Effect of catechin microcapsulation on xanthe oxidase, hydroxy radical and malonydialdehyde in rat with nephrotic syndrome induced-adriblastine

doi:10.3877/cma.j.issn.1673-5250.2006.03.103

Objective

To study the effect of catechin microcapsule on xanthe oxidase(XOD), hydroxy radical(^•OH)and malonydialdehyde(MDA)in rats with nephrotic syndrome induced-adriblastine.

Methods

60 female SD rats are randomly distributed in control group, nephrotic syndrome group, gloucortcoid group, vitamin E group, catechin group and catechin microcapsule group. Nephrotic syndrome rats are caused by injecting adriblastine. before rats were killed, we collect urine, blood, liver tissue and kidney tissue. XOD, ^·OH and MDA concentration were detected by biochemistry assay, 24 hrs urinary protein excretion were detected by Comb's bright blueness assay.

Results

Liver tissue XOD, ^·OH and MDA concentration of rats in microcapsule group were lower than that of in catechin group, gloucortcoid group and vitamin E group, kidney tissue and serum XOD, ^•OH and MDA concentration of rats in microcapsule group were lower than that of in catechin group and vitamin E group, were higher than that of in gloucortcoid group. 24 hrs urinary protein excretion of rats in microcapsule group were lower than that of in catechin group in the end of experiment.

Conclusion

Catechin microcapsule could succeedly reduce 24 hrs urinary protein excretion in rats with nephrotic syndrome induced-adriblastine might be achieved through restraining XOD, eliminating ^•OH efficiency, restraining MDA.

Key words: catechin, microcapsule, nephrotic syndrome, xanthe oxidase, hydroxy radical, lipid peroxidation product malonydialdehyde

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