Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2009, Vol. 05 ›› Issue (03): 246 -252. doi: 10.3877/cma.j.issn.1673-5250.2009.03.110

Original Article

Cyclooxygenase-2 Expression and Relation With Pathologic Parameters in Postmenopausal Endometrial Adenocarcinoma.

Yan WANG, Xuan WANG, Yong-li CHU   

  1. Department of Gynecology and Obstetrics, Qingdao University Medical College Affiliated Yantai Yu Huang Ding Hospital, Yantai 264000, Shandong Province, China
  • Published:2009-06-01
Objective

To investigate the expression and pathologic parameters of cyclooxygenase(COX)-2 in carcinogenesis of postmenopausal endometrial adenocarcinoma.

Methods

From April 2004 to February 2006, 30 patients with postmenopausal endometrial adenocarcinoma(EA group) and 10 patients with normal endometrial tissue(control group) were enrolled in this study in the Medical College of Qingdao University Affiliated Yantai Yu Huang Ding Hospital. Immunohistochemistry(IHC), reverse transcriptase-polymerase chain reaction(RT-PCR) and in situ hybridization histochemistry(ISHH) methods were used to detect the expression of cyclooxygenase-2 in 30 cases of postmenopausal endometrial adenocarcinoma tissues and 10 cases of normal endometrium tissues.

Results

① Immunohistochemistry: Cyclooxygenase-2 protein expression was significantly higher in endometrial adenocarcinoma than that of corresponding normal tissue(P<0.01); cyclooxygenase-2 protein expression significantly decreased in grade Ⅰ and Ⅱ than grade Ⅲ(P<0.01); cyclooxygenase-2 protein expression had no correlation to the pathological staging of endometrial adenocarcinoma(P>0.05). ② Reverse transcriptase-polymerase chain reaction: Cyclooxygenase-2 mRNA expression was stronger in endometrial adenocarcinoma than that of corresponding normal tissue(P<0.01); cyclooxygenase-2 mRNA expression was weaker in grade Ⅰ than grade Ⅱ and Ⅲ (P<0.01) and no correlation to the pathological staging of endometrial adenocarcinoma. ③ In-situ hybridization histochemistry: Cyclooxygenase-2 mRNA expression was stronger in endometrial adenocarcinoma than that of corresponding normal tissue(P<0.01); cyclooxygenase-2 mRNA expression was stronger with grade increased(P<0.01) and no correlation with pathological staging.

Conclusion

Cyclooxygenase-2 shows high expression of postmenopausal endometrial adenocarcinoma.Cyclooxygenase-2 may be involved in the course of tumor angiogenesis of postmenopausal endometrial adenocarcinoma. Cyclooxygenase-2 overexpresses in postmenopausal endometrial adenocarcinoma, and might participate in the process of tumor invasion and metastasis.

图1 正常子宫内膜组织环氧合酶–2蛋白表达(对照组,阴性,IHC×400)
图2 子宫内膜腺癌组织环氧合酶–2蛋白表达[病例组,阳性(胞浆),IHC×400(强)]
图3 子宫内膜腺癌组织COX–2 mRNA电泳图(RT–PCR) (病例组)
图4 正常子宫内膜组织COX–2 mRNA表达(对照组,阴性,ISHH×400)
图5 子宫内膜腺癌组织COX–2 mRNA的表达[病例组,阳性(胞浆),ISHH×400(强)]
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