Expression of Vascular Endothelial Growth Factor mRNA in Ovarian Neoplasm and Its Clinical Significance

doi:10.3877/cma.j.issn.1673-5250.2011.02.006

Objective

To investigate the expression of subtypes of vascular endothelial growth factor (VEGF) mRNA in ovarian neoplasm and its clinical significance.

Methods

From January 2000 to June 2004, tumor samples from 54 cases (research group) of ovarian malignant tumor were recruited into this study. Among them, ovarian epithelial carcinoma was 41 cases, and non-epithelial carcinoma was 13 (control group). The expressions of VEGF mRNA subtypes in ovarian malignant tumor and controls were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Meanwhile, an antibody to von willebrand factor (F8) was used to detect tumor microvessel density (MVD). The study protocol was approved by Beijing General Army Hospital. Informed consent was obtained from all participants.

Results

①The expression positive rates of VEGF 121, VEGF 165 in ovarian malignant were significantly higher than those in benign tumor and normal control (P<0.05); VEGF 189 were only expressed in ovarian malignant. ②No significantly association were between expressive levels of VEGF mRNA subtypes and clinical stage (χ²=0.259, P>0.05). ③The positive expression rate of VEGF 189 in patients with lymph node metastasis and omentum involvement were significantly higher than those in non-lymph node metastasis and normal omentum (P<0.05). ④There was a close relationship between VEGF 189 expression and microvessel density in ovarian malignant (P=0.003).

Conclusion

The expression of VEGF 121, 165 were correlated with the carcinogenesis and progress of ovarian malignant. VEGF 189 might be a biomarker for the status of ovarian carcinomas present in invasion and metastasis. VEGF 189 plays an important role in the angiogenesis of ovarian malignant.

Key words: vascular endothelial growth factor (VEGF), microvessel density (MVD), ovarian neoplasm, invasion, metastasis

图1　卵巢组织VEGF mRNA亚型表达聚丙烯酰胺凝胶银染电泳结果(lane1：对照组2(正常卵巢)仅表达VEGF 121; lane 2，4：恶性肿瘤，可见VEGF 121，165和189 3条带，VEGF 189呈弱表达；lane 3：对照组1(良性肿瘤), 可见VEGF 165，121表达2条带；lane 5 ：阴性对照；lane 6 ：100 bp DNAMarker; lane 7: 阳性对照)

图2　卵巢恶性肿瘤组织VEGF mRNA亚型表达聚丙烯酰胺凝胶银染电泳结果(lane 1，3: 无淋巴结转移卵巢恶性肿瘤组织；lane 2，6 ：阴性对照；lane 4，10：大网膜正常卵巢恶性肿瘤组织；lane 5：淋巴结转移卵巢恶性肿瘤组织；lane 7：大网膜转移卵巢恶性肿瘤组织可见VEGF 121，165和189表达3条带；lane 8 ：阳性对照；lane 9: 100 bp DNAMarker.)

图3　VEGF 189表达呈阳性的卵巢恶性肿瘤组织MVD: 镜下(×100)见微血管丰富，可见毛细血管簇及裂隙状血管(以F8为血管内皮细胞的标记物，SABC法染色测定卵巢恶性肿瘤组织MVD)

图4　VEGF表达呈阴性卵巢恶性肿瘤组织MVD：镜下(×100)见微血管稀疏(以F8为血管内皮细胞的标记物，SABC法染色卵巢恶性肿瘤组织MVD)

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