin expression in CINⅢ group(×200，SP)

图5 瘦素在宫颈癌组中的表达(×200，SP)

Figure 5 Leptin expression in cervical cancer group(×200，SP)

图6 CD₃₄在宫颈癌组中的表达(×200，SP)

Figure 6 CD₃₄ expression in cervical cancer group(×200，SP)

表2 瘦素和CD₃₄表达的多因素logistic回归分析

Table 2 Multiple－factor logistic regression analysis of expression of leptin and CD₃₄

自变量	r	s	Wald值	P	OR	95%CI
自变量	r	s	Wald值	P	OR	Lower	Upper
年龄	0.263	0.633	0.570	0.676	1.153	0.374	4.527
分化程度	1.612	0.552	8.125	0.008	5.056	1.716	15.088
临床分期	0.606	0.471	1.103	0.058	1.735	0.749	4.621
淋巴结转移	3.849	0.714	18.742	0.001	14.352	5.307	28.292

表2 瘦素和CD₃₄表达的多因素logistic回归分析

Table 2 Multiple－factor logistic regression analysis of expression of leptin and CD₃₄

自变量	r	s	Wald值	P	OR	95%CI
自变量	r	s	Wald值	P	OR	Lower	Upper
年龄	0.263	0.633	0.570	0.676	1.153	0.374	4.527
分化程度	1.612	0.552	8.125	0.008	5.056	1.716	15.088
临床分期	0.606	0.471	1.103	0.058	1.735	0.749	4.621
淋巴结转移	3.849	0.714	18.742	0.001	14.352	5.307	28.292

[1]	Liu HL, Yang Y, Gao FY, et al.Expression and clinical significance of ABCG2 and CD₁₃₃ protein in cervical carcinoma[J]. Prog Obstet Gynecol, 2010, 19(2):115-118.
[2]	Weidner N. Intratumor microvessel density as a prognostic factor in cancer[J]. Am J Pathol, 1995, 147(1):9-19.
[3]	Cava AL, Alviggi C, Matarese G. Unraveling the multiple roles of leptin in inflammation and autoimmunity[J]. J Mol Med, 2004, 82:4-11.
[4]	Ruben RG, Amber W, Yanho X, et al. Leptin-signaling inhibition results in efficient anti-tumor activity in estrogen receptor positive or negative breast cancer[J]. Breast Cancer Res, 2009, 11(3):36-48.
[5]	Yin N, Wang D, Zhang H, et al. Molecular mechanisms involved in the growth stimulation of breast cancer cells by leptin[J]. Cancer Res, 2004, 64(16):5870-5875.
[6]	Hee SK. Leptin and leptin receptor expression in breast cancer[J]. Cancer Res Treat, 2009, 41(3):155-163.
[7]	Komal R, Heping Y, Meng X, et al. Leptin's mitogenic effect in human liver cancer cells requires induction of both methionine adenosyltransferase 2A and 2β[J].Hepatology, 2008, 47(2):521-531.
[8]	Giammarco F, Gianfranco A, Chiara R, et al. Leptin enhances cholangiocarcinoma cell growth[J]. Cancer Res, 2008, 68(16):6752-6761.
[9]	Vieira SC, Silva BB, Pinto GA, et al. CD₃₄ as a marker for evaluating angiogenesis in cervical cancer[J]. Pathol Res Pract, 2005, 201(4):313-318.
[10]	Suganami E, Takagi H, Ohashi H, et al.Leptin stimulates ischemia-induced retinal neovascularization: Possible role of vascular endothelial growth factor expressed in retinal endothelial cells[J]. Diabetes, 2004, 53(9):2443-2448.

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