早期触摸和环境刺激对新生鼠脑神经生长因子及酪氨酸激酶受体A的影响

doi:10.3877/cma.j.issn.1673-5250.2007.03.108

目的

探讨早期触摸和环境刺激对新生鼠额皮质、海马神经生长因子(nerve growth factor，NGF)及酪氨酸激酶受体A(tyrosine kinase receptor A，Trk A)的影响。

方法

60只正常新生鼠被随机分为干预组与对照组。对干预组进行早期触摸14 d，随后置于丰富环境14 d。其NGF含量及Trk A表达，分别采用ELISA和免疫组织化学方法进行检测。

结果

在额皮质和海马，干预组大鼠NGF含量分别为(2343. 58 ±133. 15)pg/g和(1547. 42 ± 65. 32)pg/g，Trk A阳性细胞数分别为(80. 56 ±20. 12)/HF和(66. 42±21. 83)/HF;而对照组NGF含量分别为(2174. 70±165. 21)pg/g和(1475. 23±76. 72)pg/g，Trk A阳性细胞数分别为(63. 50±18. 23)/HF和(50. 45±17. 28)/HF。干预组NGF含量和Trk A阳性细胞数均明显高于对照组(P<0. 05)。

结论

脑组织内NGF的含量和Trk A表达的提高，可能与早期触摸和环境剌激促进脑发育有关。

关键词: 早期触摸, 环境刺激, 神经生长因子, 酪氨酸激酶受体A, 大鼠

Objective

To explore the effects of the neonatal handling and the enriched environment on nerve growth factor (NGF) and its tyrosine kinase receptor A (Trk A) in the forehead cortex and hippocampus of neonatal rats.

Methods

60 neonatal rats were divided into the early intervention group and the control group randomly. The rats in early intervention group received the neonatal handling for14 d and then were kept in an enriched environment for another 14 d. NGF levels were determined by an enzyme-linked immunosorbent assay (ELISA) method and positive the cells expressed Trk A were stained by immunohistochemistry.

Results

In the forehead cortex and hippocampus, NGF levels were(2343. 58±133. 15) pg/g and(1547. 42 ± 65. 32) pg/g in the intervention group, which were much higher than those in the control group [(2174. 70±165. 21) pg/g and(1475. 23 v 76. 72)pg/g] (P<0. 05). The numbers of neurons labelled with Trk A antibody in the forehead cortex and hippocampus of the intervention group were (80. 56 ± 20. 12)cells/HF and(66. 42 ± 21. 83)cells/HF, which were more than those in the control group [(63. 50±18. 23)/HF and(50. 45 ± 17. 28)/HF](P<0. 05).

Conclusion

The neonatal handling and the enriched environment may improve brain development by increasing the levels of endogenetic NGF levels and Trk A expressed in the brain.

Key words: neonatal handling, enriched environment, nerve growth factor (NGF), tyrosine kinase receptor A(Trk A), rats

图1　干预组与对照组海马CA₃区Trk A受体免疫反应神经元

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Effects of the neonatal handling and the enriched environment on nerve growth factor and its receptor Trk A in the brain of neonatal rats

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