Study on relationship between epidermal growth factor and Bcl-2, Bax genetic expression in placenta of fetal growth retardation

doi:10.3877/cma.j.issn.1673-5250.2007.01.108

Objective

To evaluate the relationship between epidermal growth factor (EGF) and Bcl-2, Bax genetic expression in placenta of fetal growth restriction (FGR).

Methods

25 cases with term normal birth weight and 20 cases with FGR were examined. EGF concentrations in maternal and cord blood were determined by radioimmunoassay (RIA). Bcl-2 and Bax in human placenta were detected by streptavidin peroxidase techniques. Positive cells rate and the ratio of positive area to the total area in single villi were measured by picture analysis system.

Results

Compared to the control group, EGF concentrations of FGR group in both maternal and cord blood decreased significantly (P<0. 01). Bcl-2positive cells rate and the area ratio of positive to villi were both higher in placenta of FGR than that in the normal control group (P < 0. 01). By contrast, Bax both increaded significantly (P<0. 01). In FGR group, there existed positive correlation between EGF concentrations and Bcl-2 expression which included Bcl-2 positive cells rate and the positive area ratio. No relations were observed between EGF and Bax.

Conclusion

The results suggested that reduction of EGF in maternal and cord blood is one of the reason for FGR. Bcl-2 expression is increasing whereas, Bax is decreasing. EGF may inhibit apoptosis in placenta by up-regulating Bcl-2 expression, which may accelerate fetal growth.

Key words: fetal growth retardation, placenta, epidermal growth factor Bcl-2, Bax

图1　 Bcl-2表达呈阳性胞浆胞膜见棕黄色颗粒(IIF×200)

图2　 Bax表达呈阳性胞浆见棕黄色颗粒(IIF×200)

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