Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2016, Vol. 12 ›› Issue (04): 383 -389. doi: 10.3877/cma.j.issn.1673-5250.2016.04.003

Special Issue:

Original Article

Effect of polluted pollen by vehicle exhaust on T helper cell 17/regulatory T cell imbalance in asthmatic mice

Ling Yang1, Xinxin Xu1, Yan Zhou1, Jianqiang Kang1,(), Ye Tian1   

  1. 1. Department of Geriatrics, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Received:2016-03-18 Revised:2016-06-20 Published:2016-08-01
  • Corresponding author: Jianqiang Kang
  • About author:
    Corresponding author:Kang Jianqiang, Email:
Objective

To investigate the effect of polluted pollen by vehicle exhaust on T helper cell(Th)17/regulatory T cell(Treg) imbalance in asthmatic mice.

Methods

A total of 60 cases of 6-8 weeks of age male BALB/c mice were chosen as study objects.According to random digits table, they were randomly divided into A to D groups and control group, 12 mice in each group. In group A, mice were treated with Artemisia pollen extracts as allergen and stimulator. In group B, mice were treated with Artemisia pollen extracts and polluted Artemisia pollen extracts by vehicle exhaust as allergen and stimulator, respectively. In group C, mice were treated with polluted Artemisia pollen extracts by vehicle exhaust and Artemisia pollen extracts as allergen and stimulator, respectively.In group D, mice were treated with polluted Artemisia pollen extracts by vehicle exhaust as allergen and stimulator. In control group, mice were treated with phosphate buffered saline(PBS) as allergen and stimulator. Lung tissues, bronchoalveolar lavage fluid(BALF) and spleen tissues were collected from the 5 groups and processed as following: ①Histopathological changes in lung tissue of mice in different groups were observed at high magnification by hematoxylin-eosin(HE) staining.②Statistical differences among 5 groups in levels of IL-17A, -10 and the cell countings and differentiations in BALF were compared.③Statistical differences of ratios of Th17 to CD4+ T cells, Treg cells to CD4+ T cells and Th17/Treg in the spleens mononuclearcell among 5 groups were also compared. The disposition of the animals in experimental process was in accordance with the ethical standard of animals.

Results

①In asthmatic mice of groups A to D, varying degrees of bronchiolar contraction and inflammatory cellular infiltration were observed, and all were the most serious in group D. ②In BALF of asthmatic mice of group A to D, higher IL-17A and lower IL-10 contents than those of control group, and the differences were statistically significant(P<0.01); In BALF of asthmatic mice of group A to C, lower IL-17A and higher IL-10 contents than those of group D, and the differences were statistically significant(P<0.05); The total cells, eosinophils(EOS) and neutrophils(NEU) counts in BALF of asthmatic mice of group A to D were higher than those of control group, EOS and NEU counts in BALF of asthmatic mice of group A to C were lower than those of group D, and the differences were statistically significant(P<0.05). ③The ratio of Th17 cells to CD4+ T cells and Th17/Treg in the spleens mononuclearcell of asthmatic mice of group A to D were higher than those of control group, and ratio of Treg to CD4+ T cells was lower than that of control group, and the differences were statistically significant(P<0.01); The ratio of Th17/Treg of group D was higher than that of group A, and the difference was statistically significant(P<0.05).

Conclusions

The cellular immunity imbalance is more severe in asthma mice which is induced by polluted pollen by vehicle exhaust, and cause more inflammatory mediators released, resulting in more severe airway inflammation.

图1 5组小鼠肺组织病理学改变(图1A~1E:分别为对照组及A、B、C与D组)(HE染色,高倍镜)
表1 5组小鼠BALF中IL-17A、-10含量及细胞计数、分类比较(±s)
表2 各组小鼠脾脏单个核细胞中Th17及Treg细胞占CD4+T细胞比例和Th17/Treg比较(±s)
图2 小鼠脾脏单个核细胞Th17、Treg流式细胞图(图2A~2C:分别为对照组、A组及D组的Th17细胞;图2D~2F:分别为对照组、A组及D组的Treg细胞)
[1]
胡斯明,罗雅玲,赖文岩,等.调节性T细胞/Th17在支气管哮喘小鼠气道炎症过程中的变化[J].中国现代医学杂志,2009,19(19):2881-2884.
[2]
龚臣,邓静敏. Th17/Treg在支气管哮喘发病机制中的作用及研究进展[J/CD]. 中华哮喘杂志:电子版,2013,7(3):41-45.
[3]
Svartengren M, Strand V, Bylin G, et al. Short-term exposure to air pollution in a road tunnel enhances the asthmatic response to allergen[J]. Eur Respir J, 2000, 15(4):716-724.
[4]
Gruijthuijsen YK, Grieshuber I, Stöcklinger A, et al. Nitration enhances the allergenic potential of proteins[J]. Int Arch Allergy Immunol, 2006, 141(3): 265-275.
[5]
杨玲,周琪,李强.汽车尾气对常见过敏原硝化作用[J].中国公共卫生,2012,28(增刊):41-42.
[6]
姚丽娜,张宏誉.气传花粉监测[J].中华临床免疫和变态反应杂志,2009,3(4):295-300.
[7]
温志华,尹佳. 蒿属、葎草花粉空气浓度与夏秋季花粉症患者哮喘症状严重程度的相关性[J]. 中华临床免疫和变态反应杂志,2012,6(1):10-17.
[8]
Asero R, Wopfner N, Gruber P, et al. Artemisia and Ambrosia hypersensitivity:co-sensitization or co-recognition?[J]. Clin Exp Allergy, 2006, 36(5):658-665.
[9]
D′Amato G, Cecchi L, D′Amato M, et al.Urban air pollution and climate change as environmental risk factors of respiratory allergy:an update[J]. J Investig Allergol Clin Immunol, 2010, 20(2):95-102.
[10]
Franze T, Weller MG, Niessner R, et al. Protein nitration by polluted air[J]. Environ Sci Technol, 2005, 39(6):1673-1678.
[11]
Brunekreef B, Sunyer J. Asthma, rhinitis and air pollution:is traffic to blame?[J]. Eur Respir J, 2003, 21(6):913-915.
[12]
Fernvik E, Peltre G, Sénéchal H, et al. Effects of birch pollen and traffic particulate matter on Th2 cytokines, immunoglobulin E levels and bronchial hyper-responsiveness in mice[J]. Clin Exp Allergy, 2002, 32(4):602-611.
[13]
Bettelli E, Carrier Y, Gao W, et al. Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells[J]. Nature, 2006, 441(7090): 235-238.
[14]
周辰,殷凯生,陈军浩,等. 辅助性T细胞17及IL-17在支气管哮喘小鼠中的变化[J]. 南京医科大学学报:自然科学版,2009,29(8):1095-1098,1123.
[15]
Cheung PF, Wong CK, Lam CW.Molecular mechanisms of cytokine and chemokine release from eosinophils activated by IL-17A, IL-17F, and IL-23:implication for Th17 lymphocytes-mediated allergic inflammation[J]. J Immunol, 2008, 180(8):5625-5635.
[16]
Bullens DM, Truyen E, Coteur L, et al. IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulocytic influx? [J] Respir Res, 2006, 7:135.
[17]
Nguyen KD, Fohner A, Booker JD, et al. XCL1 enhances regulatory activities of CD4+ CD25high CD127low/- T cells in human allergic asthma[J]. J Immunol, 2008, 181(8):5386-5395.
[18]
Shi YH, Shi GC, Wan HY, et al.Coexistence of Th1/Th2 and Th17/Treg imbalances in patients with allergic asthma[J]. Chin Med J(Engl), 2011, 124(13):1951-1956.
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