探讨子痫前期患者胎盘中REDD1蛋白的表达情况。

选择2011年12月至2012年4月四川大学华西第二医院收治的确诊为子痫前期，年龄为(31. 4±1. 4)岁的单胎妊娠孕妇29例为研究对象。按照妊娠中子痫前期发生的时间，将其分为早发型子痫前期组(n= 15)和晚发型子痫前期组(n=14)。同时按入院顺序号随机选择孕期在本院产前检查，年龄为(32.0±2.8)岁的单胎妊娠健康孕妇20例纳入对照组（本研究遵循的程序符合四川大学华西第二医院人体试验委员会制定的伦理学标准，得到该委员会批准，分组征得受试对象知情同意，并与之签署临床研究知情同意书）。采用SP免疫组织化学染色技术，Western bloting杂交及实时荧光定量PCR检测受试者REDD1蛋白及其mRNA在胎盘组织中的表达，并采用相关统计学方法分析早发型子痫前期组、晚发型子痫前期组及对照组胎盘组织中REDD1蛋白表达差异。早发型子痫前期组及晚发型子痫前期组患者的一般临床资料（年龄、孕周及产次）分别与对照组比较，差异均无统计学意义(P>0.05)。

REDD1蛋白表达于胎盘滋养细胞的细胞质。早发型子痫前期组及晚发型子痫前期组胎盘组织中REDD1蛋白水平均较对照组显著增高，差异有统计学意义(P< 0.05)。早发型子痫前期组REDD1 mRNA表达水平较对照组明显增高，差异亦有统计学意义(P<0.05)；但晚发型子痫前期组与对照组比较，差异无统计学意义(P>0.05)。

REDD1蛋白可能在子痫前期发病机制中起重要作用。

To study the expression of REDD1 in women with pre-eclampsia (PE).

This study involved normal outcome pregnancies women with an average age of (32. 0 ± 2. 8)years old (n = 20, gestational age >37 weeks) and PE group with an average age of (31. 4 ± 1. 4) years old. According to the time of PE onset, pregnancies women with PE were divided into early-onset PE group (n=15,gestational age:24-33 weeks) and late-onset PE group(n= 14, gestational age:34-39 weeks). All of subjects were included from December 2011 to April 2012 in West China Second University Hospital, Sichuan University. The study protocol was approved by the Ethical Review Board of Investigation in Human Beings of West China Second University Hospital, Sichuan University. Informed consent was obtained from each participating patient. In experiments,REDD1 protein in the placenta was examined and located by SP immunohistochemistry, furthermore, REDD1 protein was ensured by Western blot. Additionally, REDD1 mRNA expression level was determined by relative quantitative real time fluorescence PCR. REDD1 protein and it's mRNA expression levels were compared between early-onset PE group,late-onset PE group and control group by advisable statistical methods. There had no significance difference in three groups among basic clinical parameters including age, gestational age and parity(P>0. 05).

REDD1 protein was predominantly observed in the cytoplasm of villous cytotrophoblast and trophoblast but rarely in fetoplacental vascular. Compared with control group: Expression of REDD1 protein more significantly elevated in both early-onset PE group and late-onset PE group (P<0. 05). Moreover, REDD1 mRNA level also had significantly increased in early-onset PE group than that in control group (P<0. 05); but there had no significance between REDD1 mRNA in late-onset PE group and that in control group.

REDD1 was expressed universally in placental villi and had functions in development of placenta. Increased REDD1 protein may play an important role in pathogenesis of preeclampsia.