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Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2020, Vol. 16 ›› Issue (06): 627 -633. doi: 10.3877/cma.j.issn.1673-5250.2020.06.002

Special Issue: ；

Forum

Current researches status on proteomics in congenital heart disease

Jing He1, Hanmin Liu1,†()   

  1. 1. Department of Pediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2020-08-11 Revised:2020-11-17 Published:2020-12-01
  • Corresponding author: Hanmin Liu
  • Supported by:
    National Natural Science Foundation of China(81673257); National Program on Key Basic Research Project (973 Program)(2017CB511905)
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With accumulation of large numbers of DNA sequences in databases, researchers have become increasingly aware that the complete genome sequence and transcriptional changes of thousands of genes in patients with cardiovascular disease alone are not sufficient to elucidate the pathogenesis of cardiovascular disease from a molecular perspective.The presence of an open reading frame in a genome sequence does not necessarily imply the presence of a functional gene. With the advent of the postgenome era, proteomics as a large-scale, high-flux, systematic emerging discipline, researches a particular type of all proteins in cells, tissues or body fluids through a variety of technologies, aims to analyze dynamic changes of protein expression in cells and modification status, understand the interaction between proteins in cells, and thus reveal protein function and cell life activities.Proteomics specializes in study of biological and pathophysiological issues from the perspective of proteins. Congenital heart disease (CHD) is the leading birth defect in China and one of the main causes of infants death and CHD-related complications, the early diagnosis and intervention of CHD is the key to CHD, ultrasound is a common screening method for CHD in clinical practice, but is not suitable for early screening of CHD, proteomics can map all proteins expressed in heart at any time and under any conditions, and determine the protein changes related to etiology, progression, outcome and treatment response of CHD through analysis of differential expressed proteins. The identification of these CHD-related specific proteins and to study their expression patterns, post-translational modification status and functional characteristics are the basis and key to etiology and pathophysiology of various types of CHD.This article focus on research status of technology and classification of proteomics, advantages and application of proteomics in diagnosis, treatment and prognosis evaluation of CHD, and the future prospects and prospects of proteomics.

Key words: Proteomics, Congenital heart disease, Plasma proteome, Proteins, Proteomics methodology, Fluids and secretions, Biological markers
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