Construction of recombinant adenovirus containing cytosine deaminase gene driven by human telomerase reverse transcriptase promoter

doi:10.3877/cma.j.issn.1673-5250.2007.04.107

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2007, Vol. 03 ›› Issue (04): 202 -f7. doi: 10.3877/cma.j.issn.1673-5250.2007.04.107

Original Article

Construction of recombinant adenovirus containing cytosine deaminase gene driven by human telomerase reverse transcriptase promoter

Shu-hua HUANG, Zhi-lan PENG, He WANG

Hexian Memorial Hospital of Panyu, Panyu 511400, Guangzhou, China

Published:2007-08-01

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Objective

To construct recombinant adenovirus vectors in which the cytosine deaminase (CD) gene is under the control of the human telomerase reverse transcriptase (hTERT) promoter.

Methods

Recombined adenovirus of Ad-hTERTp-CD was constructed using homologous recombination in cells by transforming shuttle vector pSU-Tp-CD and adenovirus backbone vector pBHGE3 into 293 cells.

Results

Recombinant adenovirus vectors were confirmed by PCR analysis and DNA sequencing.

Conclusion

Replication-defective adenovirus vector in which the CD gene was driven by hTERT promoter was made successfully.

Key words: human telomerase reverse transcriptase (hTERT), cytosine deaminase gene (CD gene), adenovirus vector

图1　12号克隆酶切鉴定

图2　PCR扩增CD基因序列

图3　PCR扩增hTERT启动子序列

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[1]

Shu-hua HUANG, Zhi-lan PENG, He WANG, Xiao-yun CHEN, Wei-qiang FENG. [J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2010, 06(03): 172-174.

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Construction of recombinant adenovirus containing cytosine deaminase gene driven by human telomerase reverse transcriptase promoter

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Construction of recombinant adenovirus containing cytosine deaminase gene driven by human telomerase reverse transcriptase promoter