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中华妇幼临床医学杂志(电子版) ›› 2013, Vol. 09 ›› Issue (06) : 764 -768. doi: 10.3877/cma.j.issn.1673-5250.2013.06.011

所属专题: 文献

论著

未见原核晚期卵裂胚胎染色体的微阵列-比较基因组杂交分析
张守信1,*,*(), 刘晓妍2, 孙成铭1, 刘杰1, 郝翠芳2   
  1. 1. 264000 山东省烟台毓璜顶医院生物芯片实验室
    2. 264000 山东省烟台毓璜顶医院生殖医学中心
  • 收稿日期:2013-06-11 修回日期:2013-11-02 出版日期:2013-12-01
  • 通信作者: 张守信

Aneuploidy Analysis of Non-Pronuclei Embryos from in Vitro Fertilization With Microarray Comparative Genomic Hybridization

Shou-xin ZHANG1(), Xiao-yan LIU2, Cheng-ming SUN1, Jie LIU1, Cui-fang HAO2   

  1. 1. Biochip Lab, Yantai Yuhuangding Hospital, Yantai 264000, Shandong Province, China
  • Received:2013-06-11 Revised:2013-11-02 Published:2013-12-01
  • Corresponding author: Shou-xin ZHANG
  • About author:
    (Corresponding author : ZHANG Shou-xin, Email : )
引用本文:

张守信, 刘晓妍, 孙成铭, 刘杰, 郝翠芳. 未见原核晚期卵裂胚胎染色体的微阵列-比较基因组杂交分析[J]. 中华妇幼临床医学杂志(电子版), 2013, 09(06): 764-768.

Shou-xin ZHANG, Xiao-yan LIU, Cheng-ming SUN, Jie LIU, Cui-fang HAO. Aneuploidy Analysis of Non-Pronuclei Embryos from in Vitro Fertilization With Microarray Comparative Genomic Hybridization[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2013, 09(06): 764-768.

目的

观察体外受精-胚胎移植(IVF-ET)治疗周期中未见原核(PN)晚期卵裂胚胎单个卵裂球染色体分子核型。

方法

取IVF-ET治疗中4枚未见PN晚期卵裂胚胎进行体外培养,在受精第3天行胚胎活检,对单个卵裂球行微阵列-比较基因组杂交(microarray-CGH)染色体分析。

结果

4枚未见PN受精卵染色体均发生非整倍体变异。其中1枚胚胎性染色体为X染色体单体,3枚胚胎性染色体为XY正常二倍体。但4枚胚胎均存在常染色体变异。

结论

未见PN晚期卵裂胚胎染色体microarray CGH分析结果表明,其单个卵裂球全基因组呈非整倍性变异,未见PN晚期卵裂胚胎存在常染色体和性染色体变异风险。

Objective

To analyze the aneuploidy of single blastomeres from nonpronuclear embryos on cleavage-stage in in vitro fertilization and embryo transfer (IVF-ET) cycle by microarray comparative genomic hybridization (microarray-CGH).

Methods

The four non-pronuclei embryo were achieved in one IVF cycle, and microarray-CGH was applied to analyze the aneuploidy of single cells biopsied from the four cleavage-stage embryos on day 3 after insemination.

Results

The four blastomere had non euploid variation in 24 chromosomes. Out of them, one had X monomer in sex chromosome, and the other three had normal XY diploid in sex chromosome. The four embryos had autosomal mutations, such as gene deletion.

Conclusions

The aneuploidy analysis by microarray-CGH shows that the blastomeres from nonpronuclear embryos on cleavage-stage in IVF cycle are aneuploid,and nonpronuclear embryos have the risk of abnormal sex chromosome and autosome chromosomes.

图1 4枚未见原核胚胎单个卵裂球行microarray-CGH 24条染色体非整倍性分析结果
Figure 1 Ratio profiles resulted after the microarray-CGH analysis of single blastomer 24 chromosome aneuploidy screening biopsied from four 0 PN embryo
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