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中华妇幼临床医学杂志(电子版)

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2012, Vol. 08 ›› Issue (02): 179 -182. doi: 10.3877/cma.j.issn.1673-5250.2012.02.017

Special Issue: ；

Original Article

Vascular Endothelial Growth Factor and Its Receptors Expression and Microvessel Density in Ovarian Malignant Neoplasm

Li-mei WANG1, Xiao-yan LIU1, Shu-he WANG1, Li-xin SHANG1()   

  1. 1. Department of Gynecology and Obstetrics, Beijing General Army Hospital, Beijing 1007001, China
  • Received:2012-01-09 Revised:2012-03-03 Published:2012-04-01
  • Corresponding author: Li-xin SHANG
  • About author:
    Corresponding author: SHANG Li-xin, Email:
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Objective

To investigate the expression of vascular endothelial growth factor(VEGF) and its receptors (Flt-1, KDR/Flt-1) in ovarian carcinoma and its clinical significance and the relationship with tumor microvessel density (MVD).

Methods

This was a prospective observational study with institutional ethics approval and written all participants consent. From January 2000 to June 2004, a total of 48 cases of ovarian carcinoma who diagnosis in Beijing General Army Hospital recruited. Strept-avidin-birotin-peroxidase complex (SABC) immunohistochemistry method was used to determine the expression of VEGF and Flt-1, KDR/Flk-1 in 48 cases of ovarian carcinoma. Meanwhile an antibody to von willebrand factor (FⅧ) related antigen(FⅧ-RA)were also used to detect MVD.

Results

①No significantly association were between expressive levels of VEGF and Flt-1 , KDR with pathology types, differentiated grade and clinical stage of ovarian carcinoma(P>0.05); but VEGF and Flt-1 co-expression was associated with differentiated grade and clinical stage of ovarian carcinoma (P<0.05). ②VEGF and Flt-1 expression in lymph node metastasis was higher than that in no lymph node metastasis (P<0.05). KDR expression in metastasis liver tissue was higher than that in normal liver tissue(P=0.02), VEGF and KDR co-expression was associated with level metastasis (P=0.005). ③The positive expression rate of Flt-1 of patients with the ascite ≥1000 mL were significantly higher than that in patients with the ascite <1000 mL (P=0.01). ④There was also a close relationship between VEGF and KDR expression with MVD in ovarian carcinoma (P=0.03).

Conclusions

The expression of VEGF and its receptors according many ways associated with angiogenesis, metastasis and ascite in ovarian carcinoma, They might be a biomarker for the status of present in ovarian carcinoma metastasis and prognosis.

Key words: vascular endothelial growth factor, microvessel density, Flt-1, KDR/ Flt-1 receptors, ovarian carcinoma
表1 卵巢癌组织标本VEGF及其Flt－1，KDR/Flt－1受体蛋白表达与卵巢癌的临床病理关系[n(%)]
Table 1 Relationship of VEGF and Flt－1，KDR/Flt－1 expression with clinical significance in ovarian carcinoma [n(%)]
类别 n VEGF Flt－1 KDR/Flk－1 VEGF/Flt－1 VEGF/KDR
病理学类别 ? ? ? ? ? ?
? 上皮性 41 27(65.8) 24(58.8) 17(41.5) 21(51.2) 16(39.0)
? 非上皮性 7 5(71.4) 4(57.1) 4(57.1) 5(71.4) 3(42.8)
? P ? 0.880 0.730 0.730 0.580 0.800
组织学分化 ? ? ? ? ? ?
? G1，G2 14 8(57.1) 6(42.9) 5(35.7) 4(28.6) 3(21.4)
? G3/未 34 24(70.6) 22(64.7) 16(47.1) 22(64.7) 16(47.1)
? P ? 0.590 0.180 0.480 0.020 0.090
FIGO分期 ? ? ? ? ? ?
? Ⅰ，Ⅱ 16 9(56.2) 6(43.7) 7(43.8) 4(25.0) 4(25.0)
? Ⅲ，Ⅳ 32 23(71.9) 22(68.7) 14(43.8) 22(68.7) 15(46.9)
? P ? 0.270 0.770 0.950 0.005 0.160
表2 卵巢癌组织VEGF及其受体表达与远处转移及腹水的关系[n(%)]
Table 2 Relationship between the expressions of VEGF/ its receptor and the remote metastasis and volume of ascites in ovarian carcinoma [n(%)]
类别 n VEGF Flt－1 KDR/Flk－1 VEGF/Flt－1 VEGF/KDR
淋巴结转移 ? ? ? ? ? ?
? 有(＋) 28 24(85.7) 20(71.4) 15(53.4) 20(71.4) 13(46.4)
? 无(－) 20 8(40.0) 8(40.0) 6(30.0) 6(30.0) 6(30.0)
? P ? 0.009 0.020 0.100 0.005 0.250
肝转移 ? ? ? ? ? ?
? 有(＋) 8 6(75.0) 6(75.0) 7(87.5) 6(75.0) 7(87.5)
? 无(－) 40 26(65.0) 22(55.0) 14(35.0) 20(50.0) 12(30.0)
? P ? 0.900 0.500 0.020 0.390 0.005
腹水(mL) ? ? ? ? ? ?
? ≥1000 30 24(75.0) 21(70.0) 16(53.3) 21(70.0) 13(43.3)
? <1000 18 8(44.4) 7(38.9) 5(27.8) 5(27.8) 6(33.3)
? P ? 0.010 0.040 0.090 0.005 0.490
表3 VEGF及其受体表达与微血管密度的关系(±s)
Table 3 Relationship between the expression of VEGF and its receptors with microvessel density in ovarian carcinomas (±s)
类别 n 平均MVD(/mL) Kruskal－Wallis P
VEGF ? ? ?
? ＋ 32 19.51±8.69 (7.2～46.0) 0.01
? － 16 12.68±4.04(4.1～21.6) ?
KDR/Flk－1 ? ? ?
? ＋ 21 21.64±8.63(10.2～46.0) 0.03
? － 27 17.06±7.87 (4.1～37.8) ?
Flt－1 ? ? ?
? ＋ 28 19.19±9.53 (4.1～46.0) 0.54
? － 20 17.16±6.74(8.2～29.4) ?
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