Influences of Plasma Tumor Necrosis Factor-α, Interleukin-8 and Vascular Endothelial Growth Factor Levels of Neonates with Meconium Aspiration Syndrome Treated by Proportional Assist Ventilation

doi:10.3877/cma.j.issn.1673-5250.2014.02.013

Objective

To investigate influences of tumor necrosis factor-α(TNF-α) , interleukin-8(IL-8) and vascular endothelial growth factor(VEGF) of neonates with meconium aspiration syndrome(MAS) treated by proportional assist ventilation (PAV) .

Methods

From January 2011 to October 2013, a total of 30 neonates with MAS who required mechanical ventilation were recruited into this study．They were divided randomly into two groups according to different ventilation modes PAV group (n=15, supported by PAV mode) and synchronized intermittent mandatory ventilation (SIMV) group (n =15, supported by SIMV mode) ．There had no significant differences between two groups on age, gestation age, birth weight, and so on (P>0.05) ．At the same time, another 10 healthy term infants were selected as control group．The plasma TNF-α, IL-8 and VEGF levels of neonates in three groups were measured by enzyme linked immunosorbent assay at the first day and third day after birth．The study protocol was approved by the Ethical Review Board of Investigation in Huaian Maternity and Child Healthcare Hospital Affiliated to Yangzhou University Medical Academy．Informed consent was obtained from all participates' parents.

Results

There had no statistically significant differences of TNF-α levels among three groups at first day and third day [(1 395.80 ± 426.46) ng/ L vs．(1 428.27 ± 414.24) ng/ L vs．(374.30 ± 229.91) ng/ L; (1 356.87 ± 315.54) ng/ L vs．(1 602.13 ± 371.10) ng/ L vs．(383.30 ± 187.00) ng/ L; F=27.573, 48.142, P=0.000], there also had statistically significant differences of IL-8 levels among three groups at first day and third day [(2.231 ± 1.063) μg/ L vs．(2.409 ± 1.058) μg/ L vs．(0.398 ± 0.214) μg/ L; (1.813 ± 0.701) μg/L vs．(2.484 ± 1.049) μg/ L vs．(0.409 ± 0.231) μg/ L; F=216.198, 21.173, P= 0.000], and there had no statistically significant differences of VEGF levels among three groups at first day and third day [(167.07 ± 77.23) ng/ L vs．(179.93 ± 78.83) ng/ L vs．(41.90 ± 13.51) ng/ L; (141.73 ± 52.69) ng/ L vs．(186.27 ± 68.25) ng/ L vs．(39.10 ± 10.14) ng/ L; F=14.092, 22.987, P=0.000].

Conclusions

In case to maintain normal oxygen saturation and blood gas, PAV mode can reduce the incidence rate of ventilation-induced lung injury than that of SIMV mode.

Key words: Tumor necrosis factor-alpha, Interleukin-8, Vascular endothelial growth factor, Interactive ventilatory support, Meconium aspiration syndrome, Infant, newborn

表1 3组血浆TNF-α水平在不同时间点的比较（ng／L，±s）

Table 1 Comparison of plasma TNF-α levels at different time points among three groups （ng／L，±s）

组别	n	生后1 d	生后3 d	t	P
PAV组	15	1 395.80 ± 426.46^ab	1 356.87 ± 315.54^ac	0.284	0.778
SIMV组	15	1 428.27±414.24^a	1 602.13±371.10^a	-1.208	0.237
对照组	10	374.30 ± 229.91	383.30±187.00	－0.096	0.925
F		27.573	48.142
P		0.000	0.000

表1 3组血浆TNF-α水平在不同时间点的比较（ng／L，±s）

Table 1 Comparison of plasma TNF-α levels at different time points among three groups （ng／L，±s）

组别	n	生后1 d	生后3 d	t	P
PAV组	15	1 395.80 ± 426.46^ab	1 356.87 ± 315.54^ac	0.284	0.778
SIMV组	15	1 428.27±414.24^a	1 602.13±371.10^a	-1.208	0.237
对照组	10	374.30 ± 229.91	383.30±187.00	－0.096	0.925
F		27.573	48.142
P		0.000	0.000

表2 3组血浆IL-8水平在不同时间点的比较（μg／L，±s）

Table 2 Comparison of plasma IL-8 levels at different time points among three groups （μg／L，±s）

组别	n	生后1 d	生后3 d	t	P
PAV组	15	2.231±1.063^ab	1.813±0.701^ac	1.357	0.186
SIMV组	15	2.409 ± 1.058^a	2.484±1.049^a	-0.196	0.846
对照组	10	0.398±0.214	0.409 ± 0.231	-0.111	0.913
F		16.198	21.173
P		0.000	0.000

表2 3组血浆IL-8水平在不同时间点的比较（μg／L，±s）

Table 2 Comparison of plasma IL-8 levels at different time points among three groups （μg／L，±s）

组别	n	生后1 d	生后3 d	t	P
PAV组	15	2.231±1.063^ab	1.813±0.701^ac	1.357	0.186
SIMV组	15	2.409 ± 1.058^a	2.484±1.049^a	-0.196	0.846
对照组	10	0.398±0.214	0.409 ± 0.231	-0.111	0.913
F		16.198	21.173
P		0.000	0.000

表3 3组血浆VEGF水平在不同时间点的比较（ng／L，±s）

Table 3 Comparison of plasma VEGF levels at different time points among three groups(ng／L，±s）

组别	n	生后1 d	生后3 d	t	P
PAV组	15	167.07 ± 77.23^ab	141.73±52.69^ac	1.049	0.303
SIMV组	15	179.93±78.83^a	186.27±68.25^a	-0.235	0.816
对照组	10	41.90±13.51	9.10±10.14	0.524	0.607
F		14.092	22.987
P		0.000	0.000

表3 3组血浆VEGF水平在不同时间点的比较（ng／L，±s）

Table 3 Comparison of plasma VEGF levels at different time points among three groups(ng／L，±s）

组别	n	生后1 d	生后3 d	t	P
PAV组	15	167.07 ± 77.23^ab	141.73±52.69^ac	1.049	0.303
SIMV组	15	179.93±78.83^a	186.27±68.25^a	-0.235	0.816
对照组	10	41.90±13.51	9.10±10.14	0.524	0.607
F		14.092	22.987
P		0.000	0.000

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