同种异体Sprague-Dawley大鼠子宫内膜异位症动物模型的建立

doi:10.3877/cma.j.issn.1673-5250.2015.03.022

目的

探讨采用皮下移植法建立大鼠同种异体子宫内膜异位症(EMS)模型的可行性。

方法

选择健康、未交配且处于动情期(正常动情周期≥2个)的雌性Sprague-Dawley(SD)大鼠48只为研究对象。按照随机数字表法，将48只SD大鼠平均分为供鼠(n＝24)和受鼠(n＝24)。于SD大鼠处于动情期时将供鼠子宫组织缝合于受鼠腹部皮下，并于手术后3周取出异位病灶组织，于光学显微镜下观察异位病灶组织的形态学及组织病理学结果。

结果

48只SD大鼠均有规律的4个动情周期，分别为动情前期、动情期、动情后期、动情间期，各个周期分别为4～5 d。24只SD大鼠(受鼠)均存活，同种异体SD大鼠皮下移植法成模率为66.7%(16/24)，其中16只SD大鼠腹部肉眼可见球型凸起，触之呈囊实性，无活动性，切开皮肤可见透亮的球型囊状增生物，质地软，囊内有清亮液体聚积，平均体积为(34.5±19.8)mm³，异位病灶组织于光学显微镜低倍镜(×100)下可见异位病灶生长成一个腔样结构，于高倍镜下(×400)可见内膜上皮细胞、间质细胞及少量腺体形成，组织病理学表现与人EMS相似。

结论

通过皮下移植法建立的同种异体SD大鼠EMS模型的异位病灶组织的组织病理学改变与人EMS类似，可以作为EMS研究的动物模型。

关键词: 子宫内膜异位症, 模型，动物, 移植，同种, 大鼠

Objective

To explore the feasibility of the establishment of endometriosis (EMS) model in allogeneic rats by abdominal subcutaneous implantation.

Methods

Choose 48 healthy, unmated and in estrus (normal estrous cycle ≥ 2) female Sprague-Dawley (SD) rats as the research object. According to random number table, 48 SD rats were divided into donor rats (n=24) and recipient rats (n=24). When the SD rats in estrus, we sutured the donor rats uterine tissue to the abdominal subcutaneous of recipient rats, and removed the ectopic lesion tissue 3 weeks after surgery, then observe histopathologic findings under an optical microscope.

Results

All of those 48 SD rats had regular estrous cycles, there were 4 estrous cycles which were proestrus, estrus, metestrus, and diestrus, each period lasted 4-5 d. Twenty-four SD rats (recipient rats) were alive, the successful rate of allogeneic abdominal subcutaneous implantation of SD rats was 66.7%(16/24), in which 16 SD rats' abdomen could see dome projection, which was cystic and solid, non-active, and cut the skin open, we can see translucent dome cystic proliferator, texture soft, clear liquid accumulation in the capsule. The average volume was (34.5±19.8) mm³. Ectopic lesion tissue was observed through low magnification optical microscope (×100), and we could see ectopic lesions grew into a cavity-like structure. Through we could see endometrial epithelial cells, stromal cells and the formation of a small amount of glandular under high magnification optical microscope (×400). Tissue pathology was similar to human EMS.

Conclusions

The tissue pathology changes of ectopic endometrial in EMS model of allogeneic SD rats by subcutaneous transplantation were similar to human EMS, EMS SD rats model can be used in the studies on EMS.

Key words: Endometriosis, Models, animal, Transplantation, homologous, Rats

图1 SD大鼠动情周期中各阶段的阴道涂片表现（图1A：动情前期，多数为椭圆形的有核上皮细胞，偶见少量角化细胞；图1B：动情期，大量外形不规则的角化上皮细胞，呈“落叶状”堆积或相互连接成片，偶可见少量有核上皮细胞；图1C：动情后期，可见不规则角化上皮细胞、有核上皮细胞和白细胞均匀分布；图1D动情间期，绝大多数为白细胞及少量有核上皮细胞和黏液）（HE染色，×100）

Figure 1 Performance of each stage of the vaginal smear in the estrous cycle of SD rats (Figure 1A: proestrus, mostly oval nucleated epithelial cells, occasionally a small amount of keratinocytes; Figure 1B: estrus, a large number of irregular shape of keratinocyte,was "like leaves" stacked or interconnected to piece, even showing a small amount of nucleated epithelial cells; Figure 1C: metestrus, irregular keratinized epithelial cells, nucleated epithelial cells and white blood cells distributed uniformly; figure 1D:diestrus, vast majority of white blood cells and a small amount of nucleated epithelial cells and mucus) (HE staining, ×100)

图2 建模成功的SD大鼠异位病灶组织形态学表现。肉眼可见透亮的球型囊状增生物，质地软，囊内有清亮液体聚积

Figure 2 Morphological features of ectopic lesions.We can see translucent dome cystic proliferator, texture soft, clear liquid accumulation in the capsule

图3 正常子宫组织及异位病灶组织的组织病理学表现［图3A：正常SD大鼠子宫组织（HE染色，×100）；图3B：异位病灶组织，可见腔样结构（HE染色，×100）；图3C：异位病灶组织，可见内膜上皮细胞层、间质细胞及少量腺体（HE染色，×400）］

Figure 3 Histopathological performance of normal uterine tissue ande ctopic lesion tissue［Figure 3A: normal SD rat uterus (HE staining, ×100); Figure 3B: ectopic lesions, visible cavity-like structure (HE staining, ×100); Figure 3C: ectopic lesions, visible endometrial epithelial layer,stromal cells and a small amount of glandular (HE staining, ×400)］

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Establishment of endometriosis model in allogeneic Sprague-Dawley rats

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Establishment of endometriosis model in allogeneic Sprague-Dawley rats