Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2009, Vol. 05 ›› Issue (02): 114 -118. doi: 10.3877/cma.j.issn.1673-5250.2009.02.104

Original Article

Effects of Exogenous Hydrogen Sulfide on the Expression of Lung Muc5ac in Ovalbumin-Induced Asthma Rats.

Qiao-bin CHEN, Lang CHEN, Yu LIN, Hui CHEN   

  1. Department of Pediatrics, Fujian Provincal Hospital, Fuzhou 350001, China
  • Published:2009-04-01
  • Supported by:
    * Project No.2006–1–30, supported by the Science Fund for Young Scholars of Fujian Province
Objective

To explore effects of gaseous signal molecule hydrogen sulfide (H2S) on the lung Muc5ac expression of ovalbumin (OVA)-induced asthma rats.

Methods

Twenty-six healthy SD rats were randomly divided into 3 groups, asthma group (ovalbumin-induced, n=9), NaHS group(ovalbumin-induced rats treated with NaHS, n=9), and the control group(n=8). After treating for 24 hours, rats were anatomized to observe and the degree of infiltration of inflammatory cells around bronchus were scored with optical microscope. The expression of lung Muc5ac were determined by the method of immunohistochemical staining.

Results

The score of the degree of infiltration of inflammatory cells around bronchus expressed by median were 4 in asthma group, 2 in NaHS group, and 1 in the control group. Compared with 3 groups, there were statistical significance (H=13.75, P<0.05). The protein content of lung muc5ac expressed with positive areas were (1 330.22±662.87) μm2, (715.88±282.49) μm2 and (501.75±270.58) μm2 in asthma group, NaHS group and the control group, respectively. There were significant difference compared within 3 groups(F=13.75, P<0.01).

Conclusion

The expression of lung Muc5ac increases in ovalbumin-induced asthma rats. Exogenous hydrogen sulfide may play an important role in alleviate airway inflammation and protect from asthma acute break out.

1 Gao YD, Xiong SD, Xu YJ. Effects of endogenous nitric oxide on potassium channels of bronchial smooth muscle cells from asthmatic rats. Chin J Pathophysiol, 2005, 21(10): 1974–1978.[高亚东,熊盛道,徐永健. 内源性一氧化氮对哮喘大鼠支气管平滑肌细胞钾通道的影响. 中国病理生理杂志,2005,21(10): 1974–1978.]
2 Zhang ML, Tang BX. Level and significance of endogeous carbon monoxide of patients with asthma. Chin J Misdiag, 2004, 4(2): 179–181.[张曼林,汤兵祥. 哮喘患者血浆内源性一氧化碳的水平及意义. 中国误诊学杂志,2004, 4(2): 179–181.]
3 Zeidler MR, Kleerup EC, Tashkin DP. Exhaled nitric oxide in the assessment of asthma. Curr Opin Pulm Med, 2004, 10(1): 31–36.
4 Ma Y, Liu CH, Huang YJ. Exhaled nitric oxide in asthma. Chin J Pediatr, 2005, 43(6): 421–422.[马煜,刘传合,黄永坚. 呼出气体中的一氧化氮检测与哮喘.中华儿科杂志,2005,43(6): 421–422.]
5 Buick JB, Lowry RC. Is a reduction in residual volume a sub–clinical manifestation of hydrogen sulfide intoxication? Am J Ind Med, 2000, 37(3): 296–299.
6 Almeida AF, Guidotti TL. Differential sensitivity of lung and brain to sulfide exposure: A peripheral mechanism for apnea. Toxicol Sci, 1999, 50(2): 287–293.
7 Mirandola P, Gobbi G, Sponzilli I, et al. Exogenous hydrogen sulfide induces functional inhibition and cell death of cytotoxic lymphocytes subsets. J Cell Physiol, 2007, 213(3): 826–833.
8 van Zwieten PA. Hydrogen sulphide: Not only foul smelling, but also pathophysiologically relevant. J Hypertens, 2003, 21(10):1819–1820.
9 Lertratanangkoon K, Scimeca JM, Wei JN. Inhibition of glutathione synthesis with propargylglycine enhances N–acetylmethionine protection and methylation in bromobenzene–treated Syrian hamsters. J Nutr, 1999, 129(3): 649–656.
10 Henderson WR Jr, Tang LO, Chu SJ, et al. A role for cysteinyl leukotrienes in airway remodeling in a mouse asthma model. Am J Respir Crit Care Med, 2002, 165(1): 108–116.
11 Shen HH, Wang SB. Effects of budesonide on airway inflammation and remodeling in the ovalbumin sensitized and challenged mice. Chin J Tuber Respir Dis, 2005, 28: 154–159.[沈华浩,王绍斌. 布地奈德干预对卵白蛋白致敏小鼠抗原激发后气道炎症及气道重塑的影响. 中华结核和呼吸杂志,2005,28:154–159.]
12 Horvath I, Hunt J, Barnes PJ, et al. Exhaled breath condensate: Methodological recommendations and unresolved questions. Eur Respir, 2005, 26: 523–548.
13 Guo ZF, Li Q. Abnormal secretion of mucins in asthma. Chin J Tuber Respir Dis, 2004, 26(2): 135–138.[郭志福,李强. 气道黏蛋白分泌异常与支气管哮喘. 中华结核和呼吸杂志,2004, 26(2): 135–138.]
14 Zuhdi AM, Piazza F, Selby DM, et al. Muc–5/5ac mucin messenger RNA and protein expression is a marker of goblet cell metaplasia in marine airways. Am J Respir Cell Mol Bid, 2000, 22(3): 253.
15 Liu JB, Zhang ZX, Liu YJ. Effects of glucocorticoid on Muc5ac mRNA and protein expression in asthmatic mice lung. J Zhengzhou Univ, 2004, 39(4): 610–613.[刘剑波,张珍祥,刘永健. 地塞米松对哮喘小鼠肺组织Muc5ac mRNA和蛋白表达的影响.郑州大学学报,2004,39(4): 610–613.]
16 Wang TS, Xia ZX, Gao YX. Effects of dexamethasone on protein Muc5ac and substance P in asthmatic Wistar rat lung. Mod Med Health, 2006,22(2): 159–161.[王同生,夏熙郑,高元勋. 地塞米松对哮喘大鼠肺组织Muc5ac蛋白和P物质的影响. 现代医药卫生,2006,22(2):159–161.]
17 Blyth DI, Pedrick MS, Savage B, et al. Induction, duration, and resolution of airway goblet cell hyperplasia in a murine model of atopic asthma: Effect of concurrent infection with respiratory syncytial virus and response to dexamethasone. Am J Respir Cell Mol Bid, 1998, 19(1): 38.
18 Stipanuk MH, Beck PW. Characterization of the enzvmic capaciw forcysine desulphhydmtion in fiver and kidney of the rat. Biochem J, 1982, 206(2): 267–277.
19 Johansen D, Ytrehus K, Baxter GF. Exogenous hydrogen sulfide (H2S) protects against regional myocardial ischemia–reperfusion injury––Evidence for a role of K ATP channels. Basic Res Cardiol, 2006, 101(1): 53–60.
20 Zhu YZ, Wang ZJ, Ho P, et al. Hydrogen sulfide and its possible roles in myocardial ischemia in experimental rats. J Appl Physiol, 2007, 102(2): 261–268.
21 Searcy DG, Lee SH. Sulfur reduction by human erythrocytes. J Exp Zool, 1998, 282(3): 310–312.
22 Chunyu Z, Junbao D, Dingfang B, et al. The regulatory effect of hydrogen sulfide on hypoxic pulmonary hypertension in rats. Biochem Biophys Res Commun, 2003, 302(4): 810–816.
23 Zhao W, Zhang J, Lu Y, et al. The vasorelaxant effect of H(2)S as a novel endogenous gaseous K(ATP) channel opener. EMBO J, 2001, 20(21): 6008–6016.
24 Zhang CY, Du JB, Yan H, et al. Effect of a new gasotransmitter, hydrogen sulfide, on collagen remodeling of pulmonary artery under hypoxia. Chin J Tuber Respir Dis, 2005, 28(7): 448–452.[张春雨,杜军保,闫辉,等. 新型内源性气体信号分子硫化氢对低氧性肺血管胶原重塑的影响. 中华结核和呼吸杂志,2005,28(7):448–452.]
25 Wu R, Yao WZ. The regulatory effect of endogenous hydrogen sulfide on acute asthma. Chin J Tuber Respir Dis, 2007, 30(7): 522–526.[伍蕊,姚婉贞. 硫化氢在大鼠急性支气管哮喘模型中的变化及意义. 中华结核和呼吸杂志,2007,30(7):522–526.]
26 Kimura H. Hydrogen sulfide as a neuromodulator. Mol Neurobiol, 2002, 26(1): 13–19.
27 Eto K, Ogasawara M, Umemura K, et al. Hydrogen sulfide is produced in response to neuronal excitation. J Neurosci, 2002, 22(9): 3386–3391.
28 Geng B, Chang L, Pan C, et al. Endogenous hydrogen sulfide regulation of myocardial injury induced by isoproterenol. Biochem Biophys Res Commun, 2004, 318(3): 756–763.
29 Chen YH, Yao WZ, Geng B, et al. Endogenous hydrogen sulfide in patiens with COPD. Chest, 2005, 128(5): 3205–3211.
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