Expression and Significance of Matrix Metalloproteinase-9, Cyclooxygenase-2 mRNA in Patients With Premature Rupture of Membranes

doi:10.3877/cma.j.issn.1673-5250.2013.04.005

Objective

To investigate the expression and its significance of human metalloproteinase-9, cyclooxygenase-2 in membrane of premature rupture of membranes (PROM) and normal term pregnancy.

Methods

We selected 35 subjects from the pregnant women of PROM in our hospital from September 2011 to February 2012 as research group. All subjects were divided into term PROM group (tPROM group, n=10) and preterm PROM group (pPROM group, n=25). The average age of tPROM group was (29.71±4.63) years old, and the average gestational age was (38.04±0.79) weeks. The average age of pPROM group was (30.67±4.05) years old, and the average gestational age was (33.56±0.82) weeks. Meanwhile, we selected 25 subjects from the healthy term-pregnant women as control group. The average age was (30.46±4.14) years old, the average gestational age was (38.91±0.99) weeks. This study followed the procedures established by the committee meet human trials hospital ethical standards, obtained its approval. All grouping subjects were informed and consent, and signed clinical research informed consent. The messenger RNA(mRNA) of MMP-9, COX-2 in fetal membranes by using real-time PCR in different groups were examined and compared.

Results

①The mRNA expressions of MMP-9 and COX-2 in fetal membranes were significantly high in pPROM group and tPROM group than those in control group (P<0.01), however, there were no significantly statistical difference between pPROM group and tPROM group (P>0.05). ②The relationship between MMP-9 and COX-2 in pPROM group and tPROM group was positively correlated (r=0.732, 0.820, P<0.05), there was no positive correlation in control group (r=0.244, P>0.05).

Conclusions

The mRNA expressions of MMP-9 and COX-2 in fetal membranes were significantly high in PROM, and they may play key roles in the occurrence of PROM.

Key words: premature rupture of membranes, matrix metalloproteinase-9, cyclooxygenase-2, real-time PCR

图1 GAPDH扩增曲线

Figure 1 The amplification curve of GAPDH

图2 GAPDH熔解曲线

Figure 2 The melting curve of GAPDH

图3 MMP-9扩增曲线

Figure 3 The amplification curve of MMP-9

图4 MMP-9熔解曲线

Figure 4 The melting curve of MMP-9

图5 COX-2扩增曲线

Figure 5 The amplification curve of COX-2

图6 COX-2熔解曲线

Figure 6 The melting curve of COX-2

表1 各组胎膜组织MMP-9 mRNA表达水平的比较 (±s)

Table 1 The expression level of MMP-9 mRNA in different fetal membrane tissues (±s)

组别	n	MMP-9 mRNA(ΔCT)	ΔΔCT	2^－ΔΔCT
tPROM组	25	3.0387±0.7987^a	－1.3094±0.7987	2.8117±1.4038
pPROM组	10	2.6769±0.5015^ab	－1.6713±0.5015	3.3596±1.1400
对照组	25	4.3482±0.3803	0±0.3803	1.0372±0.3179

表1 各组胎膜组织MMP-9 mRNA表达水平的比较 (±s)

Table 1 The expression level of MMP-9 mRNA in different fetal membrane tissues (±s)

组别	n	MMP-9 mRNA(ΔCT)	ΔΔCT	2^－ΔΔCT
tPROM组	25	3.0387±0.7987^a	－1.3094±0.7987	2.8117±1.4038
pPROM组	10	2.6769±0.5015^ab	－1.6713±0.5015	3.3596±1.1400
对照组	25	4.3482±0.3803	0±0.3803	1.0372±0.3179

表2 各组胎膜组织COX-2 mRNA的表达水平的比较(±s)

Table 2 The expression level of COX-2 mRNA in different fetal membrane tissues (±s)

组别	n	COX-2 mRNA(ΔCT)	ΔΔCT	2^－ΔΔCT
tPROM组	25	－10.0103±4.0547^a	－1.5291±0.4055	2.9873±0.7401^a
pPROM组	10	－10.1434±0.3799^ab	－1.6621±0.7799	3.2528±0.9559^{a b}
对照组	25	－8.4812±0.7248	0±0.7247	1.1037±0.4384

表2 各组胎膜组织COX-2 mRNA的表达水平的比较(±s)

Table 2 The expression level of COX-2 mRNA in different fetal membrane tissues (±s)

组别	n	COX-2 mRNA(ΔCT)	ΔΔCT	2^－ΔΔCT
tPROM组	25	－10.0103±4.0547^a	－1.5291±0.4055	2.9873±0.7401^a
pPROM组	10	－10.1434±0.3799^ab	－1.6621±0.7799	3.2528±0.9559^{a b}
对照组	25	－8.4812±0.7248	0±0.7247	1.1037±0.4384

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