To explore the influence of oxygen content changes on trophoblast cells viability and Mg²⁺ protective effect on trophoblast cells.

From April 2005 to April 2006, sterile villi were obtained from 20 healthy women with 8 to 10 pregnant weeks. All selected subjects were excluded if there were some factors that would be considered to effect results. The trophoblastic cells of primary culture from sterile villi were divided into the following four groups to culture: Control group, hypoxia group, hypoxia-reoxygenation group, and hypoxia- reoxygenation with Mg²⁺ group (0.6 mmol/L) . The MTT colorimetric assay was used to analyze the cell viability. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of the First Affiliated Hospital of GuangXi Medical University. Informed consent was obtained from all participates.

①The viability of trophoblastic cells in hypoxia group had significant differences from 24-hour to 48-hour compared with control group (P<0.05), but without statistical significance at the time of 72 hours (P>0.05). ②There was statistically significant difference between hypoxia-reoxygenation group and hypoxia group and control group (P<0.05). ③The difference of viability of trophoblastic cells was significance between hypoxia-reoxygenation group and hypoxia-reoxygenation group with Mg²⁺ (P<0.05).

In early hypoxia, the viability of trophoblastic cells is enhance and the Mg²⁺ has protective effect for the cells suffered the hypoxia-reoxygenation process.