Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2015, Vol. 11 ›› Issue (03): 383 -387. doi: 10.3877/cma.j.issn.1673-5250.2015.03.021

Special Issue:

Original Article

Study on the relationship between genetic polymorphisms of cadherin-related family member 3, interleukin-33 and DNA repair protein 50 and childhood bronchial asthmatic susceptibility

Yongfeng Yu1, Tang Li1()   

  1. 1. Department of Pediatrics, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China
  • Received:2015-03-12 Revised:2015-04-25 Published:2015-06-01
  • Corresponding author: Tang Li
  • About author:
    Corresponding author: Li Tang, Email:
Objective

To evaluate the potential relationship between polymorphisms of cadherin-related family member(CDHR)3, interleukin(IL)-33 and DNA repair protein(RAD)50 and bronchial asthma.

Methods

From December 2012 to June 2014, a total of 100 children with bronchial asthma who were diagnosed and treated at the Affiliated Hospital of Qingdao University were included in the study as bronchial asthma group(n=100). Meanwhile, 100 healthy volunteers were recruited for this study as control group(n=100). The CDHR3, IL-33, RAD50 gene were analyzed by single nucleotide polymorphisms technique.The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Affiliated Hospital of Qingdao University.Informed consents were obtained from each participants' parents.

Results

The alleles of CDHR3, IL-33, RAD50 gene were in Hardy-Weinburg balance.There were no significant differences in rs6967330 of CDHR3 and rs928413 of IL-33 gene polymorphisms between two groups(χ2=3.442, 3.071, 2.895, 2.829; P=0.179, 0.088, 0.235, 0.124). Significant differences were found in rs6871536 of RAD50 gene polymorphisms between two groups(χ2=6.635, 9.200; P=0.036, 0.003).

Conclusions

The rs6967330 and rs 928413 polymorphisms were not correlated with asthmatic susceptibility, rs6871536 polymorphisms may be the susceptible genotype of bronchial asthma in children.

表1 CDHR3、RAD50、IL-33基因SNP位点引物序列
Table 1 SNP primer sequence of CDHR3,RAD50,IL-33 gene
表2 Sequenom MassArray系统基因分型扩增PCR反应组分
Table 2 Components and reagents of amplification PCR in Sequenom MassArray system
表3 两组CDHR3基因rs6967330位点基因型与等位基因频率分布比较[例数(%)]
Table 3 Comparison of rs6967330 of CDHR3 gene polymorphisms between two groups[case(%)]
表4 两组IL-33基因rs928413位点基因型与等位基因频率分布比较[例数(%)]
Table 4 Comparison of rs928413 of IL-33 gene polymorphisms between two groups[case(%)]
表5 两组RAD50基因rs6871536位点基因型与等位基因频率分布比较[例数(%)]
Table 5 Comparison of rs6871536 of RAD50 gene polymorphisms between two groups[case(%)]
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