The differential gene expression profile in cervical cancer that infected with human papillomavirus 16 screened out by cDNA microarray

doi:10.3877/cma.j.issn.1673-5250.2006.02.106

Objective

To indentify a set of genes related to human papillomavirus 16(HPV 16)of cervical cancer and to discuss the pathogenesis of cervical cancer.

Methods

3 women of cervical cancer infected with human papillomavirus 16 were seclected by Hybrid capture Ⅱ. The human gene expression of cervical cancer and normal cervical tissues were analyzed with 14 112 human genes of cDNA microarray.

Results

244 differential genes expressiond in cervical cancer were screened out. Among them, 136 were up-regulated, 108 were down-regulated. These genes belongs to signal transduction, metabolism, immunity response, protein biosynthesis and ESTs.

Conclusion

Cervical cancer induced by HPV16 is a result of coeffected by polygene and multiple factors. These genes reticulate regulated eachother, direct or indirect in progression to cervical cancer, and cDNA microarray technique is effective in screening out the differentially expressioned genes, and further investigation and functional characterization of the dentified genes is needed to clarify the accurate pathogenesis of cervical cancer.

Key words: cervical carcinoma, human papillomavirus, gene expression profile, cDNA microarray

图1　RNA琼脂糖凝胶电泳

图2　芯片杂交结果。黄色点：代表该基因表达在两种组织中接近；红色点：代表该基因在宫颈正常组织中高表达，而癌组织中低表达或不表达；绿色点：代表该基因在宫颈癌组织中高表达，而正常组织低表达或不表达

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