Human leukocyte antigen-B and -DRB1 loci polymorphism in children with Henoch-Schönlein purpura in Liangshan area

doi:10.3877/cma.j.issn.1673-5250.2023.02.017

Objective

To investigate the human leukocyte antigen (HLA)-B and HLA-DRB1 loci polymorphisms in children with Henoch-Schönlein purpura (HSP) in Liangshan area.

Methods

A total of 159 cases of HSP children who were admitted to the Department of Pediatrics, The First People′s Hospital of Liangshan Yi Autonomous Prefecture from June 2021 to June 2022 were selected into this study and enrolled into study group. Meanwhile, 132 cases of healthy children who underwent physical examinations during the same period in the same hospital were enrolled as control group. Peripheral venous blood samples (3 mL) were collected from children in both groups for HLA gene typing, and the allele frequencies of HLA-B and HLA-DRB1 genes were statistically compared by chi-square test, continuity adjusted chi-square test, or Fisher′s exact probability method. The study protocol was approved by the Medical Ethics Committee of The First People′s Hospital of Liangshan Yi Autonomous Prefecture (Approval No.[2021]2), and informed consent was obtained from the parents of all participants. There were no statistically significant differences in composition ratio of gender and age between two groups (P>0.05).

Results

①There were no statistically significant differences in frequencies of 23 kinds of HLA-B allele genes, such as HLA-B*07, HLA-B*08, HLA-B*13, HLA-B*15, HLA-B*27, HLA-B*35, HLA-B*37, HLA-B*38, HLA-B*39, HLA-B*40, HLA-B*41, HLA-B*44, HLA-B*46, HLA-B*48, HLA-B*51, HLA-B*52, HLA-B*54, HLA-B*55, HLA-B*56, HLA-B*57, HLA-B*58, HLA-B*59, HLA-B*67 between two groups (P>0.05). ②The frequency of HLA-DRB1*01 allele gene in study group was 0.9% (3/318), which was significantly lower than that in control group (4.5%, 12/264), while the frequency of HLA-DRB1*11 allele gene in study group was 15.1% (48/318), which was significantly higher than that in control group (6.1%, 16/264), and both the differences between two groups were statistically significant (χ²=7.45, 12.03; P=0.006, 0.001). There were no statistically significant differences in frequencies of other HLA-DRB1 allele genes, such as HLA-DRB1*03, HLA-DRB1*04, HLA-DRB1*07, HLA-DRB1*08, HLA-DRB1*09, HLA-DRB1*10, HLA-DRB1*12, HLA-DRB1*13, HLA-DRB1*14, HLA-DRB1*15, HLA-DRB1*16 between two groups (P>0.05).

Conclusions

The distribution of 23 kinds of HLA-B allele genes including HLA-B*07, HLA-B*08 and so on of HSP children in Liangshan area is similar to that in healthy children. HLA-DRB1*01 allele gene may be a resistant gene in HSP children in Liangshan area, while HLA-DRB1*11 allele gene may be its susceptible gene.

Key words: Purpura, Schoenlein-Henoch, HLA-B antigens, HLA-DR antigens, HLA-B gene, HLA-DRB1 gene, Gene polymorphism, Liangshan area, Child

表1 2组受试儿一般临床资料比较

组别	例数	性别[例数(%)]		年龄(岁，±s)
组别	例数	男	女	年龄(岁，±s)
研究组	159	84(52.8)	75(47.2)	8.3±1.9
对照组	132	70(53.0)	62(47.0)	8.6±2.3
统计量		χ²<0.01		t＝1.22
P值		0.973		0.224

表1 2组受试儿一般临床资料比较

组别	例数	性别[例数(%)]		年龄(岁，±s)
组别	例数	男	女	年龄(岁，±s)
研究组	159	84(52.8)	75(47.2)	8.3±1.9
对照组	132	70(53.0)	62(47.0)	8.6±2.3
统计量		χ²<0.01		t＝1.22
P值		0.973		0.224

表2 2组受试儿HLA-B等位基因频率比较[例数(%)]

组别	例数	HLA-B*07	HLA-B*08	HLA-B*13	HLA-B*15	HLA-B*27	HLA-B*35	HLA-B*37	HLA-B*38
研究组	159	12(3.8)	1(0.3)	18(5.7)	57(17.9)	11(3.5)	16(5.0)	4(1.3)	10(3.1)
对照组	132	14(5.3)	1(0.4)	13(4.9)	43(16.3)	9(3.4)	7(2.7)	4(1.5)	12(4.5)
χ²值		0.79	—^a	0.16	0.27	<0.01	2.15	0.01^b	0.78
P值		0.374	0.500	0.694	0.602	0.974	0.142	0.920	0.378
组别	例数	HLA-B*39	HLA-B*40	HLA-B*41	HLA-B*44	HLA-B*46	HLA-B*48	HLA-B*51	HLA-B*52
研究组	159	13(4.1)	52(16.4)	0(0)	3(0.9)	23(7.2)	6(1.9)	33(10.4)	16(5.0)
对照组	132	6(2.3)	39(14.8)	1(0.4)	3(1.1)	25(9.5)	5(1.9)	30(11.4)	10(3.8)
χ²值		1.51	0.27	—^a	0.03^b	0.95	0.09^b	0.15	0.52
P值		0.220	0.601	0.454	0.862	0.329	0.764	0.703	0.470
组别	例数	HLA-B*54	HLA-B*55	HLA-B*56	HLA-B*57	HLA-B*58	HLA-B*59	HLA-B*67
研究组	159	8(2.5)	17(5.3)	3(0.9)	0(0)	14(4.4)	0(0)	1(0.3)
对照组	132	7(2.7)	10(3.8)	1(0.4)	2(0.8)	19(7.2)	1(0.4)	2(0.8)
χ²值		0.01	0.79	0.10^b	—^a	2.11	—^a	0.03
P值		0.918	0.374	0.751	0.205	0.147	0.454	0.871

表2 2组受试儿HLA-B等位基因频率比较[例数(%)]

组别	例数	HLA-B*07	HLA-B*08	HLA-B*13	HLA-B*15	HLA-B*27	HLA-B*35	HLA-B*37	HLA-B*38
研究组	159	12(3.8)	1(0.3)	18(5.7)	57(17.9)	11(3.5)	16(5.0)	4(1.3)	10(3.1)
对照组	132	14(5.3)	1(0.4)	13(4.9)	43(16.3)	9(3.4)	7(2.7)	4(1.5)	12(4.5)
χ²值		0.79	—^a	0.16	0.27	<0.01	2.15	0.01^b	0.78
P值		0.374	0.500	0.694	0.602	0.974	0.142	0.920	0.378
组别	例数	HLA-B*39	HLA-B*40	HLA-B*41	HLA-B*44	HLA-B*46	HLA-B*48	HLA-B*51	HLA-B*52
研究组	159	13(4.1)	52(16.4)	0(0)	3(0.9)	23(7.2)	6(1.9)	33(10.4)	16(5.0)
对照组	132	6(2.3)	39(14.8)	1(0.4)	3(1.1)	25(9.5)	5(1.9)	30(11.4)	10(3.8)
χ²值		1.51	0.27	—^a	0.03^b	0.95	0.09^b	0.15	0.52
P值		0.220	0.601	0.454	0.862	0.329	0.764	0.703	0.470
组别	例数	HLA-B*54	HLA-B*55	HLA-B*56	HLA-B*57	HLA-B*58	HLA-B*59	HLA-B*67
研究组	159	8(2.5)	17(5.3)	3(0.9)	0(0)	14(4.4)	0(0)	1(0.3)
对照组	132	7(2.7)	10(3.8)	1(0.4)	2(0.8)	19(7.2)	1(0.4)	2(0.8)
χ²值		0.01	0.79	0.10^b	—^a	2.11	—^a	0.03
P值		0.918	0.374	0.751	0.205	0.147	0.454	0.871

表3 2组受试儿HLA-DRB1等位基因频率比较[例数(%)]

组别	例数	HLA-DRB1*01	HLA-DRB1*03	HLA-DRB1*04	HLA-DRB1*07	HLA-DRB1*08	HLA-DRB1*09	HLA-DRB1*10
研究组	159	3(0.9)	9(2.8)	59(18.6)	4(1.3)	26(8.2)	32(10.1)	4(1.3)
对照组	132	12(4.5)	11(4.2)	36(13.6)	8(3.0)	29(11.0)	31(11.7)	7(2.7)
χ²值		7.45	0.78	2.55	2.24	1.33	0.43	0.85^a
P值		0.006	0.378	0.110	0.134	0.249	0.516	0.356
组别	例数	HLA-DRB1*11	HLA-DRB1*12	HLA-DRB1*13	HLA-DRB1*14	HLA-DRB1*15	HLA-DRB1*16
研究组	159	48(15.1)	59(18.6)	8(2.5)	34(10.7)	26(8.2)	6(1.9)
对照组	132	16(6.1)	42(15.9)	8(3.0)	24(9.1)	30(11.4)	10(3.8)
χ²值		12.03	0.70	0.14	0.41	1.69	1.95
P值		0.001	0.402	0.705	0.521	0.194	0.163

表3 2组受试儿HLA-DRB1等位基因频率比较[例数(%)]

组别	例数	HLA-DRB1*01	HLA-DRB1*03	HLA-DRB1*04	HLA-DRB1*07	HLA-DRB1*08	HLA-DRB1*09	HLA-DRB1*10
研究组	159	3(0.9)	9(2.8)	59(18.6)	4(1.3)	26(8.2)	32(10.1)	4(1.3)
对照组	132	12(4.5)	11(4.2)	36(13.6)	8(3.0)	29(11.0)	31(11.7)	7(2.7)
χ²值		7.45	0.78	2.55	2.24	1.33	0.43	0.85^a
P值		0.006	0.378	0.110	0.134	0.249	0.516	0.356
组别	例数	HLA-DRB1*11	HLA-DRB1*12	HLA-DRB1*13	HLA-DRB1*14	HLA-DRB1*15	HLA-DRB1*16
研究组	159	48(15.1)	59(18.6)	8(2.5)	34(10.7)	26(8.2)	6(1.9)
对照组	132	16(6.1)	42(15.9)	8(3.0)	24(9.1)	30(11.4)	10(3.8)
χ²值		12.03	0.70	0.14	0.41	1.69	1.95
P值		0.001	0.402	0.705	0.521	0.194	0.163

[1]	Barnhart ML, Rosenbaum K. Anaphylactoid syndrome of pregnancy[J]. Nurs Womens Health, 2019, 23(1): 38-48. DOI: 10.1016/j.nwh.2018.11.006.
[2]	王庆谊，孟昭影. 过敏性紫癜发病机制的研究进展[J]. 中国中西医结合皮肤性病学杂志，2020, 19(3): 285-287. DOI: 10.3969/j.issn.1672-0709.2020.03.028.
[3]	杜娟，姚新生，于红松. HLA基因与常见自身免疫病相关性研究进展[J]. 中国免疫学杂志，2019, 35(1): 118-122, 128. DOI: 10.3969/j.issn.1000-484X.2019.01.025.
[4]	López-Mejías R, Castañeda S, Genre F, et al. Genetics of immunoglobulin-A vasculitis (Henoch-Schönlein purpura): an updated review[J]. Autoimmun Rev, 2018, 17(3): 301-315. DOI: 10.1016/j.autrev.2017.11.024.
[5]	王天有，申昆玲，沈颖，等. 诸福棠实用儿科学[M]. 9版. 北京：人民卫生出版社，2022: 875.
[6]	He X, Yu C, Zhao P, et al. The genetics of Henoch-Schönlein purpura: a systematic review and Meta-analysis[J]. Rheumatol Int, 2013, 33(6): 1387-1395. DOI: 10.1007/s00296-012-2661-4.
[7]	Hetland LE, Susrud KS, Lindahl KH，et al. Henoch-Schönlein purpura: a literature review[J]. Acta Derm Venereol, 2017, 97(10): 1160-1166. DOI: 10.2340/00015555-2733.
[8]	Oni L, Sampath S. Childhood IgA vasculitis (Henoch Schonlein purpura)-advances and knowledge gaps[J]. Front Pediatr, 2019, 7: 257. DOI: 10.3389/fped.2019.00257.
[9]	Reid-Adam J. Henoch-Schonlein purpura[J]. Pediatr Rev, 2014, 35(10): 447-449, discussion 449. DOI: 10.1542/pir.35-10-447.
[10]	孙小妹，戴亮，孙飞扬，等. 成都地区与川西高原地区过敏性紫癜患儿临床特征比较分析[J]. 现代预防医学，2018, 45(12): 2274-2276, 2287.
[11]	李克莉，刘大卫，武文娣，等. 中国六个市2007~2009年过敏性紫癜住院病例发病情况分析[J]. 中国疫苗和免疫，2011, 17(2): 128-132.
[12]	Wei CC, Lin CL, Shen TC, et al. Atopic dermatitis and association of risk for Henoch-Schönlein purpura (IgA vasculitis) and renal involvement among children: results from a population-based cohort study in Taiwan[J]. Medicine (Baltimore), 2016, 95(3): e2586. DOI: 10.1097/MD.0000000000002586.
[13]	钟芳芳，杨友，郭铃，等. IL-17A基因多态性与过敏性紫癜易感性及紫癜性肾炎的相关性研究[J]. 川北医学院学报，2022, 37(1): 1-5. DOI: 10.3969/j.issn.1005-3697.2022.01.001.
[14]	全湛柔，邓志辉，周丹，等. 中国南方汉族造血干细胞捐献者HLA-A、-B、-C、-DRB1及-DQB1高分辨等位基因多态性分析[J]. 国际输血及血液学杂志，2018, 41(6): 497-505. DOI: 10.3760/cma.j.issn.1673-419X.2018.06.007.
[15]	朱传福，聂向民，宋永红，等. 中国造血干细胞捐献人群37个HLA新等位基因形成的分子机制分析[J]. 国际输血及血液学杂志，2022, 45(1): 68-73. DOI: 10.3760/cma.j.cn511693-20200916-00206.
[16]	金士正，邹红岩，甄建新，等. 中国南方汉族人群HLA-Ⅰ、Ⅱ类八个位点等位基因多态性的研究[J]. 中华医学遗传学杂志，2017, 34(1): 110-114. DOI: 10.3760/cma.j.issn.1003-9406.2017.01.026.
[17]	Nyulassy S, Buc M, Sasinka M, et al. The HLA system in glomerulonephritis[J]. Clin Immunol Immunopathol, 1977, 7(3): 319-323. DOI: 10.1016/0090-1229(77)90064-2.
[18]	Rashidi S, Shiari R, Farivar S. HLA-DRB1 gene polymorphisms in Iranian children with Henoch-Schönlein purpura[J]. J Res Med Sci, 2018, 23: 42. DOI: 10.4103/jrms.JRMS_344_17.
[19]	闫亚飞. 宁夏地区回汉儿童HLA基因多态性研究及其与体质和紫癜的相关性[D]. 银川：宁夏医科大学，2017.
[20]	López-Mejías R, Genre F, Pérez BS, et al. Association of HLA-B41：02 with Henoch-Schönlein purpura (IgA vasculitis) in Spanish individuals irrespective of the HLA-DRB1* status[J]. Arthritis Res Ther, 2015, 17(1):102-108. DOI: 10.1002/art.38979.
[21]	任少敏，杨光路，刘春枝，等. 内蒙地区蒙汉族儿童过敏性紫癜HLA-A、B关联基因的探讨及测序分析[J]. 现代免疫学，2009, 29(1): 68-70.
[22]	贺少军，陶仲宾，凌继祖，等. HLA基因与过敏性紫癜及其肾脏损害遗传易感性的研究进展[J]. 医学综述，2022, 28(10): 1901-1905. DOI: 10.3969/j.issn.1006-2084.2022.10.006.

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