Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2018, Vol. 14 ›› Issue (01): 77 -84. doi: 10.3877/cma.j.issn.1673-5250.2018.01.013

Special Issue:

Original Article

Study on long non-coding RNA gene expression profile of follicular fluid in polycystic ovary syndrome patients

Liping Wang1, Fang Lyu2, Xueli Li2, Yu Pan2, Xiaoqin Lyu2, Xiao He2, Li Wang3, Xiaomei Zhang2,()   

  1. 1. Department of Biobank, Department of Obstetrics and Gynecology, Clinical Medical School of Yangzhou University·Northern Jiangsu People′s Hospital, Yangzhou University, Yangzhou 225001, Jiangsu Province, China
    2. Reproductive Medicine Center, Department of Obstetrics and Gynecology, Clinical Medical School of Yangzhou University·Northern Jiangsu People′s Hospital, Yangzhou University, Yangzhou 225001, Jiangsu Province, China
    3. John Hopkins School of Medicine, Baltimore 21218, Maryland, United States of America
  • Received:2017-10-11 Revised:2018-01-12 Published:2018-02-01
  • Corresponding author: Xiaomei Zhang
  • About author:
    Corresponding author: Zhang Xiaomei, Email:
Objective

To investigate gene expression profile of follicular fluid in polycystic ovary syndrome (PCOS) induced infertility patients and non-polycystic ovary syndrome induced infertility patients, in order to clarify possible mechanism about long non-coding(Lnc)RNA in the pathogenesis of PCOS.

Methods

From January 1 to May 30, 2017, a total of 6 follicular fluid samples of infertility patients who underwent assisted reproduction treatment in Northern Jiangsu People′s Hospital, Yangzhou University. They were divided into PCOS group (n=3) and non-PCOS group (n=3) according to whether they had PCOS or not. The age, infertility duration, body mass index (BMI), basic luteinizing hormone (bLH), basic follicle stimulating hormone (bFSH), estradiol, testosterone, fasting blood glucose (FBG), mammotrophic, anti-Mullerian hormone (AMH) were detected by independent-samples t test. The expression of LncRNA and messenger RNA(mRNA) [fragments per kilobase of exon per million fragments mapped (FPKM)], the length of LncRNA and mRNA were both detected by Mann-Whitney U test. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Northern Jiangsu People′s Hospital, Yangzhou University.

Results

① There were no significant difference between two groups in the aspects of age, infertility duration, and the levels of BMI, bLH, bFSH, estradiol, testosterone, FBG, mammotrophic, AMH (P>0.05). ② The median expression levels of LncRNA and mRNA were 0.455 FPKM (0.035-1.291 FPKM) and 0.848 FPKM (0.315-1.735 FPKM), respectively. There was significant difference between them (U=0.041, P<0.05). ③ The median length of LncRNA was 654 bp(499-1 069)bp, which was shorter than that of mRNA[1 517bp (709-2 818 bp)]. There was significant difference between them (U=0.038, P=0.05). ④ Compared with PCOS patients and non-PCOS patients, 1 866 differentially expressed genes were detected, including 1 253 up-regulation genes and 613 down-regulation genes. ⑤ Gene ontology (GO) enrichment analysis showed the scheduling precedence were purine metabolism, purine biosynthesis, cytidine triphosphate biosynthesis, and so on. ⑥ The scheduling precedence of Kyoto Eneyclopedia of Genes and Genomes (KEGG) pathway were human T-lymphotropic virus infection, purine metabolism, pyrimidine metabolism, chemokine, amino acid biosynthesis, asthma, RNA polymerase, Fanconi anemia, which were related with cell biosynthesis, protein synthesis, cell composition synthesis and so on.

Conclusions

The LncRNA and mRNA transcription level changed obviously in both 2 groups, it prompt that follicular fluid of PCOS patients has multiple biological functions and the changes in the signaling pathways which play an important role.

表1 2组患者一般临床资料比较(±s)
图1 LncRNA基因分类图(图1A:基因间LncRNA分类图;图1B:基因内LncRNA分类图;图1C:基因间LncRNA分类模式柱状图;图1D:基因内LncRNA分类模式柱状图)
图2 LncRNA与mRNA表达水平分析图
图3 LncRNA与mRNA的长度分布柱状图(图3A:LncRNA长度;图3B:mRNA 长度)
图4 LncRNA与mRNA的外显子数量分布柱状图(图4A:LncRNA外显子数量;图4B:mRNA 外显子数量)
图5 LncRNA差异表达分析图(图5A:LncRNA差异盒形图;图5B:LncRNA差异火山图)
图6 LncRNA差异聚类分析图
图7 GO富集分析图
图8 KEGG信号通路分析图
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