Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2011, Vol. 07 ›› Issue (03): 202 -205. doi: 10.3877/cma.j.issn.1673-5250.2011.03.004

Original Article

Practicability Study of Applying High-Risk Human Papilloma Virus E6/E7 mRNA Levels to Determine Cervical Lesion by Using ThinPrep Cytology Test Sample

Tong-yu LIU, Rong XIE, Lulu Zhang, Xiong-wei ZHENG, Gang CHEN, Cheng HE, Yu-zhen LIN, Lin YANG, Sang LI   

  1. Department of Gynecology, Fujian Tumor Hospital, Fuzhou 350014, Fujian Province, China
  • Published:2011-06-01
  • Supported by:
    * Project No. 2009-2-29, supported by the Fund for Young Scholars From Shangdong Provincial Health Department
Objective

To research the feasibility and clinical application value of high-risk human papilloma virus (HR-HPV) E6/E7 mRNA and DNA transcripts in cervical cancer samples by using liquid-based Thin-prep cytology test (TCT).

Methods

Both high-risk HPV E6/E7 mRNA and DNA of 22 samples by two different methods of Specimen handling were detected. The data from detection of high-risk HPV E6/E7 mRNA and HPV E6/E7 DNA in 108 TCT samples with different cervical lesions were statistically analyzed.

Results

Willcoxon rank test was used to exam two different methods of Specimen handling (P> 0.05). The expression level of HPV E6/E7 mRNA transcripts was related to the development of severe epithelial cervical dysplasia. The expression copy number of HPV E6/E7 mRNA transcripts in 108 Thin-prep cytology test was 320.554 ± 779.104. The expression copy number of HPV E6/E7 DNA transcripts in 108 Thin-prep cytology tests was 1540.963 ± 3575.255. The coefficient of Spearman test among HPV DNA and E6/E7 mRNA was 1.

Conclusions

The residual Thin-prep cytology test samples could be used in the detection of high-risk HPV E6/E7 mRNA and HPV E6/E7 DNA. HPV E6 and E7 mRNA test could be a useful tool for cervical cancer screening.

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