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中华妇幼临床医学杂志(电子版)

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2020, Vol. 16 ›› Issue (06): 627 -633. doi: 10.3877/cma.j.issn.1673-5250.2020.06.002

Special Issue: ；

Forum

Current researches status on proteomics in congenital heart disease

Jing He1, Hanmin Liu1,†()   

  1. 1. Department of Pediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2020-08-11 Revised:2020-11-17 Published:2020-12-01
  • Corresponding author: Hanmin Liu
  • Supported by:
    National Natural Science Foundation of China(81673257); National Program on Key Basic Research Project (973 Program)(2017CB511905)
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With accumulation of large numbers of DNA sequences in databases, researchers have become increasingly aware that the complete genome sequence and transcriptional changes of thousands of genes in patients with cardiovascular disease alone are not sufficient to elucidate the pathogenesis of cardiovascular disease from a molecular perspective.The presence of an open reading frame in a genome sequence does not necessarily imply the presence of a functional gene. With the advent of the postgenome era, proteomics as a large-scale, high-flux, systematic emerging discipline, researches a particular type of all proteins in cells, tissues or body fluids through a variety of technologies, aims to analyze dynamic changes of protein expression in cells and modification status, understand the interaction between proteins in cells, and thus reveal protein function and cell life activities.Proteomics specializes in study of biological and pathophysiological issues from the perspective of proteins. Congenital heart disease (CHD) is the leading birth defect in China and one of the main causes of infants death and CHD-related complications, the early diagnosis and intervention of CHD is the key to CHD, ultrasound is a common screening method for CHD in clinical practice, but is not suitable for early screening of CHD, proteomics can map all proteins expressed in heart at any time and under any conditions, and determine the protein changes related to etiology, progression, outcome and treatment response of CHD through analysis of differential expressed proteins. The identification of these CHD-related specific proteins and to study their expression patterns, post-translational modification status and functional characteristics are the basis and key to etiology and pathophysiology of various types of CHD.This article focus on research status of technology and classification of proteomics, advantages and application of proteomics in diagnosis, treatment and prognosis evaluation of CHD, and the future prospects and prospects of proteomics.

Key words: Proteomics, Congenital heart disease, Plasma proteome, Proteins, Proteomics methodology, Fluids and secretions, Biological markers
[1]
Wilkins MR, Sanchez JC, Gooley AA, et al. Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it[J]．Biotechnol Genet Eng Rev，1996，13:19-50. DOI：10.1080/02648725.1996.10647923.
[2]
Wasinger VC, Cordwell SJ, Cerpa-Poljak A, et al. Progress with gene-product mapping of the Mollicutes: genitalium[J]. Electrophoresis，1995，16(7):1090-1094. DOI：10.1002/elps.11501601185.
[3]
Wu Y, Zhang J, Wang M, et al. Proteomics analysis indicated the protein expression pattern related to the development of fetal conotruncal defects[J]．J Cell Physiol, 2019, 234(8): 13544-13556. DOI: 10.1002/jcp.28033.
[4]
Xu C, Su X, Chen Y, et al. Proteomics analysis of plasma protein changes in patent ductus arteriosus patients[J]．Ital J Pediatr，2020，46(1):64-70. DOI：10.1186/s13052-020-00831-6.
[5]
景洪标，丁华野，丁彦青．蛋白质组学及其在乳腺癌研究中的应用[J]．临床与实验病理学杂志，2005，21(1):110-111.DOI：10.3969/j.issn.1001-7399.2005.01.029.
[6]
Faber MJ, Agnetti G, Bezstarosti K, et al. Recent developments in proteomics: implications for the study of cardiac hypertrophy and failure[J]．Cell Biochem Biophys, 2006，44(1):11-29. DOI：10.1385/CBB:44:1:011.
[7]
Gowda M, Thiagarajan M, Satheesh S, et al. Prenatal grading of fetal congenital heart disease and its influence on decision making during pregnancy and postnatal period: a prospective study[J]．J Matern Fetal Neonatal Med, 2020: 1-9.DOI: 10.1080/14767058.2020.1814245.
[8]
Hepponstall M, Konstantinov IE. Proteomics in paediatric cardiac surgery: is a personalised approach feasible？[J]．Proteomics Clin Appl，2014，8(11-12):851-861.DOI：10.1002/prca.201400054.
[9]
郁夏风，刘锦纷．蛋自质组学与先天性心脏病形成机制的研究现状[J]．临床儿科杂志，2011，29(7):696-698.DOI：10.3969/j.issn.1000-3606.2011.07.025.
[10]
Banks RE, Dunn MJ, Hochstrasser DF, et al. Proteomics: new perspectives, new biomedical opportunities[J]．Lancet，2000，356(9243):1749-1756. DOI: 10.1016/S0140-6736(00)03214-1.
[11]
Macri J, Rapundalo ST. Application of proteomics to the study of cardiovascular biology[J]. Trends Cardiovasc Med，2001，11(2): 66-75. DOI: 10.1016/s1050-1738(01)00088-3.
[12]
Dunn MJ. Studying heart disease using the proteomic approach[J]. Drug Discov Today, 2000, 5(2): 76-84. DOI：10.1016/s1359-6446(99)01449-x.
[13]
Slagle CE, Conlon FL. Emerging field of cardiomics: high-throughput investigations into transcriptional regulation of cardiovascular development and disease[J]. Trends Genet，2016, 32(11): 707-716. DOI: 10.1016/j.tig.2016.09.002.
[14]
Jung E, Heller M, Sanchez JC, et al. Proteomics meets cell biology: the establishment of subcellular proteomes[J]. Electrophoresis, 2000，21(16):3369-3377. DOI：10.1002/1522-2683(20001001)21:16<3369::AID-ELPS3369>3.0.CO;2-7.
[15]
Zhang T, Yuan Q, Gu Z, et al. Advances of proteomics technologies for multidrug-resistant mechanisms[J]. Future Med Chem，2019，11(19):2573-2593. DOI：10.4155/fmc-2018-0507.
[16]
Graves PR, Haystead TA. Molecular biologist′s guide to proteomics[J]. Microbiol Mol Biol Rev，2002，66(1):39-63. DOI：10.1128/mmbr.66.1.39-63.2002.
[17]
Meleady P. Two-dimensional gel electrophoresis and 2D-DIGE[J]. Methods Mol Biol，2018，1664:3-14. DOI：10.1007/978-1-4939-7268-5_1.
[18]
Westermeier R, Marouga R. Protein detection methods in proteomics research[J]. Biosci Rep，2005，25(1-2):19-32. DOI：10.1007/s10540-005-2845-1.
[19]
Loscalzo J. Proteomics in cardiovascular biology and medicine[J]. Circulation，2003，108(4):380-383. DOI：10.1161/01.CIR.0000079867.56212.17.
[20]
Boutet E, Lieberherr D, Tognolli M, et al. UniProtKB/Swiss-Prot, the manually annotated section of the Uniprot knowledgebase: how to use the entry view[J]. Methods Mol Biol, 2016，1374:23-54. DOI：10.1007/978-1-4939-3167-5-2.
[21]
Wu WW, Wang G, Baek SJ, et al. Comparative study of three proteomic quantitative methods, DIGE, cICAT, and iTRAQ, using 2D gel- or LC-MALDI TOF/TOF[J]. J Proteome Res, 2006，5(3):651-658. DOI：10.1021/pr050405o.
[22]
Issaq HJ, Chan KC, Janini GM, et al. Multidimensional separation of peptides for effective proteomic analysis[J]. J Chromatogr B Analyt Technol Biomed Life Sci，2005，817(1):35-47. DOI：10.1016/j.jchromb.2004.07.042.
[23]
Martyniuk CJ, Alvarez S, Denslow ND. DIGE and iTRAQ as biomarker discovery tools in aquatic toxicology[J]. Ecotoxicol Environ Saf，2012，76(2):3-10. DOI：10.1016/j.ecoenv.2011.09.020.
[24]
Gan CS, Chong PK, Pham TK, et al. Technical, experimental, and biological variations in isobaric tags for relative and absolute quantitation (iTRAQ)[J]. J Proteome Res, 2007，6(2):821-827. DOI：10.1021/pr060474i.
[25]
张海洋，牛春雨，赵自刚．血浆蛋白组学技术及其应用进展[J]．中国微循环，2009，13(3):207-210.
[26]
Anderson NL, Anderson NG. The human plasma proteome: history, character, and diagnostic prospects[J]. Mol Cell Proteomics，2002，1(11):845-867. DOI：10.1074/mcp.r200007-mcp200.
[27]
Anderson NL, Polanski M, Pieper R, et al. The human plasma proteome: a nonredundant list developed by combination of four separate sources[J]. Mol Cell Proteomics，2004，3(4):311-326. DOI：10.1074/mcp.M300127-MCP200.
[28]
Geho DH, Liotta LA, Petricoin EF, et al. The amplified peptidome: the new treasure chest of candidate biomarkers[J]. Curr Opin Chem Biol，2006，10(1):50-55. DOI：10.1016/j.cbpa.2006.01.008.
[29]
Petricoin EF, Ardekani AM, Hitt BA, et al. Use of proteomic patterns in serum to identify ovarian cancer[J]. Lancet，2002，359(9306):572-577.DOI：10.1016/S0140-6736(02)07746-2.
[30]
Granger J, Siddiqui J, Copeland S, et al. Albumin depletion of human plasma also removes low abundance proteins including the cytokines[J]. Proteomics，2005，5(18):4713-4718. DOI：10.1002/pmic.200401331.
[31]
Jäger D, Jungblut PR, Müller-Werdan U. Separation and identification of human heart proteins[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2002，771(1-2):131-153. DOI：10.1016/s1570-0232(02)00039-9.
[32]
Baker CS, Corbett JM, May AJ, et al. A human myocardial two-dimensional electrophoresis database: protein characterisation by microsequencing and immunoblotting[J]. Electrophoresis, 1992，13(9-10):723-726. DOI：10.1002/elps.11501301154.
[33]
Venter JC, Adams MD, Myers EW, et al. The sequence of the human genome[J]. Science，2001，291(5507):1304-1351. DOI：10.1126/science.105804.
[34]
Jungblut P, Otto A, Regitz V, et al. Identification of human myocard proteins separated by two-dimensional electrophoresis[J]. Electrophoresis, 1992，13(9-10):739-741. DOI：10.1002/elps.11501301159.
[35]
Kovalyov LI, Shishkin SS, Efimochkin AS, et al. The major protein expression profile and two-dimensional protein database of human heart[J]. Electrophoresis, 1995，16(7):1160-1169. DOI：10.1002/elps.11501601192.
[36]
Pleissner KP, Söding P, Sander S, et al. Dilated cardiomyopathy-associated proteins and their presentation in a WWW-accessible two-dimensional gel protein database[J]. Electrophoresis，1997，18(5):802-808. DOI：10.1002/elps.1150180525.
[37]
Chen L, Guan J, Wei Q, et al. Potential role of " omics" technique in prenatal diagnosis of congenital heart defects[J]．Clin Chim Acta，2018，482(1):185-190. DOI：10.1016/j.cca.2018.04.011.
[38]
Koster MP, Pennings JL, Imholz S, et al. Proteomics and Down syndrome screening: a validation study[J]. Prenat Diagn，2010，30(11):1039-1043. DOI：10.1002/pd.2606.
[39]
Auer J, Camoin L, Guillonneau F, et al. Serum profile in preeclampsia and intra-uterine growth restriction revealed by iTRAQ technology[J]. J Proteomics，2010，73(5):1004-1017. DOI：10.1016/j.jprot.2009.12.014.
[40]
An D, Wei X, Li H, et al. Identification of PCSK9 as a novel serum biomarker for the prenatal diagnosis of neural tube defects using iTRAQ quantitative proteomics[J]. Sci Rep, 2015，5:17559. DOI：10.1038/srep17559.
[41]
Chen L, Gu H, Li J, et al. Comprehensive maternal serum proteomics identifies the cytoskeletal proteins as non-invasive biomarkers in prenatal diagnosis of congenital heart defects[J]. Sci Rep, 2016，6:19248. DOI：10.1038/srep19248.
[42]
Zhang X, Sun Y, Zhu J, et al. Epidemiology, prenatal diagnosis, and neonatal outcomes of congenital heart defects in eastern China: a hospital-based multicenter study[J]. BMC Pediatr, 2020，20(1):416. DOI：10.1186/s12887-020-02313-4.
[43]
Zhu H, Kartiko S, Finnell RH. Importance of gene-environment interactions in the etiology of selected birth defects[J]. Clin Genet，2009，75(5):409-423. DOI：10.1111/j.1399-0004.2009.01174.x.
[44]
Nath AK, Krauthammer M, Li P, et al. Proteomic-based detection of a protein cluster dysregulated during cardiovascular development identifies biomarkers of congenital heart defects[J]. PLoS One，2009，4(1):e4221. DOI：10.1371/journal.pone.0004221.
[45]
Chigusa S, Moroi T, Shoji Y. State-of-the-art calculation of the decay rate of electroweak vacuum in the standard model[J]. Phys Rev Lett，2017，119(21):211801. DOI：10.1103/PhysRevLett.119.211801.
[46]
Dorr KM, Conlon FL. Proteomic-based approaches to cardiac development and disease[J]. Curr Opin Chem Biol，2019，48:150-157. DOI：10.1016/j.cbpa.2019.01.001.
[47]
Xuan C, Gao G, Yang Q, et al. Proteomic study reveals plasma protein changes in congenital heart diseases[J]. Ann Thorac Surg，2014，97(4):1414-1419. DOI：10.1016/j.athoracsur.2013.11.069.
[48]
Witke W, Sharpe AH, Hartwig JH, et al. Hemostatic, inflammatory, and fibroblast responses are blunted in mice lacking gelsolin[J]．Cell，1995，81(1):41-51. DOI：10.1016/0092-8674(95)90369-0.
[49]
Tempe DK, Virmani S. Coagulation abnormalities in patients with cyanotic congenital heart disease[J]. J Cardiothorac Vasc Anesth，2002，16(6):752-765. DOI：10.1053/jcan.2002.128436.
[50]
Munthe-Fog L, Hummelshøj T, Honoré C, et al. Immunodeficiency associated with FCN3 mutation and ficolin-3 deficiency[J]. N Engl J Med，2009，360(25):2637-2644. DOI：10.1056/NEJMoa0900381.
[51]
Huang Q, Geng Z, Chen T, et al. Comparative proteomic analysis of plasma of children with congenital heart disease[J]. Electrophoresis, 2019，40(14):1848-1854. DOI：10.1002/elps.201900098.
[52]
Vekich JA, Belmont PJ, Thuerauf DJ, et al. Protein disulfide isomerase-associated 6 is an ATF6-inducible ER stress response protein that protects cardiac myocytes from ischemia/reperfusion-mediated cell death[J]. J Mol Cell Cardiol，2012，53(2):259-267. DOI：10.1016/j.yjmcc.2012.05.005.
[53]
Groenendyk J, Peng Z, Dudek E, et al. Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis[J]. Sci Signal，2014，7(329):ra54. DOI：10.1126/scisignal.2004983.
[54]
Prins D, Michalak M. Endoplasmic reticulum proteins in cardiac development and dysfunction[J]. Can J Physiol Pharmacol，2009，87(6):419-425. DOI：10.1139/y09-032.
[55]
Tumani H, Lehmensiek V, Lehnert S, et al. 2D DIGE of the cerebrospinal fluid proteome in neurological diseases[J]. Expert Rev Proteomics，2010，7(1):29-38. DOI：10.1586/epr.09.99.
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