Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2017, Vol. 13 ›› Issue (05): 569 -573. doi: 10.3877/cma.j.issn.1673-5250.2017.05.012

Special Issue:

Original Article

Outcomes of different times of vitrification-thawed embryo transfer in freeze-all cycles paitents

Fang Lyu1, Luhua Yu1, Liping Wang2, Xueli Li1, Lin Xu1, Fang Chen1, Chunhui Zhu1, Kaifeng Liu1, Chenwang Zhang1, Li Wang3, Xiaomei Zhang1,()   

  1. 1. Reproductive Medicine Center
    2. Department of Biobank, Department of Obstetrics and Gynecology, Northern Jiangsu People′s Hospital, Yangzhou University, Yangzhou 225001, Jiangsu Province, China
    3. Department of Anesthesiology & Perioperative Medicine, MD Anderson Cancer Center, University of Texas, Houston 78712, USA
  • Received:2017-04-08 Revised:2017-08-01 Published:2017-10-01
  • Corresponding author: Xiaomei Zhang
  • About author:
    Corresponding author: Zhang Xiaomei, Email:
Objective

To explore the clinical outcomes of different times of frozen embryo transfer (FET) cycles in the freeze-all cycles.

Methods

A total of 807 patients who underwent all embryos cryopreservation between January 2012 and September 2016 were analyzed. According to the different times of FET cycles in the same oocyte retrieval, they were divided into 4 groups: patients underwent FET once as group A (n=543), twice FET as group B (n=181), three times FET as group C (n=63), and four times FET as group D (n=20). The clinical medical records of two groups were retrospectively analyzed. The general clinical data, transfer cycles and the rates of cumulative pregnancy and clinical pregnancy among 4 groups were analyzed.

Results

①There was no statistically difference with four groups in general characteristics which includes age, duration of infertility, infertility type, body mass index (BMI), basic follicle stimulating hormone (FSH) levels, basic luteinizing hormone(LH)levels, basic estradiol levels, basic testosterone levels, optimum number of oocytes, number of metaphase Ⅱ(MⅡ) oocytes, normal fertilization rate, number of available embryos(P>0.05). ②There was no statistically difference with four groups′clinical data including endometrial thickness, embryo number transferred, prime embryo rate and artificial cycle rate for endometrial preparation program (P>0.05). ③The cumulative pregnancy rates per collection cycle of 4 groups were 64.5%, 60.0%, 58.7% and 60.0%, respectively. And there was no significant difference (χ2=1.67, P=0.64). The clinical pregnancy rates were 64.5%, 36.5%, 25.1% and 18.8%, respectively. And there was significant difference(χ2=143.02, P<0.01).

Conclusions

The increasing of the number of FET transplantation in the same oocyte retrieval were not increased the total pregnancy rate of freeze-all cycles, but decreased the clinical pregnancy rate.

表1 4组患者一般临床资料比较
组别 例数 年龄(岁,±s) 不孕年限(年,±s) BMI(kg/m2±s) 不孕类型[例数(%)]
原发不孕 继发不孕
A组 543 30.6±0.2 3.9±0.1 22.4±0.1 332(61.1) 211(38.9)
B组 181 29.9±0.2 3.9±0.1 22.5±0.6 112(62.2) 69(37.8)
C组 63 31.0±0.6 3.9±0.6 22.3±0.4 31(49.2) 32(50.8)
D组 20 31.7±1.0 4.1±0.8 23.4±0.6 11(55.0) 9(45.0)
检验值 ? F=1.41 F=0.06 F=1.72 χ2=3.82
P ? 0.24 0.98 0.16 0.28
组别 例数 不同因素全胚冷冻率[例数(%)] 基础FSH水平(IU/L,±s) 基础LH水平(IU/L,±s) 基础雌二醇水平(pmol/L,±s)
预防OHSS 内膜因素 其他因素
A组 543 178(32.8) 19(3.5) 346(63.7) 6.4±0.1 5.1±0.1 132.2±3.9
B组 181 58(32.0) 7(3.9) 116(64.1) 6.3±0.1 4.8±0.1 127.4±3.8
C组 63 15(23.8) 2(3.2) 46(73.0) 6.0±0.2 8.7±3.1 125.8±5.3
D组 20 8(40.0) 0(0.0) 12(60.0) 5.4±0.2 6.7±0.8 142.5±7.4
检验值 ? χ2=2.67 χ2=0.83 χ2=2.34 F=0.89 F=5.37 F=0.47
P ? 0.45 0.84 0.51 0.45 0.11 0.71
组别 例数 基础睾酮水平(nmol/L,±s) 基础AFC(个,±s) 获卵数(个,±s) MⅡ卵母细胞数(个,±s) 正常受精率(%,±s) 同一取卵周期可移植胚胎率(%,±s)
A组 543 1.1±0.2 13.1±0.2 12.0±0.4 9.5±0.6 66.5±10.4 68.4±7.9
B组 181 0.8±0.0 13.3±0.2 11.0±0.3 9.1±0.1 68.8±11.4 71.9±9.1
C组 63 0.8±0.1 13.7±0.5 11.3±0.5 9.1±0.3 68.7±9.4 69.4±10.8
D组 20 1.1±0.1 13.8±1.0 11.4±0.5 9.7±0.3 65.8±8.6 70.4±8.0
检验值 ? F=0.41 F=0.46 F=1.16 F=19.16 F=4.09 F=2.37
P ? 0.75 0.71 0.26 0.11 0.22 0.36
表2 4组患者冻融胚胎移植情况比较
表3 4组患者累计临床妊娠率和临床妊娠率比较[例数(%)]
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