The expression and significance of hypoxia inducible factor 1α following exposure to hypoxia and/orischemia in newborn rats

doi:10.3877/cma.j.issn.1673-5250.2007.03.102

Objective

To investigate the expression, distribution and significance of hypoxia inducible factor la (HIF-1α) following exposure to hypoxia and (or) ischemia in newborn rats.

Methods

Postnatal 10 days SD rats underwent hypoxia, ischemia, hypoxia-ischemia, or sham surgery. HIF-1α expression and distribution were detected with immunohistochemistry.

Results

HIF-1α expression significantly increased at 4 h following hypoxia, ischemia, or hypoxia-ischemia compared to sham controls. Furthermore, HIF-1α expression peaked at 8 h following hypoxia and hypoxia-ischemia, but at 24 h following ischemia alone. HIF-1α was distributed in important functional areas of the brains, such as the cortex and hippocampus.

Conclusion

Timing and pattern of HIF-1α up regulation varied by type of brain injury. The expression and distribution of HIF-1α following brain injury suggests that HIF-1α may be related to cellular repair.

Key words: hypoxia inducible factor 1α(HIF-1α), newborn, brain damage

图1　免疫组化示不同组HIF-1 α蛋白的表达，A假手术组有少量表达；B单纯缺氧组表达明显增多；C单纯缺血组表达较B少；D缺氧缺血组表达较C少。B组、C组、D组三组HIF-1 α蛋白的表达均明显高于A组

图2　各组在不同时间点HIF-1 α的表达，假手术组各时间点表达相当；单纯缺氧组8 h表达最高；单纯缺血组24 h表达最高；缺氧缺血组8 h表达最高。表达高峰时间比较，单纯缺氧组最高，其次是单纯缺血组，再次是缺氧缺血组

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