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中华妇幼临床医学杂志(电子版)

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2018, Vol. 14 ›› Issue (05): 553 -557. doi: 10.3877/cma.j.issn.1673-5250.2018.05.010

Special Issue: ；

Original Article

Analysis of polymorphisms of -443C/T and -616G/T loci of osteopontin gene in children with Kawasaki disease

Xia Zhan1, Pengzhu Li2, Gui Xie2, Yujie Qian2, Lang Tian2, Jia Chen2, Zhuoying Li2, Jie Jiang2, Lihua Huang3, Zuocheng Yang2,†()   

  1. 1. Department of Pediatrics,Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China; Department of Pediatrics, First People′s Hospital of Changde City, Changde 415000, Hunan Province, China
    2. Department of Pediatrics,Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China
    3. Department of Center for Medical Experiments, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China
  • Received:2018-01-23 Revised:2018-09-05 Published:2018-10-01
  • Corresponding author: Zuocheng Yang
  • About author:
    Corresponding author: Yang Zuocheng, Email:
  • Supported by:
    Science and Technology Project of Science and Technology Bureall in Changsha, Hunan Province(kq1602042); Fund of " New Xiangya Talent Project" in Third Xiangya Hospital of Central South University(JY20150312)
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Objective

To explore the characteristics of polymorphisms of -443C/T and -616G/T loci in osteopontin (OPN) gene of children with Kawasaki disease, and association between coronary artery lesion (CAL) and these two loci.

Methods

A total of 104 children with Kawasaki disease who were admitted to Third Xiangya Hospital of Central South University from December 2006 to December 2014, were selected as research subjects (Kawasaki disease group). Using random number table method, another 100 health children who took physical examination in same hospital during the same period, were included into control group. Polymorphisms of -443C/T and -616G/T loci of OPN gene were detected by gene Sanger method in 204 subjects. Genotype frequency and allele frequency (AF) differences of two loci of OPN gene were compared between two groups of subjects by chi-square test, and the Pearson contingency coefficient (r) and independent chi-square test were used to analyze the correlation between CAL and polymorphisms of two loci of OPN gene in Kawasaki disease children. This study protocol was in line with the Institutional Review Board of Third Xiangya Hospital of Central South University (approval No. 2016-S155), and informed contents were obtained from the guardians of all subjects before they accepted the genetic testing.

Results

①The genotypes of -443C/T locus of OPN gene were CC, CT, TT, respectively, and the genotypes of -616G/T locus of OPN gene were GG, GT, TT, respectively. ②There were no significant differences between two groups of subjects in genotype frequency and AF of -443C/Tand -616G/T loci of OPN gene (P>0.05). ③CAL was not correlated with genotype frequency and AF of OPN gene -443C/T locus in children with Kawasaki disease (r=0.133, χ2=1.875, P=0.392; r=0.132, χ2=1.834, P=0.176); also was not correlated with genotype frequency and AF of OPN gene -616G/T locus in children with Kawasaki disease (r=0.130, χ2=1.775, P=0.412; r=0.059, χ2=0.365, P=0.546).

Conclusions

The polymorphisms of -443C/T and -616G/T loci of OPN gene were not yet found associated with occurrence of Kawasaki disease and concurrent CAL.

Key words: Mucocutaneous lymph node syndrome, Osteopontin, Polymorphism, genetic, Coronary artery lesion, Child
图2 骨桥蛋白基因－616G/T位点基因测序图(图2A：GG基因型；图2B：GT　基因型；图2C：TT　基因型)
表1 2组受试儿骨桥蛋白基因－443C/T、－616G/T位点的基因型频率及等位基因频率比较[例数(%)]
组别 例数 OPN基因－443C/T位点基因型 OPN基因－443C/T位点AF
CT TT CC T C
川崎病组 104 47(45.2) 46(44.2) 11(10.6) 139(66.8) 69(33.2)
对照组 100 56(56.0) 31(31.0) 13(13.0) 118(59.0) 82(41.0)
χ2值 ? ? 3.798 ? 2.679
P值 ? ? 0.150 ? 0.102
组别 例数 OPN基因－616G/T位点基因型 OPN基因－616G/T位点AF
GT TT GG T G
川崎病组 104 48(46.2) 33(31.7) 23(22.1) 114(54.8) 94(45.2)
对照组 100 49(49.0) 39(39.0) 12(12.0) 127(64.0) 73(36.0)
χ2值 ? ? 3.891 ? 3.186
P值 ? ? 0.143 ? 0.074
表2 川崎病冠状动脉病变与骨桥蛋白基因－443C/T、－616G/T位点多态性的关联性分析[例数(%)]
CAL 例数 OPN基因－443C/T位点基因型 OPN基因－443C/T位点AF
CT TT CC T C
有 43 18(41.8) 22(51.2) 3(7.0) 62(72.1) 24(27.9)
无 61 29(47.6) 24(39.3) 8(13.1) 77(63.1) 45(36.9)
χ2值 ? ? 1.875 ? 1.834
P值 ? ? 0.392 ? 0.176
CAL 例数 OPN基因－616G/T位点基因型 OPN基因－616G/T位点AF
GT TT GG T G
有 43 23(53.5) 11(25.6) 9(20.9) 45(52.3) 41(47.7)
无 61 25(41.0) 22(36.1) 14(22.9) 69(56.6) 53(43.4)
χ2值 ? ? 1.775 ? 0.365
P值 ? ? 0.412 ? 0.546
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