Selective Killing Effect of Cytosine Deaminase Gene Controlled by Human Telomerase Reverse Transcriptase Promoter on Cervical Cancer Cells

doi:10.3877/cma.j.issn.1673-5250.2010.03.007

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2010, Vol. 06 ›› Issue (03): 172 -174. doi: 10.3877/cma.j.issn.1673-5250.2010.03.007

Original Article

Selective Killing Effect of Cytosine Deaminase Gene Controlled by Human Telomerase Reverse Transcriptase Promoter on Cervical Cancer Cells

Shu-hua HUANG, Zhi-lan PENG, He WANG, Xiao-yun CHEN, Wei-qiang FENG

Department of Obstetrics and Gynecology, Hexian Memorial Hospital of Panyu, Southern Medical University, Guangzhou 511400, China

Published:2010-06-01

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Objective

To discuss selective killing effects of recombinant adenovirus vectors of cytosine deaminase(CD) gene using a human telomerase reverse transcriptase (hTERT) promoter-driven system on cervical cancer cells.

Methods

Hela cells(study group) and human embryo lung fibroblast (hELF) cells(control group) were separately infected with recombined adenovirus. Then different titers of recombinant adenovirus and different concentrations of 5-flurocytosine acting on Hela cells were performed to measure cell viability and killing effects of adenovirus vector on two groups.

Results

After transfection by recombinant adenovirus Ad-hTERTp-CD in vitro, and combined with 5-flurocytosine, the cell growth of Hela cancer cells(study group) were suppressed compared with human embryo lung fibroblast cells(control group) (P<0.01).

Conclusion

Recombinant adenovirus combined with 5-flurocytosine has a significant killing effect on Hela cells in vitro, but not on human embryo lung fibroblast cells.

Key words: human telomerase reverse transcriptase (hTERT), suicide gene, cytosine deaminase gene, adenovirus vector

图1　Ad-hTERTp-CD/5-FC对Hela细胞的作用

图2　Ad-hTERTp-CD/5-FC对hELF的作用

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[1]	Shu-hua HUANG, Zhi-lan PENG, He WANG. Construction of recombinant adenovirus containing cytosine deaminase gene driven by human telomerase reverse transcriptase promoter[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2007, 03(04): 202-f7.

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Selective Killing Effect of Cytosine Deaminase Gene Controlled by Human Telomerase Reverse Transcriptase Promoter on Cervical Cancer Cells

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