Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2018, Vol. 14 ›› Issue (01): 77 -84. doi: 10.3877/cma.j.issn.1673-5250.2018.01.013

Special Issue:

Original Article

Study on long non-coding RNA gene expression profile of follicular fluid in polycystic ovary syndrome patients

Liping Wang1, Fang Lyu2, Xueli Li2, Yu Pan2, Xiaoqin Lyu2, Xiao He2, Li Wang3, Xiaomei Zhang2,()   

  1. 1. Department of Biobank, Department of Obstetrics and Gynecology, Clinical Medical School of Yangzhou University·Northern Jiangsu People′s Hospital, Yangzhou University, Yangzhou 225001, Jiangsu Province, China
    2. Reproductive Medicine Center, Department of Obstetrics and Gynecology, Clinical Medical School of Yangzhou University·Northern Jiangsu People′s Hospital, Yangzhou University, Yangzhou 225001, Jiangsu Province, China
    3. John Hopkins School of Medicine, Baltimore 21218, Maryland, United States of America
  • Received:2017-10-11 Revised:2018-01-12 Published:2018-02-01
  • Corresponding author: Xiaomei Zhang
  • About author:
    Corresponding author: Zhang Xiaomei, Email:
Objective

To investigate gene expression profile of follicular fluid in polycystic ovary syndrome (PCOS) induced infertility patients and non-polycystic ovary syndrome induced infertility patients, in order to clarify possible mechanism about long non-coding(Lnc)RNA in the pathogenesis of PCOS.

Methods

From January 1 to May 30, 2017, a total of 6 follicular fluid samples of infertility patients who underwent assisted reproduction treatment in Northern Jiangsu People′s Hospital, Yangzhou University. They were divided into PCOS group (n=3) and non-PCOS group (n=3) according to whether they had PCOS or not. The age, infertility duration, body mass index (BMI), basic luteinizing hormone (bLH), basic follicle stimulating hormone (bFSH), estradiol, testosterone, fasting blood glucose (FBG), mammotrophic, anti-Mullerian hormone (AMH) were detected by independent-samples t test. The expression of LncRNA and messenger RNA(mRNA) [fragments per kilobase of exon per million fragments mapped (FPKM)], the length of LncRNA and mRNA were both detected by Mann-Whitney U test. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Northern Jiangsu People′s Hospital, Yangzhou University.

Results

① There were no significant difference between two groups in the aspects of age, infertility duration, and the levels of BMI, bLH, bFSH, estradiol, testosterone, FBG, mammotrophic, AMH (P>0.05). ② The median expression levels of LncRNA and mRNA were 0.455 FPKM (0.035-1.291 FPKM) and 0.848 FPKM (0.315-1.735 FPKM), respectively. There was significant difference between them (U=0.041, P<0.05). ③ The median length of LncRNA was 654 bp(499-1 069)bp, which was shorter than that of mRNA[1 517bp (709-2 818 bp)]. There was significant difference between them (U=0.038, P=0.05). ④ Compared with PCOS patients and non-PCOS patients, 1 866 differentially expressed genes were detected, including 1 253 up-regulation genes and 613 down-regulation genes. ⑤ Gene ontology (GO) enrichment analysis showed the scheduling precedence were purine metabolism, purine biosynthesis, cytidine triphosphate biosynthesis, and so on. ⑥ The scheduling precedence of Kyoto Eneyclopedia of Genes and Genomes (KEGG) pathway were human T-lymphotropic virus infection, purine metabolism, pyrimidine metabolism, chemokine, amino acid biosynthesis, asthma, RNA polymerase, Fanconi anemia, which were related with cell biosynthesis, protein synthesis, cell composition synthesis and so on.

Conclusions

The LncRNA and mRNA transcription level changed obviously in both 2 groups, it prompt that follicular fluid of PCOS patients has multiple biological functions and the changes in the signaling pathways which play an important role.

表1 2组患者一般临床资料比较(±s)
图1 LncRNA基因分类图(图1A:基因间LncRNA分类图;图1B:基因内LncRNA分类图;图1C:基因间LncRNA分类模式柱状图;图1D:基因内LncRNA分类模式柱状图)
图2 LncRNA与mRNA表达水平分析图
图3 LncRNA与mRNA的长度分布柱状图(图3A:LncRNA长度;图3B:mRNA 长度)
图4 LncRNA与mRNA的外显子数量分布柱状图(图4A:LncRNA外显子数量;图4B:mRNA 外显子数量)
图5 LncRNA差异表达分析图(图5A:LncRNA差异盒形图;图5B:LncRNA差异火山图)
图6 LncRNA差异聚类分析图
图7 GO富集分析图
图8 KEGG信号通路分析图
[1]
hrmann DA.Polycystic ovary syndrome[J].N Engl J Med,2005,352(12):1223-1236.
[2]
Rosenfield RL, Ehrmann DA. The pathogenesis of polycystic ovary syndrome (PCOS): the hypothesis of PCOS as functional ovarian hyperandrogenism revisited[J]. Endocr Rev, 2016, 37(5):467-520.
[3]
Azziz R. PCOS in 2015: New insights into the genetics of polycystic ovary syndrome[J]. Nat Rev Endocrinol, 2016, 12(3):183.
[4]
彭婷婷,苏铁,郑莉萍. 长链非编码RNA在雌性生殖系统中的调节作用[J]. 生理科学进展,2016, 47(4):281-286.
[5]
杨倩,周承亮,潘洁雪,等. 非编码RNA在多囊卵巢综合征中的作用研究进展[J]. 上海交通大学学报(医学版), 2016, 36(6):921.
[6]
Chang J, Azziz R, Legro R, et al. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS)[J]. Fertil Steril, 2004, 19(1):41.
[7]
Ashburner M, Ball CJ, Botstein D, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium[J]. Nature Genetics, 2000, 25(1):25-9.
[8]
Wu J, Mao X, Tao C, et al. KOBAS server: a web-based platform for automated annotation and pathway identification[J]. Nucleic Acids Res, 2006, 34(Web Server issue):w720-w724.
[9]
Lopez-Pajares V. Long non-coding RNA regulation of gene expression during differentiation[J]. Pflugers Arch, 2016, 468(6):971-981.
[10]
Huang X, Hao C, Bao H, et al. Aberrant expression of long noncoding RNAs in cumulus cells isolated from PCOS patients[J]. J Assist Reprod Genet, 2016, 33(1):111-121.
[11]
Quinn JJ, Chang HY. Unique features of long non-coding RNA biogenesis and function[J]. Nat Rev Genet, 2016, 17(1):47-62.
[12]
Abraham JM, Meltzer SJ. Long noncoding RNAs in the pathogenesis of Barrett′s esophagus and esophageal carcinoma[J]. Gastroenterology, 2017, 153(1):27-34.
[13]
王芳,陈绍威. 细胞生长因子微环境与早期胚胎发育[J]. 中国组织工程研究,2016, 20(11):1637-1643.
[14]
宝鲁日,陈秀娟,王丽岩,等. 卵泡液中细胞因子与卵泡发育及妊娠结局的相关性[J]. 国际生殖健康/计划生育杂志,2016, 35(1):47-50.
[15]
Revelli A, Delle Piane L, Casano S, et al. Follicular fluid content and oocyte quality: from single biochemical markers to metabolomics[J]. Reprod Biol Endocrinol, 2009, 7:40.
[16]
Lewandowska AE, Macur K, Czaplewska P, et al. Qualitative and quantitative analysis of proteome and peptidome of human follicular fluid using multiple samples from single donor with LC-MS and SWATH methodology[J]. J Proteome Res, 2017, 16(8):3053-3067.
[17]
Jathar S, Kumar V, Srivastava J, et al. Technological developments in lncRNA biology // Long non coding RNA biology[M]. Singapore: Springer, 2017: 283-323.
[18]
Lagarde J, Uszczynska-Ratajczak B, Santoyo-Lopez J, et al. Extension of human lncRNA transcripts by RACE coupled with long-read high-throughput sequencing (RACE-Seq)[J]. Nat Commun, 2016, 7:12339.
[1] Lu Wang, Yang Fan. Advances in endometrial cancer-related biomarker research[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 511-516.
[2] Ling Wei, Hui Li, Yi Chen. Current research status on colonization or infection and mother-to-child transmission of extended-spectrum β-lactamase-producing Enterobacteriaceae in pregnant and post-partum women[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 517-521.
[3] Dan Luo, Weimin Kong, Shuning Chen, Xiaoling Zhao, Yunkai Xie. Differences in epithelial mesenchymal transition-related biological markers between in-situ and ectopic endometrial epithelial cells in endometriosis patients[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 530-539.
[4] Tiantian Chen, Xiaodong Wang, Haiyan Yu. Pregnancy outcome of twin pregnancy with Gitelman syndrome: a case report and literature review[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 559-568.
[5] Xiaoqing Ju, Yunjie Jin, Xiaoyan Wang. Influencing factors of uterine rupture during vaginal delivery in patients with scarred uterus after cesarean section[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 575-581.
[6] Beibei Wang, Qixiu Dong, Hongyan Xi, Qingyun Yu, Lijun Zhang, Guang Shi. Analysis of influencing factors of medical abortion failure of pregnant women in early pregnancy and construction of related prediction model and its predictive value for medical abortion success[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 588-594.
[7] Xu Chen, Yuru Zhan, Chunhua Wang. Clinical value of ABO blood group combined with thyroid function in prediction of gestational diabetes mellitus[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 604-610.
[8] Mengling Zhou, Zhiwei Xue, Shu Zhou. Changes in size of uterine myoma during pregnancy and its association with adverse pregnancy outcomes[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 611-615.
[9] Dongjie Zhou, Min Jiang, Hairui Fan, Lingling Gao, Xiang Kong, Dan Lu, Liping Wang. Current research progress on non-coding RNA in follicular development and maturation[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(04): 387-393.
[10] Xingchen Liu, Juan Liu, Baobao Wei, Jie Liu, Hui Liu. Correlation analysis of abnormal expression of XIAP and XAF1 with ovarian cancer[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(04): 419-427.
[11] Xiaohui Li, Changsheng Shangguan, Ying Xiang, Zhijie Pei, Junzhi Che, Fei Xie. Relationship between body energy metabolism and sex hormone levels in patients with polycystic ovary syndrome after 3D laparoscopic sleeve gastrectomy[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2023, 17(05): 538-541.
[12] Chufeng Wang, An Jiang. Molecular diagnosis of primary liver cancer[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(05): 499-503.
[13] Feifei Wang, Guanglin Wang, Zesong Meng, Baokun Li, Longfei Cao, Juan Zhang, Chaoxi Zhou, Yuanyi Ding, Guiying Wang. Effect of IMPDH1 knockdown on biological behavior of human colon cancer SW480 and HT29 cells[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(08): 884-890.
[14] Hongqin Gao, Chen Chen, Ruike Lu, Xiaoyu Wang, Min Zhang, Shaohua Li, Lilan Hao, Xincheng Huang, Lingyao Guan, Yunhong Zhang. Impact of vulvovaginal candidiasis on vaginal-cervical microbiota in women[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(06): 720-725.
[15] Xueyun Liu, Ying Fan, Aijun Yao, Shengmiao Zhang, Yani Lv, Bingqing Zhang, Xiaoyu Zhang, Heng. Liu. Effects of individualized whole-course nursing intervention based on WeChat mini program on pregnancy weight and delivery outcome[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(04): 455-460.
Viewed
Full text


Abstract