Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2010, Vol. 06 ›› Issue (02): 96 -99. doi: 10.3877/cma.j.issn.1673-5250.2010.02.006

Original Article

Correlation Among Plasma Homocysteine, Oxidized Low-Density Lipoprotein, Malondialdehyde, and Body Mass Index in Obese Children

Yu WANG, Wen-xian CAI, Feng-hai NIU, Xue-yun REN, Ji-ping SHAN   

  1. Department of Pediatrics, Affiliated Hospital of Jining Medical College, Jining 272129, Shandong Province, China
  • Published:2010-04-01
Objective

To investigate the correlation of plasma homocysteine(Hcy), oxidized low-density lipoprotein(OX-LDL), malondialdehyde (MDA), and body mass index(BMI) in obese children and their clinical significances.

Methods

From January 2007 to December 2007, 30 children, 17 boys and 13 girls, aged 7-12, were diagnosed as secondary obesity (group A, n=30) according to the diagnosis standard of children's obesity, that is, the height and weight exceed 20% of the ordinary children of the same age, and they have illness history of having much food and little exercise which corresponds with the adiposity. They have well-proportioned body and no heart or lung diseases, no consequent fatness caused by incretion, metabolism, or central nervous system diseases. Meanwhile, another 30 healthy children, 16 boys and 14 girls, aged 7-12, were chosen to control group (group B, n=30). Informed consent was obtained from all participants. The whole procedure of this study was conformed to the ethic standard set by the human body experiment committee of the hospital and the study has been approved by this committee. The children involved in this experiment are clear about the whole process and their guardians have signed an agreement. There had no statistic significance in age and gender between two groups (P>0.05). Levels of oxidized low-density lipoprotein, homocysteine were measured by enzyme linked immunosorbent assay(ELISA). The concentration of plasma malondialdehyde was measured by thiobarbituric acid reactive substance(TBARS) method. All children were avoided to eat high-protein food for 3 days and fasted for 12 hours before the blood samples were taken.

Results

No significant homocysteine level differences were found in boys and girls between two groups(P>0.05). Body mass index, homocysteine, malondialdehyde, oxidized low-density lipoprotein in group A were significantly higher than those of group B. Significant correlation between homocysteine level and body mass index (r=0.44, P<0.05), homocysteine level and malondialdehyde (r=0.47, P<0.01) were found in group A. There was not any relations between homocysteine levels and oxidized low-density lipoprotein (r=0.30, P>0.05).

Conclusion

There was an increased oxidative stress in obese children, and the increase of homocysteine level was closely associated with lipid peroxidation. Abnormal lipid metabolism exists in obese children.

1 Bovet P, Chiolero A, Madeleine G, et al. Marked increase in the prevalence of obesity in children of the Seychelles, a rapidly developing country, between 1998 and 2004[J]. Int J Pediatr Obes, 2006, 1(2): 120-128.
2 Lob CT. Overweight and obesity in childhood--a special challenge for public health[J]. Int J Hyg Environ Health, 2007, 210(5): 585-589.
3 Salvadori M, Sontrop JM, Garg AX, et al. Elevated blood pressure in relation to overweight and obesity among children in a rural Canadian community[J]. Pediatrics, 2008, 122(4): 821-827.
4 Gelbrich G, Blüher S, Reich A, et al. Prevalence of obesity and elevated blood pressure as well as onset of puberty in German school children attending different educational tracks[J]. Horm Res, 2008, 70(6): 340-348.
5 Hu YM, Jiang ZF, ed. Zhu Futang textbook of pediatrics.7 th[M]. Beijing: People's Medical Publishing House, 2002, 552-554. [胡亚美,江载芳,主编.诸福棠实用儿科学. 7版[M].北京:人民卫生出版社,2002, 552-554.]
6 Chu NF, Pan WH. Prevalence of obesity and its comorbidities among schoolchildren in Taiwan[J]. Asia Pac J Clin Nutr, 2007, 2: 601-607.
7 Spiotta RT, Luma GB. Evaluating obesity and cardiovascular risk factors in children and adolescents[J]. Am Fam Physician, 2008, 78(9): 1052-1058.
8 Raj M, Sundaram KR, Paul M, et al. Obesity in Indian children: Time trends and relationship with hypertension[J]. Natl Med J India, 2007, 20(6): 288-293.
9 Khositseth A, Suthutvoravut U, Chongviriyaphan N, et al. Cardiac dimensions and function in children with obesity[J]. Indian Heart J, 2006, 58(6): 422-425.
10 Reeves GM, Postolache TT, Snitker S. Childhood Obesity and Depression: Connection between these growing problems in growing children[J]. Int J Child Health Hum Dev, 2008, 1(2): 103-114.
11 Angelopoulos PD, Milionis HJ, Moschonis G, et al.Relations between obesity and hypertension: Preliminary data from a cross-sectional study in primary school children. The children study[J]. Eur J Clin Nutr, 2006, 60(10): 1226-1234.
12 Huus K, Ludvigsson JF, Enskar K, et al. Risk factors in childhood obesity-findings from the All Babies In Southeast Sweden (ABIS) cohort[J]. Acta Paediatr, 2007, 96(9): 1321-1325.
13 Jolliffe CJ, Janssen I. Vascular risks and management of obesity in children and adolescents[J].Vasc Health Risk Manag, 2006, 2(2): 171-187.
14 Kortelainen ML. Adiposity, cardiac size and precursors of coronary atherosclerosis in 5 to 15-year-old children: A retrospective study of 210 violent deaths[J]. Int J Obes Relat Metab Disord, 1997, 21(8): 691-697.
15 Wotherspoon F, Laight DW, Turner C, et al. The effect of oral folic acid upon plasma homocysteine, endothelial function and oxidative stress in patients with type 1 diabetes and microalbuminuria[J]. Int J Clin Pract, 2008, 62(4): 569-574.
16 Ni M, Zhang XH, Jiang SL, Zhang Y. Homocysteinemia as an independent risk factor in the Chinese population at a high risk of coronary artery disease[J]. Am J Cardiol, 2007, 100(3): 455-458.
17 Jin L, Caldwell RB, Li-Masters T, et al. Homocysteine induces endothelial dysfunction via inhibition of arginine transport[J]. J Physiol Pharmacol, 2007, 58(2): 191-206.
18 Myles PS, Chan MT, Kaye DM, et al. Effect of nitrous oxide anesthesia on plasma homocysteine and endothelial function[J]. Anesthesiology, 2008, 109(4): 657-663.
19 Linnebank M, Moskau S, Farmand S, et al. Homocysteine and carotid intima-media thickness in a german population: Lack of clinical relevance[J]. Stroke, 2006, 37(11): 2840-2842.
20 Lauricella AM, Quintana I, Castanon M, et al. Influence of homocysteine on fibrin network lysis[J]. Blood Coagul Fibrinolysis, 2006, 17(3): 181-186.
21 Undas A, Brozek J, Jankowski M, et al. Plasma homocysteine affects fibrin clot permeability and resistance to lysis in human subjects[J]. Arterioscler Thromb Vasc Biol, 2006, 26(6): 1397-1404.
22 Sauls DL, Lockhart E, Warren ME, et al. Modification of fibrinogen by homocysteine thiolactone increases resistance to fibrinolysis: A potential mechanism of the thrombotic tendency in hyperhomocysteinemia[J]. Biochemistry, 2006, 45(8): 2480-2487.
23 Youssef MY, Mojiminiyi OA, Abdella NA. Plasma concentrations of C-reactive protein and total homocysteine in relation to the severity and risk factors for cerebrovascular disease[J]. Transl Res, 2007, 150(3): 158-163.
24 Itabe H, Ueda M. Measurement of plasma oxidized low-density lipoprotein and its clinical implications[J]. J Atheroscler Thromb, 2007, 14(1): 1-11.
25 Matsuura E, Hughes GR, Khamashta MA, et al. Oxidation of LDL and its clinical implication[J]. Autoimmun Rev, 2008, 7(7): 558-566.
26 Paul A, Chang BH, Li L, et al. Deficiency of adipose differentiation-related protein impairs foam cell formation and protects against atherosclerosis[J]. Circ Res, 2008, 102(12): 1492-1501.
27 Gleissner CA, Sanders JM, Nadler J, et al. Upregulation of aldose reductase during foam cell formation as possible link among diabetes, hyperlipidemia, and atherosclerosis[J]. Arterioscler Thromb Vasc Biol, 2008, 28(6): 1137-1143.
28 Ma FX, Zhou B, Chen Z, et al. Oxidized low density lipoprotein impairs endothelial progenitor cells by regulation of endothelial nitric oxide synthase[J]. J Lipid Res, 2006, 47(6): 1227-1237.
29 Devrim E, Erten S, Erguder IB, et al. Malondialdehyde and nitric oxide levels in erythrocytes from patients with systemic sclerosis[J]. Med Princ Pract, 2008, 17(4): 349-350.
30 Bamonti F, Novembrino C, Ippolito S, et al. Increased free malondialdehyde concentrations in smokers normalise with a mixed fruit and vegetable juice concentrate: A pilot study[J]. Clin Chem Lab Med, 2006, 44(4): 391-395.
[1] Jinmei He, Lixue Yin, Jing Tan, Wenjun Zhang, Rui Wang, Mei Ren, Mingjiao Liao. Evaluation of potential left ventricular systolic dysfunction in patients with type 2 diabetes mellitus using ultrasonic myocardial work technology[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(10): 1029-1035.
[2] Xuan Zhang, Yutong Ma, Yuqian Miao, Yun Zhang, Shiwen Wu, Xiaochu Dang, Yingying Chen, Zhaoming Zhong, Xuejuan Wang, Miao Hu, Yanfeng Sun, Xiuzhu Ma, Faqin Lyu, Haiyan Kou. Ultrasound assessment of diaphragm function in pediatric patients with Duchenne muscular dystrophy[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(10): 1068-1073.
[3] Baofu Zhang, Jin Yu, Jingjing Ye, Jiangen Yu, Xiaohui Ma, Xiwang Liu. Echocardioimagedata diagnosis of anomalous pulmonary venous connection caused by congenital malposition of the septum primum[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(10): 1074-1080.
[4] Dan Han, Ting Wang, Huan Xiao, Lirong Zhu, Jingyu Chen, Yi Tang. Diagnostic value of contrast enhanced ultrasound versus contrast enhanced computed tomography in benign and malignant liver lesions in children[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(09): 939-944.
[5] Tingting Liu, Yanbing Lin, Shan Wang, Murong Chen, Zijian Tang, Dongling Dai, Bei Xia. Evaluation of metabolic dysfunction-associated fatty liver disease in children by ultrasound-guided attenuation parameter[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(08): 787-794.
[6] Yuhan Zhou, Huan Xiao, Chunjiang Yang, Juan Zhou, Lirong Zhu, Juan Xu, Fangting Mou. Diagnostic value of ultrasound in children with temporary hip synovitis[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(08): 795-800.
[7] Jiu Wang, Jun Chen, Xia Zhu, Yangjin Mima, Sheng Zhao, Xinlin Chen, Jianhua Li, Shuang Wang. Effect of implementing fetal systemic ultrasound screening in Material and Child Health Hospital of Shannan[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(07): 728-733.
[8] Jie Mi, Chen Chen, Jialing Li, Haina Pei, Hengbo Zhang, Fei Li, Dongjie Li. Analysis of the characteristics of children's head and face trauma[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(06): 511-515.
[9] Kailun Ji, Shaolong Hao, Haitao Sun, Wei Han. New progress of gallstone formation mechanism after weight loss[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(01): 100-103.
[10] Damin Chen, Xiaogang Cao, Nengqi Cao. Effect of obesity on surgical treatment of gastric cancer patients[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2023, 17(06): 651-653.
[11] Tao Ma, Chunwei Ye, Tao Liu, Wenxi Peng, Zhipeng Li. Comparison of laparoscopic and open disconnected pyeloplasty in the treatment of ureteropelvic junction obstruction in children[J]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(06): 605-610.
[12] Xiangyu Zhu, Jianmei Wang, Hui Zhang, Hongying Ye. Correlation analysis between left ventricular function parameters and liver cirrhosis using non-invasive left ventricular pressure-strain cycle[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2023, 13(06): 494-498.
[13] Shaohong Zhuo, Xiuling Lin, Cuimei Zhou, Weilian Xiong, Xingzao Ma. Application value of CD64 index combined with serum SAA/CRP and PCT in the diagnosis of children with infectious gastrointestinal diseases[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2023, 13(06): 505-509.
[14] Zehui Huang, Jiexian Liang, Wei Zeng. Application of dexmedetomidine combined with esketamine in painless gastroscopy in children[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2023, 13(06): 510-513.
[15] Jing Li, Lingling Zhang, Wei Xing. Value of concept of interest induction before anesthesia induction in pediatric surgery and its effect on family satisfaction[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(07): 812-817.
Viewed
Full text


Abstract