The effects of fibrin clot and cellular growth factor on the human amniotic epithelial cells

doi:10.3877/cma.j.issn.1673-5250.2006.02.104

Objective

To investigate whether human amniotic epithelial cells(HAEC)could be generate on the matrix of formulated fibrin clot in vitro and the effects of epidermal growth factor(EGF), basic fibroblast growth factor(bFGF)and transforming growth factor β₁(TGF-β₁)on the proliferation of HAEC.

Methods

The cultivated HAECs on the formulated fibrin clot were examined by phase contrast microscopy, Giemsa staining and scanning electron microscopy. We measured apoptosis of HAECs on the formulated fibrin clot with growth factor(EGF,bFGF, TGF-β₁)by TUNEL.

Results

HAECs generate on the formulated fibrin clot in vitro. HAECs exposure to EGF or bFGF was associated with a lower rate of apoptosis than control group(P<0. 05), however, HAECs exposure to TGF-β₁ showed opposite effect(P<0. 05).

Conclusion

HAECs generate well on the formulated fibrin clot in vitro. HAECs exposure to EGF or bFGF was decreased apoptosis rate than control group, however, HAECs exposure to TGF-β₁ was increased effect.

Key words: human amniotic epithelial cells(HAEC), fibrin clot, growth factor

图1　人羊膜上皮细胞在纤维蛋白支架上生长Giemsa染色(× 200)

图2　人羊膜上皮细胞间的细胞接触(×2000)

图3　EGF组细胞凋亡观察(×400)

图4　bFGF组细胞凋亡观察(×400)

图5　TGF-ß₁组细胞凋亡观察(×400)

图6　对照组细胞凋亡观察(×400)

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