Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2017, Vol. 13 ›› Issue (02): 189 -193. doi: 10.3877/cma.j.issn.1673-5250.2017.02.012

Special Issue:

Original Article

Correlation on the S100B protein, caspase-3 protein levels of neonatal umbilical cord blood and neonatal behavior neurological assessment score in gestational diabetes mellitus women

Wei Fan1, Li Hao1, Qigai Yin1,(), Shan Li1   

  1. 1. Department of Neonatal, First People′s Hospital of Lianyungang, Lianyungang 222000, Jiangsu Province, China
  • Received:2016-12-04 Revised:2017-03-01 Published:2017-04-01
  • Corresponding author: Qigai Yin
  • About author:
    Corresponding author: Yin Qigai, Email:
Objective

To discuss correlation on the S100B protein, caspase-3 protein levels of neonatal umbilical cord blood and neonatal behavior neurological assessment (NBNA) score in gestational diabetes mellitus (GDM) women.

Methods

From January 2015 to March 2016, a total of 86 neonates delivered in the First People′s Hospital of Lianyungang were recruited into this study. They were divided into GDM group (n=33) and control group (n=53) on the basis of mother with or without GDM during pregnancy period. The content of S100B protein and caspase-3 protein of neonatal umbilical cord blood were detected by enzyme-linked immuosorbent assay (ELISA). The NBNA scores were determined at postnatal day 3.

Results

①There were no significant differences between two groups in the aspects of constitution ratio of gender, gestational age, birth weight maternal age, etc. (P>0.05). ②The contents of S100B protein and caspase-3 protein in GDM group were higher than those of control group [(38.8±9.3) pg/mL vs (12.4±2.6) pg/mL, (5.3±1.7) μg/mL vs (2.8±0.6) μg/mL], and the differences were statistically significant (t=8.314, 15.977; P<0.001). The NBNA score in GDM group was lower than that of control group [(36.3±1.2) scores vs (38.3±1.2) scores], and the difference was statistically significant (t=8.129, P<0.001). ③In GDM group, the content of S100B protein and caspase-3 protein of neonatal umbilical cord blood were negative correlated with NBNA score in GDM group, respectively (r=-0.583, -0.814; P<0.001), and the content of S100B protein of neonatal umbilical cord blood was positive correlated with caspase-3 protein (r= 0.394, P=0.023 ). While in control group, S100B protein, caspase-3 protein of neonatal umbilical cord blood and NBNA score had no correlation with each other (P>0.05).

Conclusions

GDM is probably the one of the risk factors of neonatal brain damage. It is suggested that S100B protein and caspase-3 protein of neonatal umbilical cord blood can be used as an reference index predicting the degree of brain damage.

表1 2组新生儿一般临床资料比较
表2 2组新生儿脐血血清S100B蛋白、caspase-3蛋白含量及NBNA评分比较(±s)
[1]
Hawdon JM. Babies born after diabetes in pregnancy: what are the short- and long-term risks and how can we minimise them? [J]. Best Pract Res Clin Obstet Gynaecol, 2011, 25(1): 91-104.
[2]
中华医学会妇产科学分会产科学组,中华医学会围产医学分会妊娠合并糖尿病协作组. 妊娠合并糖尿病诊治指南(2014)[J]. 中华妇产科杂志, 2014, 49(8): 561-569.
[3]
Allore R, O′Hanlon D, Price R, et al. Gene encoding the beta subunit of S100 protein is on chromosome 21: implications for Down syndrome [J]. Science, 1988, 239(4845): 1311-1313.
[4]
Savard A, Brochu ME, Chevin M, et al. Neuronal self-injury mediated by IL-1β and MMP-9 in a cerebral palsy model of severe neonatal encephalopathy induced by immune activation plus hypoxia-ischemia [J]. J Neuroinflammat, 2015, 12: 111.
[5]
Donato R, Sorci G, Riuzzi F, et al. S100B′s double life: intracellular regulator and extracellular signal [J]. Biochim Biophys Acta, 2009, 1793(6): 1008-1022.
[6]
Terada T. Recurrent multifocal langerhans cell histiocytosis of the mandible and maxilla in a 46-year-old man: a pathologic case report [J]. Int J Clin Exp Pathol, 2013, 6(5): 939-942.
[7]
陈珊,李薇,瞿柳红,等. 血浆S100B蛋白和髓鞘碱性蛋白与早产儿脑损伤的关系[J]. 中华实用儿科杂志,2014, 29(12): 902-906.
[8]
荣昆,高缨.妊娠期糖尿病对围生期新生儿神经行为发育的影响[J].中国妇幼保健,2015,30(20):3353-3355.
[9]
Samejima K, Svingen PA, Basi GS, et al. Caspase-mediated cleavage of DNA topoisomerase I at unconventional sites during apoptosis [J]. J Biol Chem, 1999, 274(7): 4335-4340.
[10]
Nicholson DW. Caspase structure, proteolytic substrates, and function during apoptotic cell death [J]. Cell Death Differ, 1999, 6(11): 1028-1042.
[11]
汪皓,崔睿.预吸氧对新生鼠对宫内再灌注损伤caspase-3和iNOS的影响[J].中国妇幼保健,2013,28(45):2782-2783.
[12]
杜雪平,李天慧.新生儿缺氧缺血性脑病血清中半胱氨酸蛋白酶-3测定的意义[J].中国实用神经疾病杂志,2014,17(6):79-82.
[13]
Byrne E, Campbell SK. Physical therapy observation and assessment in the neonatal intensive care unit [J]. Phys Occup Ther Pediatr, 2013, 33(1): 39-74.
[14]
Voigt B, Brandl A, Pietz J, et al. Negative reactivity in toddlers born prematurely: indirect and moderated pathways considering self-regulation, neonatal distress and parenting stress [J]. Infant Behav Dev, 2013, 36(1): 124-138.
[15]
陈丽琼,沈剑. 妊娠期糖尿病对新生儿神经行为发育影响的Meta分析[J].现代实用医学杂志,2012, 24(2): 221.
[16]
罗淑静,杨慧霞.晚期糖基化终末产物及其受体在妊娠期糖尿病孕鼠的子鼠脑损伤中的作用[J]. 中华妇产科杂志,2012, 47(5):364-367.
[17]
Perna R, Loughan AR, Le J, et al. Gestational diabetes: long-term central nervous system developmental and cognitive sequelae [J]. Appl Neuropsychol Child, 2015, 4(3): 217-220.
[18]
Roberts AL, Nguyen VT. Growing evidence that maternal gestational diabetes increases risk of autism in offspring [J]. Evid Based Ment Health, 2015, 18(4): 113.
[19]
Xiang AH, Wang X, Martinez MP, et al. Association of maternal diabetes with autism in offspring [J]. JAMA, 313(14): 1425-1434.
[20]
Teramo K, Klemetti M, Tikkanen M, et al. Maternal diabetes and fetal hypoxia [J]. Duodecim, 2013, 129(3): 228-234.
[21]
Del Rio D, Stewart AJ, Pellegrini N. et al. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress [J]. Nutr Metab Cardiovasc Dis, 2005, 15(4): 316-328.
[22]
Myatt L, Cui X. Oxidative stress in the placenta [J]. Histochem Cell Biol, 2004, 122(4): 369-382.
[23]
Ahmed A. New insights into the etiology of preeclampsia: identification of key elusive factors for the vascular complications [J]. Thromb Res, 2011, 127(Suppl 3): S72-S75.
[24]
Myatt L, Kossenjans W, Sahay R, et al. Oxidative stress causes vascular dysfunction in the placenta [J]. J Matern Fetal Med, 2000, 9(1): 79-82.
[25]
丰有吉.妇产科学.2版[M]. 北京:人民卫生出版社,2010:91.
[26]
Hollander MH, Paarlberg KM, Huisjes AJ. Gestational diabetes: a review of the current literature and guidelines [J]. Obstet Gynecol Surv, 2007, 62(2): 125-136.
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