探讨CD₁₁₇和CD₃₄在子宫腺肌病病灶组织中的分布及CD₁₁₇^＋细胞的数量，从而探讨CD₁₁₇细胞与子宫腺肌病关系。

选择2014年7月至2016年12月在哈尔滨医科大学附属第一医院因子宫腺肌病接受子宫切除术的54例患者的子宫腺肌病病灶组织标本为研究对象，并纳入子宫腺肌病组。选择同期于本院因子宫肌瘤或者卵巢良性肿瘤行子宫切除术的50例患者的正常子宫肌层组织纳入对照组(n＝54)。通过免疫组织化学染色对比研究CD₁₁₇在子宫腺肌病病灶组织和正常子宫肌层组织中的表达水平；通过CD₁₁₇、CD₃₄免疫荧光染色探讨子宫腺肌病病灶组织CD₁₁₇细胞与血管形成的关系。本研究遵循的程序符合哈尔滨医科大学附属第一医院人体试验委员会制定的伦理学标准，得到该委员会批准。本研究征得受试对象的知情同意，并与之签署临床研究知情同意书。

①子宫腺肌病组的病灶组织和对照组的正常子宫肌层组织中均可见CD₁₁₇细胞，CD₁₁₇细胞在子宫腺肌病组病灶组织中的表达水平高于对照组；光学显微镜下子宫腺肌病组平均每个视野的CD₁₁₇细胞计数为(18±4)个，对照组为(8±3)个，二者比较，差异有统计学意义(t＝2.571，P<0.05)。②子宫腺肌病病灶组织CD₃₄、CD₁₁₇免疫荧光双染染色结果显示，荧光显微镜下观察CD₁₁₇^＋细胞为CD₁₁₇^＋/CD₃₄^－细胞。子宫腺肌病病灶组织中存在较多CD₃₄^＋微小血管结构，CD₁₁₇细胞则大致分布于子宫腺肌病病灶的微小血管周围，血管壁的内皮细胞中可见CD₃₄^＋，无CD₁₁₇^＋表达。

CD₁₁₇^＋细胞可能参与子宫腺肌病的发病机制。

To investigate the relationship between the distribution and quantity of CD₁₁₇ in the disease tissue of adenomyosis and angiogenesis, to explore the relationship between CD₁₁₇ cells and adenomyosis.

From July 2014 to December 2016 in the First Affiliated Hospital of Harbin Medical University, gynecological patients underwent hysterectomy for uterine myopathy in 54 patients with adenomyosis were selected as the study object and included in the uterine gland disease group. Select the same period in the hospital due to uterine fibroids or ovarian benign tumor hysterectomy in 50 patients with normal uterine muscle tissue into the control group (n=50). The expression of CD₁₁₇ in adenomyosis and normal myometrial tissue was studied by immunohistochemical staining. The relationship between CD₁₁₇ cells and uterus was studied by CD₁₁₇ and CD₃₄⁺ immunofluorescence staining. The procedures followed in this study are in line with the ethical standards set by the Human Body Test Committee of the First Affiliated Hospital of Harbin Medical University and are approved by the committee. The study obtained the informed consent of the subjects, and signed a clinical research informed consent.

CD₁₁₇ cells were found in the normal tissues of the adenomyosis group and the control group. CD₁₁₇ cells expressed higher in the lesion tissues of the adenomyosis group than that in the control group. The average CD₁₁₇ cells was (18±4), the control group was (8±3), the difference was statistically significant (t= 2.571, P<0.05). ② CD₃₄⁺ and CD₁₁₇ immunofluorescence staining showed that CD₁₁₇ positive cells were CD₁₁₇/CD₃₄^-cells under fluorescence microscope. There were more CD₃₄⁺ microvascular structures in the tissues of adenomyosis, CD₁₁₇ cells were distributed in the small blood vessels of adenomyosis, and CD₃₄⁺ expression was found in the endothelial cells of the vessel wall, and no positive CD₁₁₇⁺ expression was not found.

CD₁₁₇⁺ cells may be involved in the pathogenesis of adenomyosis.