Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2008, Vol. 04 ›› Issue (02): 76 -82. doi: 10.3877/cma.j.issn.1673-5250.2008.02.103

Original Article

An Experimental Study on the Expression of Angiopoietin and Tie2 Receptor in Infantile Hemangioma

Ting WEI, Wen-ying LIU, Yong-juan WEI, Yun-man TANG, Fang ZHOU   

  1. Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu 610041, China
  • Published:2008-04-01
Objective

To investigate the expression of angiopoietin-1(Ang-1), angiopoietin-2 (Ang-2) and Tie2 receptor in human hemangioma xenograft animal model on nude mice.

Methods

The animal model was established by transplanting human hemangioma blocks into nude mice subcutaneously. 12 grafts were harvested on day 56 and 120 after transplantation respectively for fluorescence RT-PCR, Western blot and immunohistochemistry.

Results

The human hemangioma animal model was successfully established. In proliferating and involuting grafts, the level of Ang-1 mRNA was lower than that of the normal foreskins, the level of Ang-2 mRNA and Tie2 mRNA was higher than that of the normal foreskins. Vasculogenesis was found in the grafts.

Conclusion

Ang-1, Ang-2 and Tie2 receptor are abnormally expressed in the human hemangiom xenograft animal model, vasculogenesis exists in the hemangioma grafts which is a primitive form of vessel formation. It is proposed that infantile hemangioma is not a real tumor, but a disorder of vessel formation.

图1 移植瘤Glut1呈强阳性,定位于血管内皮细胞的细胞膜上
图2 人体血管瘤Glut1呈强阳性,定位于血管内皮细胞的细胞膜上
图3 增生期移植瘤Tie2强阳性定位位于血管内皮细胞的细胞膜
图4 消退期移植瘤Tie2呈强阳性,定位于血管内皮细胞的细胞膜
图5 正常包皮组织中Tie2表达较弱
图6 增生期移植瘤Ang-2强阳性表达,定位于血管内皮细胞的细胞浆
图7 消退期移植瘤Ang-2强阳性表达,定位于血管内皮细胞的细胞浆
图8 正常包皮组织中Ang-2表达较弱
图9 增生期移植瘤Ang-1表达很弱
图10 消退期移植瘤Ang-1表达较弱
图11 正常包皮组织中Ang-1有表达,定位于血管内皮细胞的细胞膜和血管周围细胞的细胞浆
图12 血管发生:血管壁由多层内皮细胞所构成,甚至呈实心细胞团块
1 Hiratsuka S, Nakao K, Nakamura K, et al. Membrane fixation of vascular endothelial growth factor receptor 1 ligand-binding domain is important for vasculogenesis and angiogenesis in mice. Mol Cell Biol, 2005, 25(1):346-354.
2 Maisonpierre PC, Suri C, Jones PF, et al. Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science, 1997, 277(5322):55-60.
3 Yamakawa M, Liu LX, Date T, et al. Hypoxia-inducible factor-1 mediates activation of cultured vascular endothelial cells by inducing multiple angiogenic factors. Circ Res, 2003, 93(7):664-673.
4 Papapetropoulos A, Fulton D, Mahboubi K, et al. Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/survivin pathway. J Biol Chem, 2000, 275(13):9102-9105.
5 Stoeltzing O, Ahmad SA, Liu W, et al. Angiopoietin-1 inhibits vascular permeability, angiogenesis, and growth of hepatic colon cancer tumor. Cancer Res, 2003, 63(12):3370-3377.
6 Scharpfenecker M, Fiedler U, Reiss Y, et al. The Tie-2 ligand Angiopoietin-2 destabilizes quiescent endothelium through an internal autocrine loop mechanism. J Cell Sci, 2005, 118:771-780.
7 Zhang L, Yang N, Park JW, et al. Tumor-derived vascular endothelial growth factor up-regulates angiopoietin-2 in host endothelium and destabilizes host vasculature, supporting angiogenesis in ovarian cancer. Cancer Res, 2003, 63(12):3403-3412.
8 Holash J, Maisonpierre PC, Compton D, et al. Vessel cooption, regression, and growth in tumors mediated by angiopoietins and VEGF. Science, 1999, 284(5422):1994-1998.
9 Lobov IB, Brooks PC, Lang RA. Angiopoietin-2 displays VEGF dependent modulation of capillary structure and endothelial cell survival in vivo. Proc Natl Acad Sci USA, 2002, 99(17):11205-11210.
10 Yu Y, Varughese J, Brown LF, et al. Increased tie2 expression, enhanced response to angiopoietin-1, and dysregulated angiopoietin-2 expression in hemangioma-derived endothelial cells. Am J Pathol, 2001, 159(6):2271-2280.
11 徐泉,孟保英,党双锁等.原位RT-PCR检测血管瘤组织Ang-2 mRNA及其受体Tie2 mRNA的表达.第四军医大学学报,2004,25∶979-981.
[1] Jiaxuan Liu, Maiyue He, Qiao Li, Binghe Xu. Application of apatinib in clinical treatment of advanced breast cancer[J]. Chinese Journal of Breast Disease(Electronic Edition), 2023, 17(01): 1-5.
[2] Changsheng Lin, Jun Zhan, Xue Xiao. Genetic testing and precision molecular targeted therapy in diagnosis and treatment of epithelial ovarian caner[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 505-510.
[3] Tao Cao, Ke Tao. Research progress of adipose mesenchymal stem cell exosomes promoting wound angiogenesis[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2022, 17(02): 154-158.
[4] Jianyu Lu, Shichu Xiao, Zhaofan Xia. Research progress of extracellular vesicles derived from keratinocytes in wound healing[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2021, 16(05): 441-444.
[5] Bin Shu, Junyou Zhu, Shaohai Qi. Expression of pigment epithelium-derived factor in wound healing and its effect and mechanism on human dermal microvascular endothelial cells[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2021, 16(04): 301-309.
[6] Shaolong Hao, Wei Han, Yu Ji, Hao Sun, Haowei Shi, Jihong Ma. BANCR promoting lymphangiogenesis by regulating VEGF-C/VEGFR-3 pathway in pancreatic cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2021, 15(04): 269-272.
[7] Yuan Zhang, Xiaolong Li, Yapeng Wang. Relationship between ANGPTL2 protein and immunosuppressive cell infiltration in pancreatic cancer and its clinical significance[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2023, 17(02): 145-148.
[8] Haowei Shi, Shaolong Hao, Yu Ji, Hao Sun, Fang Nie, Yang Hu, Zeqian Li, Wei Han. Expression and clinical significance of long non-coding RNA-BANCR in pancreatic cancer[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2022, 16(05): 554-559.
[9] Junjie Huang, Lie Wang, Hu Zhao, Yin Xia, Zaizhong Zhang. Progress in the mechanism of lncRNA as ceRNA involved in the pathogenesis and progression of infantile hemangioma[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2022, 12(06): 360-366.
[10] Li Ren, Xihua Wu, Ting Liu, Yizhang Mei. Silencing LncRNA MEG3 regulates the protective mechanism of miR-424-5p/FoxO1 against oxidized low-density lipoprotein-induced atherosclerosis[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2022, 12(06): 335-345.
[11] Gonghao Li, Yanli Zhao, Zhongxing Peng, Delu Yin, Yunfeng Zhao. Effect of down-regulation of angiopoietin-like protein 7 expression on AngⅡ-mediated inflammatory response of vascular smooth muscle cells[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2022, 12(02): 93-99.
[12] Enqi Ma, Qian Zhang, Xuemei Chen, Tao Liu. Influence of hematopoietic stem cells induced from iPS cells on endothelial cell angiogenesis and related mechanisms[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2021, 11(04): 193-199.
[13] Jing Zhao, Su Wei, Qingfeng Li, Wei Li. Relationship between serum NLRP3 and ANGPTL4 levels and lower extremity arterial disease in type 2 diabetes mellitus[J]. Chinese Journal of Clinicians(Electronic Edition), 2022, 16(02): 124-130.
[14] Lu Yu, Yonghua Li. Research progress on biological function of mitochondria-associated endoplasmic reticulum membranes and its role in related diseases[J]. Chinese Journal of Diagnostics(Electronic Edition), 2022, 10(04): 284-288.
[15] Peng Zhao, Ping Zhang, Lin Deng, Wei Wu, Xingang Li, Donghai Wang. Effect of direct bypass and combined bypass surgery for adult ischemic Moyamoya disease[J]. Chinese Journal of Cerebrovascular Diseases(Electronic Edition), 2022, 16(01): 38-43.
Viewed
Full text


Abstract