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中华妇幼临床医学杂志(电子版) ›› 2015, Vol. 11 ›› Issue (4) : 492 -496. doi: 10.3877/cma.j.issn.1673-5250.2015.04.012

所属专题: 文献

论著

白细胞介素-17在宫颈癌组织中的表达及其临床意义
陈诗雨, 全懿, 曾玺, 郄明蓉*()   
  1. 610041 成都,四川大学华西第二医院妇产科
  • 收稿日期:2014-12-10 修回日期:2015-06-01 出版日期:2015-08-01
  • 通信作者: 郄明蓉

Expression and significance of interleukin-17 in cervical cancer

Shiyu Chen, Yi Quan, Xi Zeng, Mingrong Qie*()   

  1. Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2014-12-10 Revised:2015-06-01 Published:2015-08-01
  • Corresponding author: Mingrong Qie
引用本文:

陈诗雨, 全懿, 曾玺, 郄明蓉. 白细胞介素-17在宫颈癌组织中的表达及其临床意义[J]. 中华妇幼临床医学杂志(电子版), 2015, 11(4): 492-496.

Shiyu Chen, Yi Quan, Xi Zeng, Mingrong Qie. Expression and significance of interleukin-17 in cervical cancer[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2015, 11(4): 492-496.

目的

探讨白细胞介素(IL)-17在宫颈癌组织中的表达及其与临床病理特征间的关系。

方法

选择2011年1月至2013年1月于四川大学华西第二医院妇科住院接受手术治疗的宫颈癌(术后均经组织病理学检查结果证实为宫颈鳞状细胞癌)患者的石蜡标本61例为研究对象,并纳入研究组。选择同期在本院因子宫良性病变(子宫肌瘤、子宫腺肌症等)接受手术切除的正常宫颈组织的石蜡标本9例纳入对照组。应用链霉亲和素-生物素复合物免疫组织化学(SABC-IHC)染色法对两组标本进行染色,分析IL-17在两组中的表达情况和分布特点,以及IL-17表达与不同临床病理特征间的关系。

结果

①IL-17在宫颈癌组织中主要表达在癌细胞的细胞质内。②IL-17在对照组中无阳性表达,在研究组中阳性表达率为54.1%,二者比较,差异有统计学意义(χ2=7.17,P<0.01)。③IL-17在宫颈癌术后脉管癌栓呈阳性者中的阳性表达率(66.7%)显著高于呈阴性者(39.3%),二者比较,差异有统计学意义(χ2=4.57,P<0.05)。IL-17在宫颈癌国际妇产科联盟(FIGO)临床分期为Ⅱ期患者中的阳性表达率(66.7%)显著高于Ⅰ期者(36.0%),并且差异有统计学意义(χ2=5.59,P<0.05)。

结论

IL-17表达与宫颈癌的发展、浸润、转移相关,对宫颈癌临床诊断及预后评估有重要意义。

Objective

To evaluate the expression of interleukin (IL)-17 in cervical cancer and analyze the association between the expression of IL-17 and its different clinical characteristics.

Methods

From January 2011 to January 2013, a total of 61 samples of cervical cancer (cervical squamous cell carcinoma) tissuse were enrolled into study group. At the same time, another 9 case of samples from benign uterine disease (uterine fibroids, adenomyosis) were enrolled into control group. Expression levels of IL-17 in the 9 cases paraffin sections of normal cervical tissues and 61 cases paraffin sections of cervical cancer were detected by streptavidin-biotin complex immunohistochemistry (SABC-IHC). The relationship between the IL-17 expression and its different clinical characteristics were analyzed by statistical method.

Results

①IL-17 expressed mainly in the cytoplasm of cervical cancer cells. ②The positive rate of IL-17 expression in study group was 54.1%. The difference of positive rate of IL-17 expression in two groups was significant (χ2=7.17, P<0.01). ③The positive rate of IL-17 expression was significantly higher in patients with peritumor intravascular cancer emboli (66.7%) than those without peritumor intravascular cancer emboli (39.3%), and the difference was significantly different (χ2=4.57, P=0.05); the positive rate of IL-17 expression in patients with International Federation of Gynecology and Obstetrics (FIGO) stage Ⅱ was significantly higher than those with FIGO stage Ⅰ, and the difference was significantly different (χ2=5.59, P=0.05).

Conclusions

The expression of IL-17 was associated with the development, infiltration and metastasis of cervical cancer. IL-17 may be an important factor for clinical diagnosis and prognosis of cervical cancer.

表1 两组IL-17表达情况比较[例数(%)]
表2 研究组患者IL-17表达与宫颈癌不同临床病理特征间的关系[例数(%)]
图1 两组标本免疫组织化学染色结果比较(图1A:IL-17在宫颈癌组织中表达呈阴性,SABC-IHC染色,×200;图1B:IL-17在宫颈癌组织中表达呈阳性,SABC-IHC染色,×200;图1C:IL-17在正常宫颈组织中表达呈阴性,SABC-IHC染色,×200;图1D:IL-17在宫颈癌组织中表达呈阴性,SABC-IHC染色,×400;图1E:IL-17在宫颈癌组织表达呈阳性,SABC-IHC染色,×400;图1F:IL-17在正常宫颈组织中表达呈阴性,SABC-IHC染色,×400)
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