The Role of Foxp3, CD4+CD25+ Regulatory T Cells on the Pathogenesis of Infectious Mononucleosis in Children

doi:10.3877/cma.j.issn.1673-5250.2013.02.008

Objective

To investigate the role of CD₄⁺CD₂₅⁺ regulatory T cells(Treg) and transcription factor Foxp3 on the pathogenesis of infectious mononucleosis(IM) in children.

Methods

From April 2010 to January 2011, 70 blood samples from 35 IM children(2 samples each person) were included into this study, and divided into IM acute stage group(n=35) and IM recovery stage group(n=35) according to the different disease periods.Meanwhile, 35 blood samples which taken from health children (1 sample each person) were chosen as control group(n=35). The expression of T lymphocyte subsets CD₃⁺, CD₃⁺CD₄⁺, CD₃⁺CD₈⁺, CD₄⁺CD₂₅⁺ Treg and Foxp3 mRNA were compared among three groups. There was no significant difference in age and sex distribution between IM patients and health chirdren (P>0.05). The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Sichuan Province People's Hospital.Informed consent was obtained from each parents.

Results

There had significant differences among three groups in relative expression levels of CD₃⁺, CD₃⁺CD₄⁺, CD₃⁺CD₈⁺, CD₄⁺CD₂₅⁺ Treg (P<0.01). The relative levels of Foxp3 mRNA in IM acute stage group(2.82±0.90) were obviously lower than those of IM recovery stage group(4.11±1.37) and control group (4.65±1.23) (P<0.01). There was no significant difference between IM recovery stage group and control group(P>0.05). A significant positive correlation was found between CD₄⁺CD₂₅⁺ Treg and expression of Foxp3 mRNA in IM acute stage group(r=0.823, P<0.05).

Conclusions

There is functional disorder of T lymphocyte subsets at acute stage of IM in children. The relative expression levels of CD₄⁺CD₂₅⁺ Treg were reduced, and their special transcription factor Foxp3 mRNA were decreased, which led to the insufficient immunosuppressive effects that may be one of the important reasons of the immune imbalance.

Key words: Foxp3, CD₄⁺CD₂₅⁺ regulatory T cell, infectious mononucleosis, child

表1 3组CD₃⁺，CD₃⁺ CD₄⁺，CD₃⁺CD₈⁺，CD₄⁺/CD₈⁺的相对表达水平比较(±s)

Table 1 Comparison of the relative expression levels of CD₃⁺，CD₃⁺CD₄⁺，CD₃⁺CD₈⁺，CD₄⁺/CD₈⁺ among three groups(±s)

组别	n	CD₃⁺	CD₃⁺ CD₄⁺	CD₃⁺CD₈⁺	CD₄⁺/CD₈⁺
IM急性期组	35	80.66±4.88	16.53±5.55	62.71±8.76	0.26±0.12
IM恢复期组	35	74.25±7.33	29.89±6.03	42.25±7.33	0.71± 0.19
对照组	35	63.04±5.51	37.98±4.20	27.71±6.52	1.37±0.45
F		49.98	107.23	139.82	120.53
P		<0.01	<0.01	<0.01	<0.01

表1 3组CD₃⁺，CD₃⁺ CD₄⁺，CD₃⁺CD₈⁺，CD₄⁺/CD₈⁺的相对表达水平比较(±s)

Table 1 Comparison of the relative expression levels of CD₃⁺，CD₃⁺CD₄⁺，CD₃⁺CD₈⁺，CD₄⁺/CD₈⁺ among three groups(±s)

组别	n	CD₃⁺	CD₃⁺ CD₄⁺	CD₃⁺CD₈⁺	CD₄⁺/CD₈⁺
IM急性期组	35	80.66±4.88	16.53±5.55	62.71±8.76	0.26±0.12
IM恢复期组	35	74.25±7.33	29.89±6.03	42.25±7.33	0.71± 0.19
对照组	35	63.04±5.51	37.98±4.20	27.71±6.52	1.37±0.45
F		49.98	107.23	139.82	120.53
P		<0.01	<0.01	<0.01	<0.01

表2 3组Foxp3 mRNA，CD₄⁺CD₂₅⁺ Treg相对表达水平比较(±s)

Table 2 Comparison of the relative expression levels of Foxp3 mRNA, CD₄⁺CD₂₅⁺ Treg among three groups(±s)

组别	n	Foxp3 mRNA	CD₄⁺CD₂₅⁺ Treg
IM急性期组	35	2.82±0.90	2.38±1.32
IM恢复期组	35	4.11±1.37	6.81±1.84
对照组	35	4.65±1.23	7.85±1.97
F		23.92	30.61
P		<0.01	<0.01

表2 3组Foxp3 mRNA，CD₄⁺CD₂₅⁺ Treg相对表达水平比较(±s)

Table 2 Comparison of the relative expression levels of Foxp3 mRNA, CD₄⁺CD₂₅⁺ Treg among three groups(±s)

组别	n	Foxp3 mRNA	CD₄⁺CD₂₅⁺ Treg
IM急性期组	35	2.82±0.90	2.38±1.32
IM恢复期组	35	4.11±1.37	6.81±1.84
对照组	35	4.65±1.23	7.85±1.97
F		23.92	30.61
P		<0.01	<0.01

图1 CD₄⁺CD₂₅⁺Treg与Foxp3 mRNA的相关性分析图

Figure 1 The scatter of CD₄⁺CD₂₅⁺Treg and Foxp3 mRNA

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The Role of Foxp3, CD₄⁺CD₂₅⁺ Regulatory T Cells on the Pathogenesis of Infectious Mononucleosis in Children

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The Role of Foxp3, CD4+CD25+ Regulatory T Cells on the Pathogenesis of Infectious Mononucleosis in Children

The Role of Foxp3, CD₄⁺CD₂₅⁺ Regulatory T Cells on the Pathogenesis of Infectious Mononucleosis in Children