探讨弗林蛋白酶(furin)在正常宫颈组织与宫颈上皮内瘤变(CIN)组织中的表达水平及其与CIN病理分级的关系。

选取2015年7月至2016年7月，于唐山市妇幼保健院妇科门诊进行阴道镜下宫颈活组织病理学检查，并确诊为CIN的68例患者为研究对象，纳入CIN组。选取同期宫颈液基细胞学检查结果为非典型鳞状细胞，于本院妇科门诊进行阴道镜下宫颈活组织病理学检查，而确诊为正常子宫颈的20例受试者，纳入对照组。采集2组受试者宫颈组织标本，采用实时荧光定量PCR法检测其furin mRNA相对表达水平，Western blotting法检测furin相对表达水平。对2组受试者宫颈组织furin mRNA及furin相对表达水平、furin阳性表达率进行统计学比较。本研究遵循的程序符合唐山市妇幼保健院伦理委员会制定的标准，得到该伦理委员会批准，并与所有受试者均签署临床研究知情同意书。2组受试者的年龄等一般临床资料比较，差异均无统计学意义(P>0.05)。

①CIN组受试者宫颈组织furin mRNA相对表达水平为1.59±0.74，显著高于对照组的0.63±0.08，2组比较，差异有统计学意义(t＝2.51，P＝0.004)。②CIN组受试者宫颈组织furin相对表达水平为1.29±0.54，显著高于对照组的0.15±0.04，2组比较，差异有统计学意义(t＝2.09，P＝0.015)。③CIN组受试者宫颈组织furin阳性表达率为63.2%(43/68)，显著高于对照组的15.0%(3/20)，2组比较，差异有统计学意义(χ² ＝14.41，P<0.001)。在CIN组CIN1～3宫颈组织及对照组正常宫颈组织中，furin阳性表达率分别为31.6%(6/19)、65.2%(15/23)、84.6%(22/26)及15.0%(3/20)，furin阳性表达率随着CIN病理分级增高而增高，并且差异有统计学意义(χ² ＝26.56，P＝0.003)。

Furin可能参与了CIN的发生、发展，其高表达可能是反映CIN的早期指标。Furin有望成为早期预测CIN的分子标志物。

To investigate the expression of furin in normal cervical tissues and cervical intraepithelial neoplasia (CIN) tissues, and to explore its relationship with the clinical pathological stages of CIN.

A total of 68 cases of CIN patients who were diagnosed by cervical biopsy guided by colposcopy in the Department of Gynecology, Tangshan Maternal and Child Health Hospital from July 2015 to July 2016 were selected as CIN group. And 20 cases who were diagnosed as normal cervix by cervical biopsy guided by colposcopy in the same hospital during the same period due to atypical squamous cells in the cervical liquid based cytology examination were included into control group. Cervical tissue specimens of two groups were collected, and the relative expression levels of furin mRNA were detected by real-time fluorescence quantitative PCR, and the relative expression levels of furin were detected by Western blotting method. The relative expression levels of furin mRNA and furin, and the positive expressions rate of furin in two groups were compared by statistical methods. The following procedures were in line with the standards set by the Ethics Committee of Tangshan Maternal and Child Health Hospital and approved by the committee. All subjects in the clinical study signed the informed consents. There were no statistically significant differences between two groups in the age and other general clinical data (P>0.05).

①The relative expression level of furin mRNA in the cervical tissue of CIN group was 1.59±0.74, which was statistically significant higher than that of 0.63±0.08 in control group, and the difference was statistically significant (t=2.51, P=0.004). ②The relative expression level of furin in the cervical tissue of CIN group was 1.29±0.54, which was statistically significant higher than that of 0.15±0.04 in control group, and the difference was statistically significant (t=2.09, P=0.015). ③In CIN group, the positive expression rate of furin was 63.2% (43/68), which was significantly higher than that of 15.0% (3/20) in control group, and the difference was statistically significant (χ²=14.41, P<0.001). In CIN1-3 cervical tissues of CIN group and normal cervical tissues of control group, positive expression rate of furin was 31.6% (6/19), 65.2% (15/23), 84.6% (22/26), 15.0% (3/20), respectively, the positive expression rate of furin increased with the increasing clinical pathological stage of CIN, and the difference was statistically significant (χ²=26.56, P=0.003).

Furin may participates in the occurrence and development of CIN, and high level of furin expression may be an early indicator of CIN. Furin is expected to be a molecular marker for early prediction of CIN.