早期触摸和环境刺激对新生鼠脑神经生长因子及酪氨酸激酶受体A的影响

doi:10.3877/cma.j.issn.1673-5250.2007.03.108

目的

探讨早期触摸和环境刺激对新生鼠额皮质、海马神经生长因子(nerve growth factor，NGF)及酪氨酸激酶受体A(tyrosine kinase receptor A，Trk A)的影响。

方法

60只正常新生鼠被随机分为干预组与对照组。对干预组进行早期触摸14 d，随后置于丰富环境14 d。其NGF含量及Trk A表达，分别采用ELISA和免疫组织化学方法进行检测。

结果

在额皮质和海马，干预组大鼠NGF含量分别为(2343. 58 ±133. 15)pg/g和(1547. 42 ± 65. 32)pg/g，Trk A阳性细胞数分别为(80. 56 ±20. 12)/HF和(66. 42±21. 83)/HF;而对照组NGF含量分别为(2174. 70±165. 21)pg/g和(1475. 23±76. 72)pg/g，Trk A阳性细胞数分别为(63. 50±18. 23)/HF和(50. 45±17. 28)/HF。干预组NGF含量和Trk A阳性细胞数均明显高于对照组(P<0. 05)。

结论

脑组织内NGF的含量和Trk A表达的提高，可能与早期触摸和环境剌激促进脑发育有关。

关键词: 早期触摸, 环境刺激, 神经生长因子, 酪氨酸激酶受体A, 大鼠

Objective

To explore the effects of the neonatal handling and the enriched environment on nerve growth factor (NGF) and its tyrosine kinase receptor A (Trk A) in the forehead cortex and hippocampus of neonatal rats.

Methods

60 neonatal rats were divided into the early intervention group and the control group randomly. The rats in early intervention group received the neonatal handling for14 d and then were kept in an enriched environment for another 14 d. NGF levels were determined by an enzyme-linked immunosorbent assay (ELISA) method and positive the cells expressed Trk A were stained by immunohistochemistry.

Results

In the forehead cortex and hippocampus, NGF levels were(2343. 58±133. 15) pg/g and(1547. 42 ± 65. 32) pg/g in the intervention group, which were much higher than those in the control group [(2174. 70±165. 21) pg/g and(1475. 23 v 76. 72)pg/g] (P<0. 05). The numbers of neurons labelled with Trk A antibody in the forehead cortex and hippocampus of the intervention group were (80. 56 ± 20. 12)cells/HF and(66. 42 ± 21. 83)cells/HF, which were more than those in the control group [(63. 50±18. 23)/HF and(50. 45 ± 17. 28)/HF](P<0. 05).

Conclusion

The neonatal handling and the enriched environment may improve brain development by increasing the levels of endogenetic NGF levels and Trk A expressed in the brain.

Key words: neonatal handling, enriched environment, nerve growth factor (NGF), tyrosine kinase receptor A(Trk A), rats

图1　干预组与对照组海马CA₃区Trk A受体免疫反应神经元

	1　鲍秀兰主编.新生儿行为能力和0〜3岁早期教育.第1版.北京：中国少年儿童出版社，1995，212-219,338-351.
	2　Young D，Lawlor PA，Leone P，et al. Environment enriched inhibits spontaneous apoptosis，prevent seizures and isneuroprotetive. Nat Med,1999,5：448-453.
	3　Philips MF，Mattiasson G, Wieloch T，et al. Neuroprotective and behavioral efficacy of nerve growth factor-transfected hippocampal progenitor cell transplants after experimental traumatic brain injury. Neurosurg，2001，94：765-774.
	4　Takatori M，Kuroda Y，Hirose M. Local anesthetics suppress nerve growth factor-mediated neurite outgrowth by inhibition of tyrosine kinase activity of TrkA. Anesth Analg，2006，102: 462-467.
	5　Meancy MJ, Aitken DH, Berkel CV, et al. Effect of Neonatal handling on age-related impairments associated with the hippocampus. Science,1988，239: 766-768.
	6　Pham TM, Soderstrom S, Henriksson BG, et al. Effects of neonatal stimulation on later cognitive function and hippocampal nerve growth factor. Behav Brain Res, 1997，8:113-120.
	7　Soderstrom S，Hallbook F，Ibanez CF, et al. Recombinant human beta-nerve growth factor ( NGF)：Biological activity and properties in an enzyme immunoassay. Neurosci Res, 1990，27:665-677.
	8　Dekosky ST, Ikonomovic MD, Styren SD. Upregulation of choline acetyltransferase activity in hippocampus and frontal cortex of elderly subjects with mild cognitive impairment. Ann Neurol,2002,51:145-155.
	9　Fagan AM，Garber M，Barbacid M，et al. A role for TrkA during maturation of striatal and basal forebrain cholinergic neurons in vivo. J Neurosci, 1997,17:7644-7654.
	10　Sofroniew MV, Howe CL，Molliam WC. Nerve growth factor signaling, neuroprotection, and neural repair. Annu Rev Neurosci,2001,24：1217-1281.
	11　Thorne RG，Frey WH. Delivery of neurotrophic factors to the central nervous system：Pharmacokinetic considerations. Clin Pharmacokinet，2001,40(12)：907-946.
	12　Wagner AD, Schacter DL, Rotte M, et al. Building memories：Remembering and forgetting of verbal experiences as predicted by brain activity. Science, 1998,281:1188-1191.

[1]	刘思锐, 赵辰阳, 张睿, 张一休, 杨萌. 多普勒超声对孕鼠子宫动脉不同节段血流动力学参数的评估[J/OL]. 中华医学超声杂志（电子版）, 2024, 21(09): 877-883.
[2]	李朋, 苗立帅, 朱智奇. 四氢嘧啶对大鼠关节软骨细胞的保护作用[J/OL]. 中华关节外科杂志(电子版), 2024, 18(01): 69-77.
[3]	张巧梅, 孙小平, 李冠胜, 邓扬嘉. 针灸对大鼠呼吸机相关性肺炎中性粒细胞归巢及胞外诱捕网的影响[J/OL]. 中华危重症医学杂志(电子版), 2023, 16(04): 265-271.
[4]	林琳, 田思萌, 于永华, 徐飞飞, 黄明莉. 干细胞及其外泌体治疗宫腔黏连的研究现状[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(03): 271-275.
[5]	邓健, 王少华, 陈尊, 邹振庄. Keap1/Nrf2信号通路在脂多糖诱导宫内感染致新生鼠支气管肺发育不良的作用机制[J/OL]. 中华妇幼临床医学杂志(电子版), 2023, 19(06): 665-674.
[6]	张晓燕, 肖东琼, 高沪, 陈琳, 唐发娟, 李熙鸿. 转录因子12过表达对脓毒症相关性脑病大鼠大脑皮质的保护作用及其机制[J/OL]. 中华妇幼临床医学杂志(电子版), 2023, 19(05): 540-549.
[7]	靳茜雅, 黄晓松, 谭诚, 蒋琴, 侯昉, 李瑶悦, 徐冰, 贾红慧, 刘文英. 产前他克莫司治疗对先天性膈疝大鼠病理模型肺血管重构的影响[J/OL]. 中华妇幼临床医学杂志(电子版), 2023, 19(04): 428-436.
[8]	杨文飞, 郝嘉文, 鲁梦远, 赵学刚, 李聪颖, 盖晨阳, 张晶, 张庆富. 高压电烧伤对大鼠心肌氧化应激的影响及N-乙酰半胱氨酸的干预作用[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(02): 106-112.
[9]	魏强, 张明祥, 陈强谱, 孙宝房. 增味小承气汤对梗阻性黄疸大鼠胃肠道动力的影响[J/OL]. 中华普通外科学文献(电子版), 2023, 17(04): 267-270.
[10]	张礼刚, 邹志辉, 许顺, 蔡可可, 胡永涛, 梁朝朝. 酒精对慢性非细菌性前列腺炎中T淋巴细胞变化的影响研究[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(01): 74-81.
[11]	赛甫丁·艾比布拉, 买买提·依斯热依力, 李义亮, 王永康, 王志, 克力木·阿不都热依木. 不同材质补片修补对腹壁疝大鼠腹横筋膜组织转化生长因子-β1及Collagen合成代谢的作用[J/OL]. 中华疝和腹壁外科杂志(电子版), 2024, 18(02): 161-167.
[12]	刘锦程, 王斌, 张雯, 张明周, 刘禹, 叶东樊, 黄赞胜, 邱凌霄, 卿斌, 王创业, 王南博, 王苹, 郭宇航, 周培花, 程秋霞, 徐智. 肺泡灌洗液RASSF1A及SHOX2甲基化联合径向超声特征对肺结节性质鉴别诊断的意义[J/OL]. 中华肺部疾病杂志(电子版), 2024, 17(04): 505-511.
[13]	杨蕴钊, 周诚, 石美涵, 赵静, 白雪源. 人羊水间充质干细胞对膜性肾病大鼠的治疗作用[J/OL]. 中华肾病研究电子杂志, 2023, 12(04): 181-186.
[14]	萨仁高娃, 张英霞, 邓伟, 闫诺, 樊宁. 超声引导下鼠肝消融术后组织病理特征的变化规律及影响[J/OL]. 中华消化病与影像杂志(电子版), 2023, 13(06): 394-398.
[15]	王小红, 钱晶, 翁文俊, 周国雄, 朱顺星, 祁小鸣, 刘春, 王萍, 沈伟, 程睿智, 秦璟灏. 巯基丙酮酸硫基转移酶调控核因子κB信号介导自噬对重症急性胰腺炎大鼠的影响及机制[J/OL]. 中华消化病与影像杂志(电子版), 2023, 13(06): 422-426.

阅读次数

全文

摘要

选择文件类型/文献管理软件名称

选择包含的内容

Effects of the neonatal handling and the enriched environment on nerve growth factor and its receptor Trk A in the brain of neonatal rats

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

Effects of the neonatal handling and the enriched environment on nerve growth factor and its receptor Trk A in the brain of neonatal rats