Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2011, Vol. 07 ›› Issue (03): 227 -230. doi: 10.3877/cma.j.issn.1673-5250.2011.03.011

Original Article

Methylation of DAPK1 Gene Promoter's CpG Island and Its Role in the Pathogenic Mechanism of Leukemia

Jun-hua LI, Fei ZHAN, Ming CHEN   

  1. Department of Pediatrics, Xiangyang Centre Hospital, Xiangyang 441021, Hubei Province, China
  • Published:2011-06-01
Objective

To study the methylation status of death-associated protein kinase 1 (DAPK1) gene's promoter and its role in the pathogenic mechanism of leukemia.

Methods

DNA and RNA were extracted from a healthy child's peripheral blood mononuclear cells (PBMC) and leukemia cell line HL-60 and were amplified with bisulfite sequencing PCR (BSP) and reverse transcription PCR (RT-PCR), respectively. Methylation status and mRNA expression of DAPK1 gene were compared.

Results

Promoter of DAPK1 gene in healthy child's peripheral blood mononuclear cells displayed unmethylated or low methylated status with normal mRNA expression (methylation rate ≤10%), while it was highly methylated in leukemia cell line HL-60 with no mRNA expression (methylation rate 60%~90%).

Conclusion

Promoter of DAPK1 gene in leukemia cell line HL-60 was hypermethylated. It may correlate with pathogenic mechanism of leukemia.

图1 正常健康儿童外周血单个核细胞DAPK1基因启动子CpG岛甲基化状态
图2 白血病细胞株HL-60的DAPK1基因启动子CpG岛甲基化状态
图3 两组样本DAPK1基因mRNA表达结果
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