不同剂量及疗程米非司酮治疗大鼠子宫内膜异位症的疗效

doi:10.3877/cma.j.issn.1673-5250.2012.06.005

中华妇幼临床医学杂志(电子版) ›› 2012, Vol. 08 ›› Issue (06) : 696 -699. doi: 10.3877/cma.j.issn.1673-5250.2012.06.005

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论著

不同剂量及疗程米非司酮治疗大鼠子宫内膜异位症的疗效

胡瑜凌¹, 乔林¹, 杨琳¹, 钟兰萍¹, 徐克惠¹^,^*^,^*(

)

^1. 610041　成都，四川大学华西第二医院妇产科

收稿日期:2012-08-01 修回日期:2012-11-01 出版日期:2012-12-01
通信作者: 徐克惠

Effect of Different Doses and Courses of Mifepristone in the Treatment of Rats' Endometriosis

Yu-ling HU¹, Lin QIAO¹, Lin YANG¹, Lan-pin ZHONG¹, Ke-hui XU^,¹

^1. Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China

Received:2012-08-01 Revised:2012-11-01 Published:2012-12-01
Corresponding author: Ke-hui XU
About author:
(Corresponding author: XU Ke-hui, Email: kehui X@yahoo.com)

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目的

探讨不同剂量、不同疗程的米非司酮(mifepristone)对减小子宫内膜异位症(EMs)病灶的疗效。

方法

建立EMs大鼠模型，将建模成功的79只大鼠随机分为高剂量组[米非司酮剂量为2.604 mg/(kg·d)]、低剂量组[米非司酮剂量为1.041 mg/(kg·d)]及对照组(1 mL花生油)，治疗期内死亡7只；将3组再按不同疗程分为20 d亚组，30 d亚组，40 d亚组，观察用药20 d，30 d及40 d时异位病灶体积的减小率，从而判断治疗的有效性。

结果

高剂量组及低剂量组分别与对照组疗效比较，异位病灶体积均显著减小，差异均有统计学意义(P<0.05)；而低剂量组与高剂量组的异位病灶体积减小率比较，差异无统计学意义(P＝0.662)，用药20 d，30 d及40 d时病灶减小率比较，差异亦无统计学意义(P＝0.559，0.145，0.057)。

结论

米非司酮可抑制EMs异位病灶的生长，低剂量组与高剂量组抑制趋势无显著差异，而且延长治疗周期，亦不能增强该药对异位内膜生长的抑制作用。

关键词: 子宫内膜异位症, 米非司酮, 大鼠

Objective

To investigate the effect of different dosages and different treatment methods of mifepristone in rat's endometriosis(EMs).

Methods

A total of 79 rats were selected to establishment of models of EMs, and were randomly divided into high-dose group[dosages of 2.604 mg/(kg·d)], low-dose group[dosages of 1.041 mg/(kg·d)], control group(placebo of 1 mL peanut oil)to determine the drugs effects of the ectopic lesions reduce by medication. There were 7 rats died during treatment period. According to different courses of treatment 20 days, 30 days and 40 days, 72 rats were divided into 9 sub-groups to determine the effectiveness of treatment.

Results

After treatment of mifepristone, ectopic lesions of EMs were significantly reduced than those before treatment in low-dose group and high-dose group (P<0.05); in low-dose group and high-dose group there were significance difference than those in control group (P<0.05); however, curative effects of mifepristone between low-dose group and high-dose group (P=0.662) or different courses of treatment 20 days, 30 days and 40 days subgroups had no statistically significant difference (P=0.559, 0.145, 0.057).

Conclusions

Mifepristone could inhibit the growth of EMs ectopic lesions. There was no statistically significant difference to treatment models of EMs rats by medication dosages of 2.604 mg/(kg·d), 1.041 mg/(kg·d) or different courses of treatment by medication 20 days, 30 days and 40 days subgroups.

Key words: endometriosis, mifepristone, rat

图1 大鼠EMs模型的透明囊泡(异位病灶)

Figure 1 Transparent vesicles model of EMs rat (Ectopic lesions)

图2 大鼠EMs模型的紫蓝色囊泡(异位病灶)

Figure 2 Violet blue vesicles model of EMs rat(Ectopic lesions)

图3 同时具有一层子宫内膜柱状腔上皮细胞和子宫内膜间质细胞(HE染色，10×10)

Figure 3 Endometrial columnar luminal epithelial cells and endometrial stromal cells(HE，10×10)

表1 3组及其亚组的异位病灶体积减小率比较(mm³，±s)

Table 1 Comparison of reduced rate of each group lesion volume (mm³，±s)

病灶减小率(%)	低剂量组	高剂量组	对照组	合计(只)
20 d亚组	52.03±45.33^ab	56.34±43.06^ab	－73.49±139.50	25
30 d亚组	36.58±35.21^ab	55.36±27.69^ab	－80.64±78.22	23
40 d亚组	74.35±18.67^ab	76.29±23.41^ab	－23.50±101.47	24
合计(只)	25	23	24	72

表1 3组及其亚组的异位病灶体积减小率比较(mm³，±s)

Table 1 Comparison of reduced rate of each group lesion volume (mm³，±s)

病灶减小率(%)	低剂量组	高剂量组	对照组	合计(只)
20 d亚组	52.03±45.33^ab	56.34±43.06^ab	－73.49±139.50	25
30 d亚组	36.58±35.21^ab	55.36±27.69^ab	－80.64±78.22	23
40 d亚组	74.35±18.67^ab	76.29±23.41^ab	－23.50±101.47	24
合计(只)	25	23	24	72

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