Expression and Significance of Vascular Endothelial Growth Factor and Its Receptor Fms-Like Tyrosine Kinase-1 in Placenta for Patients With Intrahepatic Cholestasis of Pregnancy

doi:10.3877/cma.j.issn.1673-5250.2011.06.004

Objective

To investigate the expression and significance of vascular endothelial growth factor (VEGF) and its receptor fms-like tyrosine kinase-1(Flt-1) in placenta for patients with intrahepatic cholestasis of pregnancy (ICP).

Methods

From June 2009 to May 2010, 30 ICP patients (ICP group) and 30 normal third trimester pregnant women (control group) were included in the study. There had no significant differences in age, gestational age, gravidity, parity and modes of delivery between two groups(P>0.05). The expressions of VEGF and Flt-1 in placentas were detected by immunohistochemistry. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Wuxi Hospital for Maternal and Child Health Care.Informed consent was obtained from all participants.

Results

The distribution of VEGF and Flt-1 in placenta were found in villous trophoblast, placental blood vessels, and villous stroma in both groups. The expression levels of VEGF and Flt-1 in placenta in ICP group were significantly lower than those in control group(P<0.01).

Conclusion

The significant decrease of expression levels of VEGF and Flt-1 might be related to inadequate blood vessel formation and the abnormal invasions of trophoblast and might play an important role in the pathogenic mechanism of ICP.

Key words: intrahepatic cholestasis of pregnancy, vascular endothelial growth factor, fms-like tyrosine kinase-1, placenta, pregnant complication

	1　Grundwald M, Avraham I, Dor Y, et al.VEGF－induced adult neovascularization: Recruitment, retention, and role of accessory cells [J]．Cell, 2006，124 (1)：175－189.
	2　Chen R, Zhu ZH, Liu F. Expression of VEGF in non－small cell lung cancer [J]．Jiangsu Med J, 2008, 34(2)：135－136. [陈若，朱之华，刘峰．血管内皮生长因子在非小细胞肺癌中的表达[J]. 江苏医药，2008，34(2)：135－136.]
	3　An RF, Zhou W, Wang S. The expression of VEGF and VEGF receptor (Flt－1) in hydatidiform mole and its significance [J]. Xi'an Jiaotong Univ J(Med Sci), 2004，25(6)：573－575，593. [安瑞芳，周玮，王姝，等．血管内皮生长因子及其受体在正常绒毛及葡萄胎组织中的表达及意义[J]．西安交通大学学报：医学版，2004，25(6)：573－575，593.]
	4　Peng QM, Huang XJ. The application of VEGF in department of gynecology and obstetrics and reproductive field [J]. J Jinggangshan Univ(Natural Sci), 2010，31(4)：91－96. [彭清妹，黄筱金．VEGF在妇产科及生殖领域中的应用[J]．井冈山大学学报：自然科学版，2010，31(4)：91－96.]
	5　Krukier II, Pogorelova TN. Production of vascular endothelial growth factor and endothelin in the placenta and umbilical cord during normal and complicated pregnancy [J]．Bull Exp Biol Med，2006，141(2)：216－218.
	6　Tao YL, Qi HB. Changes and significance of serum and urine VEGF，PLGF and sFlt－1 in preeclampsla pregnant women [J]．J Pract Obstet Gynecol, 2008，24(8)：496－498.[陶娅玲，漆洪波．子痫前期孕妇血清及尿液中VEGF、PLGF、sFlt－1水平变化及意义[J]．实用妇产科杂志，2008，24(8)：496－498.]
	7　Tao L, Cao YX. Study on the correlation of vascular endothelial growth factor with the pathogenesis of intrahepatic cholestasis of pregnancy [J]．Anhui Med J, 2009，30(3)：276－278.[陶丽，曹云霞．血管内皮生长因子与妊娠期肝内胆汁淤积症关系的研究[J]．安徽医学，2009，30(3)：276－278.]
	8　Zhou M, Zhang Y, Zhang CY. Changes and significance of serum VEGF in patients with spontaneous abortion of first－trimester [J]．Shandong Med J, 2010，50(22)：82－83.[周敏，张燕，张呈艳．早期自然流产患者血清VEGF水平变化及意义[J]．山东医药，2010，50(22)：82－83.]
	9　Chen Z, Zhang T, Xu JJ. Expression of vascular endothelial growth factor in patients with fetal growth restriction [J]. Mod J Integrat Trad Chin West Med, 2011，20(5)：528－529. [陈忠，张婷，许建娟．血管内皮生长因子在胎儿生长受限中的表达[J]．现代中西医结合杂志，2011，20(5)：528－529.]

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