Human leukocyte antigen-B and -DRB1 loci polymorphism in children with Henoch-Schönlein purpura in Liangshan area

doi:10.3877/cma.j.issn.1673-5250.2023.02.017

Objective

To investigate the human leukocyte antigen (HLA)-B and HLA-DRB1 loci polymorphisms in children with Henoch-Schönlein purpura (HSP) in Liangshan area.

Methods

A total of 159 cases of HSP children who were admitted to the Department of Pediatrics, The First People′s Hospital of Liangshan Yi Autonomous Prefecture from June 2021 to June 2022 were selected into this study and enrolled into study group. Meanwhile, 132 cases of healthy children who underwent physical examinations during the same period in the same hospital were enrolled as control group. Peripheral venous blood samples (3 mL) were collected from children in both groups for HLA gene typing, and the allele frequencies of HLA-B and HLA-DRB1 genes were statistically compared by chi-square test, continuity adjusted chi-square test, or Fisher′s exact probability method. The study protocol was approved by the Medical Ethics Committee of The First People′s Hospital of Liangshan Yi Autonomous Prefecture (Approval No.[2021]2), and informed consent was obtained from the parents of all participants. There were no statistically significant differences in composition ratio of gender and age between two groups (P>0.05).

Results

①There were no statistically significant differences in frequencies of 23 kinds of HLA-B allele genes, such as HLA-B*07, HLA-B*08, HLA-B*13, HLA-B*15, HLA-B*27, HLA-B*35, HLA-B*37, HLA-B*38, HLA-B*39, HLA-B*40, HLA-B*41, HLA-B*44, HLA-B*46, HLA-B*48, HLA-B*51, HLA-B*52, HLA-B*54, HLA-B*55, HLA-B*56, HLA-B*57, HLA-B*58, HLA-B*59, HLA-B*67 between two groups (P>0.05). ②The frequency of HLA-DRB1*01 allele gene in study group was 0.9% (3/318), which was significantly lower than that in control group (4.5%, 12/264), while the frequency of HLA-DRB1*11 allele gene in study group was 15.1% (48/318), which was significantly higher than that in control group (6.1%, 16/264), and both the differences between two groups were statistically significant (χ²=7.45, 12.03; P=0.006, 0.001). There were no statistically significant differences in frequencies of other HLA-DRB1 allele genes, such as HLA-DRB1*03, HLA-DRB1*04, HLA-DRB1*07, HLA-DRB1*08, HLA-DRB1*09, HLA-DRB1*10, HLA-DRB1*12, HLA-DRB1*13, HLA-DRB1*14, HLA-DRB1*15, HLA-DRB1*16 between two groups (P>0.05).

Conclusions

The distribution of 23 kinds of HLA-B allele genes including HLA-B*07, HLA-B*08 and so on of HSP children in Liangshan area is similar to that in healthy children. HLA-DRB1*01 allele gene may be a resistant gene in HSP children in Liangshan area, while HLA-DRB1*11 allele gene may be its susceptible gene.

Key words: Purpura, Schoenlein-Henoch, HLA-B antigens, HLA-DR antigens, HLA-B gene, HLA-DRB1 gene, Gene polymorphism, Liangshan area, Child

表1 2组受试儿一般临床资料比较

组别	例数	性别[例数(%)]		年龄(岁，±s)
组别	例数	男	女	年龄(岁，±s)
研究组	159	84(52.8)	75(47.2)	8.3±1.9
对照组	132	70(53.0)	62(47.0)	8.6±2.3
统计量		χ²<0.01		t＝1.22
P值		0.973		0.224

表1 2组受试儿一般临床资料比较

组别	例数	性别[例数(%)]		年龄(岁，±s)
组别	例数	男	女	年龄(岁，±s)
研究组	159	84(52.8)	75(47.2)	8.3±1.9
对照组	132	70(53.0)	62(47.0)	8.6±2.3
统计量		χ²<0.01		t＝1.22
P值		0.973		0.224

表2 2组受试儿HLA-B等位基因频率比较[例数(%)]

组别	例数	HLA-B*07	HLA-B*08	HLA-B*13	HLA-B*15	HLA-B*27	HLA-B*35	HLA-B*37	HLA-B*38
研究组	159	12(3.8)	1(0.3)	18(5.7)	57(17.9)	11(3.5)	16(5.0)	4(1.3)	10(3.1)
对照组	132	14(5.3)	1(0.4)	13(4.9)	43(16.3)	9(3.4)	7(2.7)	4(1.5)	12(4.5)
χ²值		0.79	—^a	0.16	0.27	<0.01	2.15	0.01^b	0.78
P值		0.374	0.500	0.694	0.602	0.974	0.142	0.920	0.378
组别	例数	HLA-B*39	HLA-B*40	HLA-B*41	HLA-B*44	HLA-B*46	HLA-B*48	HLA-B*51	HLA-B*52
研究组	159	13(4.1)	52(16.4)	0(0)	3(0.9)	23(7.2)	6(1.9)	33(10.4)	16(5.0)
对照组	132	6(2.3)	39(14.8)	1(0.4)	3(1.1)	25(9.5)	5(1.9)	30(11.4)	10(3.8)
χ²值		1.51	0.27	—^a	0.03^b	0.95	0.09^b	0.15	0.52
P值		0.220	0.601	0.454	0.862	0.329	0.764	0.703	0.470
组别	例数	HLA-B*54	HLA-B*55	HLA-B*56	HLA-B*57	HLA-B*58	HLA-B*59	HLA-B*67
研究组	159	8(2.5)	17(5.3)	3(0.9)	0(0)	14(4.4)	0(0)	1(0.3)
对照组	132	7(2.7)	10(3.8)	1(0.4)	2(0.8)	19(7.2)	1(0.4)	2(0.8)
χ²值		0.01	0.79	0.10^b	—^a	2.11	—^a	0.03
P值		0.918	0.374	0.751	0.205	0.147	0.454	0.871

表2 2组受试儿HLA-B等位基因频率比较[例数(%)]

组别	例数	HLA-B*07	HLA-B*08	HLA-B*13	HLA-B*15	HLA-B*27	HLA-B*35	HLA-B*37	HLA-B*38
研究组	159	12(3.8)	1(0.3)	18(5.7)	57(17.9)	11(3.5)	16(5.0)	4(1.3)	10(3.1)
对照组	132	14(5.3)	1(0.4)	13(4.9)	43(16.3)	9(3.4)	7(2.7)	4(1.5)	12(4.5)
χ²值		0.79	—^a	0.16	0.27	<0.01	2.15	0.01^b	0.78
P值		0.374	0.500	0.694	0.602	0.974	0.142	0.920	0.378
组别	例数	HLA-B*39	HLA-B*40	HLA-B*41	HLA-B*44	HLA-B*46	HLA-B*48	HLA-B*51	HLA-B*52
研究组	159	13(4.1)	52(16.4)	0(0)	3(0.9)	23(7.2)	6(1.9)	33(10.4)	16(5.0)
对照组	132	6(2.3)	39(14.8)	1(0.4)	3(1.1)	25(9.5)	5(1.9)	30(11.4)	10(3.8)
χ²值		1.51	0.27	—^a	0.03^b	0.95	0.09^b	0.15	0.52
P值		0.220	0.601	0.454	0.862	0.329	0.764	0.703	0.470
组别	例数	HLA-B*54	HLA-B*55	HLA-B*56	HLA-B*57	HLA-B*58	HLA-B*59	HLA-B*67
研究组	159	8(2.5)	17(5.3)	3(0.9)	0(0)	14(4.4)	0(0)	1(0.3)
对照组	132	7(2.7)	10(3.8)	1(0.4)	2(0.8)	19(7.2)	1(0.4)	2(0.8)
χ²值		0.01	0.79	0.10^b	—^a	2.11	—^a	0.03
P值		0.918	0.374	0.751	0.205	0.147	0.454	0.871

表3 2组受试儿HLA-DRB1等位基因频率比较[例数(%)]

组别	例数	HLA-DRB1*01	HLA-DRB1*03	HLA-DRB1*04	HLA-DRB1*07	HLA-DRB1*08	HLA-DRB1*09	HLA-DRB1*10
研究组	159	3(0.9)	9(2.8)	59(18.6)	4(1.3)	26(8.2)	32(10.1)	4(1.3)
对照组	132	12(4.5)	11(4.2)	36(13.6)	8(3.0)	29(11.0)	31(11.7)	7(2.7)
χ²值		7.45	0.78	2.55	2.24	1.33	0.43	0.85^a
P值		0.006	0.378	0.110	0.134	0.249	0.516	0.356
组别	例数	HLA-DRB1*11	HLA-DRB1*12	HLA-DRB1*13	HLA-DRB1*14	HLA-DRB1*15	HLA-DRB1*16
研究组	159	48(15.1)	59(18.6)	8(2.5)	34(10.7)	26(8.2)	6(1.9)
对照组	132	16(6.1)	42(15.9)	8(3.0)	24(9.1)	30(11.4)	10(3.8)
χ²值		12.03	0.70	0.14	0.41	1.69	1.95
P值		0.001	0.402	0.705	0.521	0.194	0.163

表3 2组受试儿HLA-DRB1等位基因频率比较[例数(%)]

组别	例数	HLA-DRB1*01	HLA-DRB1*03	HLA-DRB1*04	HLA-DRB1*07	HLA-DRB1*08	HLA-DRB1*09	HLA-DRB1*10
研究组	159	3(0.9)	9(2.8)	59(18.6)	4(1.3)	26(8.2)	32(10.1)	4(1.3)
对照组	132	12(4.5)	11(4.2)	36(13.6)	8(3.0)	29(11.0)	31(11.7)	7(2.7)
χ²值		7.45	0.78	2.55	2.24	1.33	0.43	0.85^a
P值		0.006	0.378	0.110	0.134	0.249	0.516	0.356
组别	例数	HLA-DRB1*11	HLA-DRB1*12	HLA-DRB1*13	HLA-DRB1*14	HLA-DRB1*15	HLA-DRB1*16
研究组	159	48(15.1)	59(18.6)	8(2.5)	34(10.7)	26(8.2)	6(1.9)
对照组	132	16(6.1)	42(15.9)	8(3.0)	24(9.1)	30(11.4)	10(3.8)
χ²值		12.03	0.70	0.14	0.41	1.69	1.95
P值		0.001	0.402	0.705	0.521	0.194	0.163

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