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中华妇幼临床医学杂志(电子版)

中华妇幼临床医学杂志(电子版) ›› 2023, Vol. 19 ›› Issue (02) : 242 -248. doi: 10.3877/cma.j.issn.1673-5250.2023.02.017

论著

凉山地区过敏性紫癜患儿人类白细胞抗原-B、-DRB1基因位点多态性研究
石艳†(), 张月, 陈素琼, 王科, 黄兴琼   
  1. 四川省凉山彝族自治州第一人民医院儿科，西昌　615000
  • 收稿日期:2022-11-08 修回日期:2023-03-09 出版日期:2023-04-01
  • 通信作者: 石艳

Human leukocyte antigen-B and -DRB1 loci polymorphism in children with Henoch-Schönlein purpura in Liangshan area

Yan Shi†(), Yue Zhang, Suqiong Chen, Ke Wang, Xingqiong Huang   

  1. Department of Pediatrics, The First People′s Hospital of Liangshan Yi Autonomous Prefecture, Xichang 615000, Sichuan Province, China
  • Received:2022-11-08 Revised:2023-03-09 Published:2023-04-01
  • Corresponding author: Yan Shi
  • Supported by:
    Applied Basic Research Science and Technology Program of Science and Technology Department of Sichuan Province(2021YJ0142)
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引用本文：

石艳, 张月, 陈素琼, 王科, 黄兴琼. 凉山地区过敏性紫癜患儿人类白细胞抗原-B、-DRB1基因位点多态性研究[J]. 中华妇幼临床医学杂志(电子版), 2023, 19(02): 242-248.

Yan Shi, Yue Zhang, Suqiong Chen, Ke Wang, Xingqiong Huang. Human leukocyte antigen-B and -DRB1 loci polymorphism in children with Henoch-Schönlein purpura in Liangshan area[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(02): 242-248.

目的

探讨凉山地区过敏性紫癜(HSP)患儿人类白细胞抗原(HLA)-B、-DRB1基因位点多态性。

方法

选取2021年6月至2022年6月于四川省凉山彝族自治州第一人民医院儿科住院治疗的159例HSP患儿为研究对象，纳入研究组。选择同期于本院体检的132例健康儿童作为对照，纳入对照组。采集2组受试儿外周静脉血3 mL进行HLA基因分型，并采用χ2检验，或连续性校正χ2检验，或Fisher确切概率法，对2组受试儿HLA-B与HLA-DRB1等位基因频率进行统计学比较。本研究遵循的程序符合四川省凉山彝族自治州第一人民医院医学伦理委员会要求，通过该伦理委员会批准(审批文号：凉一医伦理审批〔2021〕2号)，并与所有受试儿家长签署临床研究知情同意书。2组受试儿性别构成比、年龄等一般临床资料分别比较，差异均无统计学意义(P>0.05)。

结果

①2组受试儿HLA-B*07、*08、*13、*15、*27、*35、*37、*38、*39、*40、*41、*44、*46、*48、*51、*52、*54、*55、*56、*57、*58、*59、*67这23种HLA-B等位基因频率分别比较，差异均无统计学意义(P>0.05)。②研究组HSP患儿HLA-DRB1*01等位基因频率为0.9%(3/318)，显著低于对照组的4.5%(12/264)，HLA-DRB1*11等位基因频率为15.1%(48/318)，显著高于对照组的6.1%(16/264)，并且差异均有统计学意义(χ2＝7.45、12.03，P＝0.006、0.001)。2组受试儿HLA-DRB1*03、*04、*07、*08、*09、*10、*12、*13、*14、*15、*16这11种HLA-DRB1等位基因频率比较，差异均无统计学意义(P>0.05)。

结论

凉山地区HSP患儿与健康儿童的HLA-B*07、*08等23种等位基因频率分布基本相似。HLA-DRB1*01等位基因，可能为凉山地区儿童发生HSP的抵抗基因，而HLA-DRB1*11等位基因，则可能为易感基因。

关键词: 紫癜，过敏性, HLA-B抗原, HLA-DR抗原, HLA-B基因, HLA-DRB1基因, 基因位点多态性, 凉山地区, 儿童
Objective

To investigate the human leukocyte antigen (HLA)-B and HLA-DRB1 loci polymorphisms in children with Henoch-Schönlein purpura (HSP) in Liangshan area.

Methods

A total of 159 cases of HSP children who were admitted to the Department of Pediatrics, The First People′s Hospital of Liangshan Yi Autonomous Prefecture from June 2021 to June 2022 were selected into this study and enrolled into study group. Meanwhile, 132 cases of healthy children who underwent physical examinations during the same period in the same hospital were enrolled as control group. Peripheral venous blood samples (3 mL) were collected from children in both groups for HLA gene typing, and the allele frequencies of HLA-B and HLA-DRB1 genes were statistically compared by chi-square test, continuity adjusted chi-square test, or Fisher′s exact probability method. The study protocol was approved by the Medical Ethics Committee of The First People′s Hospital of Liangshan Yi Autonomous Prefecture (Approval No.[2021]2), and informed consent was obtained from the parents of all participants. There were no statistically significant differences in composition ratio of gender and age between two groups (P>0.05).

Results

①There were no statistically significant differences in frequencies of 23 kinds of HLA-B allele genes, such as HLA-B*07, HLA-B*08, HLA-B*13, HLA-B*15, HLA-B*27, HLA-B*35, HLA-B*37, HLA-B*38, HLA-B*39, HLA-B*40, HLA-B*41, HLA-B*44, HLA-B*46, HLA-B*48, HLA-B*51, HLA-B*52, HLA-B*54, HLA-B*55, HLA-B*56, HLA-B*57, HLA-B*58, HLA-B*59, HLA-B*67 between two groups (P>0.05). ②The frequency of HLA-DRB1*01 allele gene in study group was 0.9% (3/318), which was significantly lower than that in control group (4.5%, 12/264), while the frequency of HLA-DRB1*11 allele gene in study group was 15.1% (48/318), which was significantly higher than that in control group (6.1%, 16/264), and both the differences between two groups were statistically significant (χ2=7.45, 12.03; P=0.006, 0.001). There were no statistically significant differences in frequencies of other HLA-DRB1 allele genes, such as HLA-DRB1*03, HLA-DRB1*04, HLA-DRB1*07, HLA-DRB1*08, HLA-DRB1*09, HLA-DRB1*10, HLA-DRB1*12, HLA-DRB1*13, HLA-DRB1*14, HLA-DRB1*15, HLA-DRB1*16 between two groups (P>0.05).

Conclusions

The distribution of 23 kinds of HLA-B allele genes including HLA-B*07, HLA-B*08 and so on of HSP children in Liangshan area is similar to that in healthy children. HLA-DRB1*01 allele gene may be a resistant gene in HSP children in Liangshan area, while HLA-DRB1*11 allele gene may be its susceptible gene.

Key words: Purpura, Schoenlein-Henoch, HLA-B antigens, HLA-DR antigens, HLA-B gene, HLA-DRB1 gene, Gene polymorphism, Liangshan area, Child
表1 2组受试儿一般临床资料比较
组别 例数 性别[例数(%)] 年龄(岁，±s)
男 女
研究组 159 84(52.8) 75(47.2) 8.3±1.9
对照组 132 70(53.0) 62(47.0) 8.6±2.3
统计量   χ2<0.01 t＝1.22
P值   0.973 0.224
表2 2组受试儿HLA-B等位基因频率比较[例数(%)]
组别 例数 HLA-B*07 HLA-B*08 HLA-B*13 HLA-B*15 HLA-B*27 HLA-B*35 HLA-B*37 HLA-B*38
研究组 159 12(3.8) 1(0.3) 18(5.7) 57(17.9) 11(3.5) 16(5.0) 4(1.3) 10(3.1)
对照组 132 14(5.3) 1(0.4) 13(4.9) 43(16.3) 9(3.4) 7(2.7) 4(1.5) 12(4.5)
χ2值   0.79 —a 0.16 0.27 <0.01 2.15 0.01b 0.78
P值   0.374 0.500 0.694 0.602 0.974 0.142 0.920 0.378
组别 例数 HLA-B*39 HLA-B*40 HLA-B*41 HLA-B*44 HLA-B*46 HLA-B*48 HLA-B*51 HLA-B*52
研究组 159 13(4.1) 52(16.4) 0(0) 3(0.9) 23(7.2) 6(1.9) 33(10.4) 16(5.0)
对照组 132 6(2.3) 39(14.8) 1(0.4) 3(1.1) 25(9.5) 5(1.9) 30(11.4) 10(3.8)
χ2值   1.51 0.27 —a 0.03b 0.95 0.09b 0.15 0.52
P值   0.220 0.601 0.454 0.862 0.329 0.764 0.703 0.470
组别 例数 HLA-B*54 HLA-B*55 HLA-B*56 HLA-B*57 HLA-B*58 HLA-B*59 HLA-B*67
研究组 159 8(2.5) 17(5.3) 3(0.9) 0(0) 14(4.4) 0(0) 1(0.3)
对照组 132 7(2.7) 10(3.8) 1(0.4) 2(0.8) 19(7.2) 1(0.4) 2(0.8)
χ2值   0.01 0.79 0.10b —a 2.11 —a 0.03
P值   0.918 0.374 0.751 0.205 0.147 0.454 0.871
表3 2组受试儿HLA-DRB1等位基因频率比较[例数(%)]
组别 例数 HLA-DRB1*01 HLA-DRB1*03 HLA-DRB1*04 HLA-DRB1*07 HLA-DRB1*08 HLA-DRB1*09 HLA-DRB1*10
研究组 159 3(0.9) 9(2.8) 59(18.6) 4(1.3) 26(8.2) 32(10.1) 4(1.3)
对照组 132 12(4.5) 11(4.2) 36(13.6) 8(3.0) 29(11.0) 31(11.7) 7(2.7)
χ2值   7.45 0.78 2.55 2.24 1.33 0.43 0.85a
P值   0.006 0.378 0.110 0.134 0.249 0.516 0.356
组别 例数 HLA-DRB1*11 HLA-DRB1*12 HLA-DRB1*13 HLA-DRB1*14 HLA-DRB1*15 HLA-DRB1*16
研究组 159 48(15.1) 59(18.6) 8(2.5) 34(10.7) 26(8.2) 6(1.9)
对照组 132 16(6.1) 42(15.9) 8(3.0) 24(9.1) 30(11.4) 10(3.8)
χ2值   12.03 0.70 0.14 0.41 1.69 1.95
P值   0.001 0.402 0.705 0.521 0.194 0.163
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