Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2013, Vol. 09 ›› Issue (05): 594 -598. doi: 10.3877/cma.j.issn.1673-5250.2013.05.005

Special Issue:

Original Article

Expression and Significance of Genetic Imprinted Gene of Paternally Expressed Gene 10 in Placenta of Preeclampsia

Xiao-yan LIANG1, Xiong CHEN1(), Xia CHEN1   

  1. 1. Department of Obstetrics and Gynecology, Baoshan Branch of Shanghai First People's Hospital, Shanghai 200940, China
  • Received:2013-05-11 Revised:2013-08-24 Published:2013-10-01
  • Corresponding author: Xiong CHEN
  • About author:
    (Corresponding author: CHEN Xiong, Email: )
Objective

To detect the expression and significance of genetic imprinted gene of paternally expressed gene 10 (PEG10) in placenta of preeclampsia (PE) and normal pregnancy.

Methods

The expression and distribution of PEG10 in placenta tissues of 22 patients with PE (study group) and 22 normal pregnant women (control group) were examined by Real-time FQ-PCR and immunohistochemistry between August 2010 to June 2012 at Baoshan Branch of Shanghai First People's Hospital. There had no significant differences between two groups on age, gestational age, gravidity, and parity (P>0.05). The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Baoshan Branch of Shanghai First People's Hospital. Informed consent was obtained from all participates.

Results

The expression of placenta PEG10 mRNA and protein were found both in two groups with significant differences (0.5832±0.0450 vs. 0.1943±0.035, 0.1258±0.0860 vs. 0.0572±0.0050; t=6.8266, P<0.001; t=2.4296, P<0.01).

Conclusions

Up-regulation of PEG10 may be one of the early manifestations of molecular markers of PE.

表1 2组胎盘组织中PEG10 mRNA及蛋白的表达水平(±s)
Table 1 PEG10 mRNA expression levels of placenta tissues between two groups (±s)
图1 正常妊娠与子痫前期患者胎盘组织基因PEG10扩增动力曲线
Figure 1 Normal pregnancy and preeclampsia patients with placenta tissue PEG10 amplification power curve
图2 免疫组化分析PEG10在正常与子痫前期胎盘组织的定位与表达(×100) A:子痫前期胎盘组织免疫组化产物;B:正常妊娠胎盘组织免疫组化产物
Figure 2 Immunohistochemical analysis of PEG10 protein expression in placenta tissue from preeclampsia patients and NP (×100)(A:Preeclampsia patients sample;B:2 NP sample)
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