切换至 "中华医学电子期刊资源库"

中华妇幼临床医学杂志(电子版) ›› 2023, Vol. 19 ›› Issue (02) : 242 -248. doi: 10.3877/cma.j.issn.1673-5250.2023.02.017

论著

凉山地区过敏性紫癜患儿人类白细胞抗原-B、-DRB1基因位点多态性研究
石艳(), 张月, 陈素琼, 王科, 黄兴琼   
  1. 四川省凉山彝族自治州第一人民医院儿科,西昌 615000
  • 收稿日期:2022-11-08 修回日期:2023-03-09 出版日期:2023-04-01
  • 通信作者: 石艳

Human leukocyte antigen-B and -DRB1 loci polymorphism in children with Henoch-Schönlein purpura in Liangshan area

Yan Shi(), Yue Zhang, Suqiong Chen, Ke Wang, Xingqiong Huang   

  1. Department of Pediatrics, The First People′s Hospital of Liangshan Yi Autonomous Prefecture, Xichang 615000, Sichuan Province, China
  • Received:2022-11-08 Revised:2023-03-09 Published:2023-04-01
  • Corresponding author: Yan Shi
  • Supported by:
    Applied Basic Research Science and Technology Program of Science and Technology Department of Sichuan Province(2021YJ0142)
引用本文:

石艳, 张月, 陈素琼, 王科, 黄兴琼. 凉山地区过敏性紫癜患儿人类白细胞抗原-B、-DRB1基因位点多态性研究[J/OL]. 中华妇幼临床医学杂志(电子版), 2023, 19(02): 242-248.

Yan Shi, Yue Zhang, Suqiong Chen, Ke Wang, Xingqiong Huang. Human leukocyte antigen-B and -DRB1 loci polymorphism in children with Henoch-Schönlein purpura in Liangshan area[J/OL]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(02): 242-248.

目的

探讨凉山地区过敏性紫癜(HSP)患儿人类白细胞抗原(HLA)-B、-DRB1基因位点多态性。

方法

选取2021年6月至2022年6月于四川省凉山彝族自治州第一人民医院儿科住院治疗的159例HSP患儿为研究对象,纳入研究组。选择同期于本院体检的132例健康儿童作为对照,纳入对照组。采集2组受试儿外周静脉血3 mL进行HLA基因分型,并采用χ2检验,或连续性校正χ2检验,或Fisher确切概率法,对2组受试儿HLA-BHLA-DRB1等位基因频率进行统计学比较。本研究遵循的程序符合四川省凉山彝族自治州第一人民医院医学伦理委员会要求,通过该伦理委员会批准(审批文号:凉一医伦理审批〔2021〕2号),并与所有受试儿家长签署临床研究知情同意书。2组受试儿性别构成比、年龄等一般临床资料分别比较,差异均无统计学意义(P>0.05)。

结果

①2组受试儿HLA-B*07、*08、*13、*15、*27、*35、*37、*38、*39、*40、*41、*44、*46、*48、*51、*52、*54、*55、*56、*57、*58、*59、*67这23种HLA-B等位基因频率分别比较,差异均无统计学意义(P>0.05)。②研究组HSP患儿HLA-DRB1*01等位基因频率为0.9%(3/318),显著低于对照组的4.5%(12/264),HLA-DRB1*11等位基因频率为15.1%(48/318),显著高于对照组的6.1%(16/264),并且差异均有统计学意义(χ2=7.45、12.03,P=0.006、0.001)。2组受试儿HLA-DRB1*03、*04、*07、*08、*09、*10、*12、*13、*14、*15、*16这11种HLA-DRB1等位基因频率比较,差异均无统计学意义(P>0.05)。

结论

凉山地区HSP患儿与健康儿童的HLA-B*07、*08等23种等位基因频率分布基本相似。HLA-DRB1*01等位基因,可能为凉山地区儿童发生HSP的抵抗基因,而HLA-DRB1*11等位基因,则可能为易感基因。

Objective

To investigate the human leukocyte antigen (HLA)-B and HLA-DRB1 loci polymorphisms in children with Henoch-Schönlein purpura (HSP) in Liangshan area.

Methods

A total of 159 cases of HSP children who were admitted to the Department of Pediatrics, The First People′s Hospital of Liangshan Yi Autonomous Prefecture from June 2021 to June 2022 were selected into this study and enrolled into study group. Meanwhile, 132 cases of healthy children who underwent physical examinations during the same period in the same hospital were enrolled as control group. Peripheral venous blood samples (3 mL) were collected from children in both groups for HLA gene typing, and the allele frequencies of HLA-B and HLA-DRB1 genes were statistically compared by chi-square test, continuity adjusted chi-square test, or Fisher′s exact probability method. The study protocol was approved by the Medical Ethics Committee of The First People′s Hospital of Liangshan Yi Autonomous Prefecture (Approval No.[2021]2), and informed consent was obtained from the parents of all participants. There were no statistically significant differences in composition ratio of gender and age between two groups (P>0.05).

Results

①There were no statistically significant differences in frequencies of 23 kinds of HLA-B allele genes, such as HLA-B*07, HLA-B*08, HLA-B*13, HLA-B*15, HLA-B*27, HLA-B*35, HLA-B*37, HLA-B*38, HLA-B*39, HLA-B*40, HLA-B*41, HLA-B*44, HLA-B*46, HLA-B*48, HLA-B*51, HLA-B*52, HLA-B*54, HLA-B*55, HLA-B*56, HLA-B*57, HLA-B*58, HLA-B*59, HLA-B*67 between two groups (P>0.05). ②The frequency of HLA-DRB1*01 allele gene in study group was 0.9% (3/318), which was significantly lower than that in control group (4.5%, 12/264), while the frequency of HLA-DRB1*11 allele gene in study group was 15.1% (48/318), which was significantly higher than that in control group (6.1%, 16/264), and both the differences between two groups were statistically significant (χ2=7.45, 12.03; P=0.006, 0.001). There were no statistically significant differences in frequencies of other HLA-DRB1 allele genes, such as HLA-DRB1*03, HLA-DRB1*04, HLA-DRB1*07, HLA-DRB1*08, HLA-DRB1*09, HLA-DRB1*10, HLA-DRB1*12, HLA-DRB1*13, HLA-DRB1*14, HLA-DRB1*15, HLA-DRB1*16 between two groups (P>0.05).

Conclusions

The distribution of 23 kinds of HLA-B allele genes including HLA-B*07, HLA-B*08 and so on of HSP children in Liangshan area is similar to that in healthy children. HLA-DRB1*01 allele gene may be a resistant gene in HSP children in Liangshan area, while HLA-DRB1*11 allele gene may be its susceptible gene.

表1 2组受试儿一般临床资料比较
表2 2组受试儿HLA-B等位基因频率比较[例数(%)]
表3 2组受试儿HLA-DRB1等位基因频率比较[例数(%)]
[1]
Barnhart ML, Rosenbaum K. Anaphylactoid syndrome of pregnancy[J]. Nurs Womens Health, 2019, 23(1): 38-48. DOI: 10.1016/j.nwh.2018.11.006.
[2]
王庆谊,孟昭影. 过敏性紫癜发病机制的研究进展[J]. 中国中西医结合皮肤性病学杂志2020, 19(3): 285-287. DOI: 10.3969/j.issn.1672-0709.2020.03.028.
[3]
杜娟,姚新生,于红松. HLA基因与常见自身免疫病相关性研究进展[J]. 中国免疫学杂志2019, 35(1): 118-122, 128. DOI: 10.3969/j.issn.1000-484X.2019.01.025.
[4]
López-Mejías R, Castañeda S, Genre F, et al. Genetics of immunoglobulin-A vasculitis (Henoch-Schönlein purpura): an updated review[J]. Autoimmun Rev, 2018, 17(3): 301-315. DOI: 10.1016/j.autrev.2017.11.024.
[5]
王天有, 申昆玲, 沈颖,等. 诸福棠实用儿科学[M]. 9版. 北京:人民卫生出版社,2022: 875.
[6]
He X, Yu C, Zhao P, et al. The genetics of Henoch-Schönlein purpura: a systematic review and Meta-analysis[J]. Rheumatol Int, 2013, 33(6): 1387-1395. DOI: 10.1007/s00296-012-2661-4.
[7]
Hetland LE, Susrud KS, Lindahl KH,et al. Henoch-Schönlein purpura: a literature review[J]. Acta Derm Venereol, 2017, 97(10): 1160-1166. DOI: 10.2340/00015555-2733.
[8]
Oni L, Sampath S. Childhood IgA vasculitis (Henoch Schonlein purpura)-advances and knowledge gaps[J]. Front Pediatr, 2019, 7: 257. DOI: 10.3389/fped.2019.00257.
[9]
Reid-Adam J. Henoch-Schonlein purpura[J]. Pediatr Rev, 2014, 35(10): 447-449, discussion 449. DOI: 10.1542/pir.35-10-447.
[10]
孙小妹,戴亮,孙飞扬,等. 成都地区与川西高原地区过敏性紫癜患儿临床特征比较分析[J]. 现代预防医学2018, 45(12): 2274-2276, 2287.
[11]
李克莉,刘大卫,武文娣,等. 中国六个市2007~2009年过敏性紫癜住院病例发病情况分析[J]. 中国疫苗和免疫2011, 17(2): 128-132.
[12]
Wei CC, Lin CL, Shen TC, et al. Atopic dermatitis and association of risk for Henoch-Schönlein purpura (IgA vasculitis) and renal involvement among children: results from a population-based cohort study in Taiwan[J]. Medicine (Baltimore), 2016, 95(3): e2586. DOI: 10.1097/MD.0000000000002586.
[13]
钟芳芳,杨友,郭铃,等. IL-17A基因多态性与过敏性紫癜易感性及紫癜性肾炎的相关性研究[J]. 川北医学院学报2022, 37(1): 1-5. DOI: 10.3969/j.issn.1005-3697.2022.01.001.
[14]
全湛柔,邓志辉,周丹,等. 中国南方汉族造血干细胞捐献者HLA-A、-B、-C、-DRB1及-DQB1高分辨等位基因多态性分析[J]. 国际输血及血液学杂志2018, 41(6): 497-505. DOI: 10.3760/cma.j.issn.1673-419X.2018.06.007.
[15]
朱传福,聂向民,宋永红,等. 中国造血干细胞捐献人群37个HLA新等位基因形成的分子机制分析[J]. 国际输血及血液学杂志2022, 45(1): 68-73. DOI: 10.3760/cma.j.cn511693-20200916-00206.
[16]
金士正,邹红岩,甄建新,等. 中国南方汉族人群HLA-Ⅰ、Ⅱ类八个位点等位基因多态性的研究[J]. 中华医学遗传学杂志2017, 34(1): 110-114. DOI: 10.3760/cma.j.issn.1003-9406.2017.01.026.
[17]
Nyulassy S, Buc M, Sasinka M, et al. The HLA system in glomerulonephritis[J]. Clin Immunol Immunopathol, 1977, 7(3): 319-323. DOI: 10.1016/0090-1229(77)90064-2.
[18]
Rashidi S, Shiari R, Farivar S. HLA-DRB1 gene polymorphisms in Iranian children with Henoch-Schönlein purpura[J]. J Res Med Sci, 2018, 23: 42. DOI: 10.4103/jrms.JRMS_344_17.
[19]
闫亚飞. 宁夏地区回汉儿童HLA基因多态性研究及其与体质和紫癜的相关性[D]. 银川:宁夏医科大学,2017.
[20]
López-Mejías R, Genre F, Pérez BS, et al. Association of HLA-B*41:02 with Henoch-Schönlein purpura (IgA vasculitis) in Spanish individuals irrespective of the HLA-DRB1 status[J]. Arthritis Res Ther, 2015, 17(1):102-108. DOI: 10.1002/art.38979.
[21]
任少敏,杨光路,刘春枝,等. 内蒙地区蒙汉族儿童过敏性紫癜HLA-AB关联基因的探讨及测序分析[J]. 现代免疫学2009, 29(1): 68-70.
[22]
贺少军,陶仲宾,凌继祖,等. HLA基因与过敏性紫癜及其肾脏损害遗传易感性的研究进展[J]. 医学综述2022, 28(10): 1901-1905. DOI: 10.3969/j.issn.1006-2084.2022.10.006.
[1] 陶宏宇, 叶菁菁, 俞劲, 杨秀珍, 钱晶晶, 徐彬, 徐玮泽, 舒强. 右心声学造影在儿童右向左分流相关疾病中的评估价值[J/OL]. 中华医学超声杂志(电子版), 2024, 21(10): 959-965.
[2] 刘琴, 刘瀚旻, 谢亮. 基质金属蛋白酶在儿童哮喘发生机制中作用的研究现状[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(05): 564-568.
[3] 向韵, 卢游, 杨凡. 全氟及多氟烷基化合物暴露与儿童肥胖症相关性研究现状[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(05): 569-574.
[4] 王雅楠, 刘丹, 曹正浓, 贾慧敏. 儿童迟发性先天性膈疝患儿的临床诊治特点分析[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(04): 410-419.
[5] 陈桂华, 钟小玲, 谢雨, 王慧, 谢江, 杨涛毅. 合并肝脏疾病特殊健康状态儿童疫苗预防接种及时性临床分析[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(04): 431-439.
[6] 雷丽莉, 于晓峰, 王媛媛, 徐迎军. 人鼻病毒感染喘息急性发作儿童外周血NLRP3和TLR4水平及临床意义[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(04): 440-445.
[7] 顾盼盼, 董传莉, 宋梦瑶, 瞿色华, 杨小迪, 周瑞. 不完全性川崎病患儿临床特征及冠状动脉损害情况分析[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(04): 446-451.
[8] 刘冉佳, 崔向丽, 周效竹, 曲伟, 朱志军. 儿童肝移植受者健康相关生存质量评价的荟萃分析[J/OL]. 中华移植杂志(电子版), 2024, 18(05): 302-309.
[9] 丁荷蓓, 王珣, 陈为国. 七氟烷吸入麻醉与异丙酚静脉麻醉在儿童腹股沟斜疝手术中的应用比较[J/OL]. 中华疝和腹壁外科杂志(电子版), 2024, 18(05): 570-574.
[10] 曾纪晓, 徐晓钢, 王欣星, 刘斐, 兰梦龙, 陶波圆, 梁子建, 叶志华, 罗媛圆. 达芬奇机器人辅助Swenson-like巨结肠根治术[J/OL]. 中华腔镜外科杂志(电子版), 2024, 17(04): 239-243.
[11] 中华医学会器官移植学分会, 中华医学会外科学分会外科手术学学组, 中华医学会外科学分会移植学组, 华南劈离式肝移植联盟. 劈离式供肝儿童肝移植中国临床操作指南[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 593-601.
[12] 刘军, 丘文静, 孙方昊, 李松盈, 易述红, 傅斌生, 杨扬, 罗慧. 在体与离体劈离式肝移植在儿童肝移植中的应用比较[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 688-693.
[13] 张琛, 秦鸣, 董娟, 陈玉龙. 超声检查对儿童肠扭转缺血性改变的诊断价值[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(06): 565-568.
[14] 陈晓胜, 何佳, 刘方, 吴蕊, 杨海涛, 樊晓寒. 直立倾斜试验诱发31 秒心脏停搏的植入心脏起搏器儿童一例并文献复习[J/OL]. 中华脑血管病杂志(电子版), 2024, 18(05): 488-494.
[15] 曹亚丽, 高雨萌, 张英谦, 李博, 杜军保, 金红芳. 儿童坐位不耐受的临床进展[J/OL]. 中华脑血管病杂志(电子版), 2024, 18(05): 510-515.
阅读次数
全文


摘要