探讨氧含量变化对滋养细胞生存能力的影响及抗氧化剂镁离子(Mg^2＋)对滋养细胞的保护作用。

2005年4月至2006年4月，获取在本院接受治疗的20例妊娠8～10孕周健康妇女的无菌绒毛组织，通过滋养细胞原代培养后，再进行不同方式的培养。按照不同培养方式，将滋养细胞分为对照组、缺氧组、缺氧后复氧组及Mg^2＋缺氧后复氧组(Mg^2＋0.6 mmol/L)。以四甲基偶氮唑(methyl thiazolyl tetrazolium，MTT)盐比色法(MTT法)测定以上4组滋养细胞的生存能力水平(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准，得到该委员会批准)。

①按照不同培养方式培养(24～48)h时，MTT法测定对照组、缺氧组滋养细胞的生存能力水平，两组比较，差异有显著意义(P<0.05)，72 h时，差异无显著意义(P>0.05)；②缺氧后复氧组和对照组、缺氧组MTT法测定的滋养细胞的生存能力水平比较，差异有显著意义(P<0.05)；③Mg^2＋缺氧后复氧组MTT值高于缺氧后复氧组，差异有显著意义(P<0.05)。

缺氧初期可导致滋养细胞生存能力增强，缺氧后复氧环境下滋养细胞生存能力降低，Mg^2＋对缺氧后复氧细胞有保护作用。

To explore the influence of oxygen content changes on trophoblast cells viability and Mg²⁺ protective effect on trophoblast cells.

From April 2005 to April 2006, sterile villi were obtained from 20 healthy women with 8 to 10 pregnant weeks. All selected subjects were excluded if there were some factors that would be considered to effect results. The trophoblastic cells of primary culture from sterile villi were divided into the following four groups to culture: Control group, hypoxia group, hypoxia-reoxygenation group, and hypoxia- reoxygenation with Mg²⁺ group (0.6 mmol/L) . The MTT colorimetric assay was used to analyze the cell viability. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of the First Affiliated Hospital of GuangXi Medical University. Informed consent was obtained from all participates.

①The viability of trophoblastic cells in hypoxia group had significant differences from 24-hour to 48-hour compared with control group (P<0.05), but without statistical significance at the time of 72 hours (P>0.05). ②There was statistically significant difference between hypoxia-reoxygenation group and hypoxia group and control group (P<0.05). ③The difference of viability of trophoblastic cells was significance between hypoxia-reoxygenation group and hypoxia-reoxygenation group with Mg²⁺ (P<0.05).

In early hypoxia, the viability of trophoblastic cells is enhance and the Mg²⁺ has protective effect for the cells suffered the hypoxia-reoxygenation process.