肺炎支原体感染儿童血外周血辅助性T细胞17型和白细胞介素－17变化及其临床意义

doi:10.3877/cma.j.issn.1673-5250.2012.02.012

中华妇幼临床医学杂志(电子版) ›› 2012, Vol. 08 ›› Issue (02) : 163 -166. doi: 10.3877/cma.j.issn.1673-5250.2012.02.012

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肺炎支原体感染儿童血外周血辅助性T细胞17型和白细胞介素－17变化及其临床意义

宋磊¹^,^*^,^*(

), 赵小冬¹, 杨小红¹, 孔炜¹, 周峰², 汪晓莺³

^1. 江苏南通　226001，江苏省南通市第一人民医院儿科
^2. 江苏省南通市第一人民医院检验科
^3. 南通大学免疫教研室

收稿日期:2011-11-02 修回日期:2012-02-29 出版日期:2012-04-01
通信作者: 宋磊

Changes of T Help Cell 17 and Interleukin-17 in Children With Mycoplasma Pneumoniae Infection and Its Clinical Significance

Lei SONG¹(), Xiao-dong ZHAO¹, Xiao-hong YANG¹, Wei KONG¹, Feng ZHOU², Xiao-ying WANG³

^1. Department of Pediatrics, Nantong First People's Hospital, 226001 Nantong, Jiangsu Province, China

Received:2011-11-02 Revised:2012-02-29 Published:2012-04-01
Corresponding author: Lei SONG
About author:
Corresponding author: SONG Lei, Email: songlei1972@126.com

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宋磊, 赵小冬, 杨小红, 孔炜, 周峰, 汪晓莺. 肺炎支原体感染儿童血外周血辅助性T细胞17型和白细胞介素－17变化及其临床意义[J]. 中华妇幼临床医学杂志(电子版), 2012, 08(02): 163-166.

Lei SONG, Xiao-dong ZHAO, Xiao-hong YANG, Wei KONG, Feng ZHOU, Xiao-ying WANG. Changes of T Help Cell 17 and Interleukin-17 in Children With Mycoplasma Pneumoniae Infection and Its Clinical Significance[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2012, 08(02): 163-166.

目的

探讨外周血辅助性T细胞(Th)17型和白细胞介素(IL)－17在肺炎支原体(MP)急性感染儿童体内变化及其临床意义。

方法

选择2009年6月至2010年12月在南通市第一人民医院儿科接受住院治疗并确诊为肺炎支原体肺炎(MPP)的32例患儿为研究对象，并纳入MPP组，平均年龄为(9.3±2.5)岁。将其进一步按照感染症状分为MPP亚组1(普通肺炎症状，n＝18)和MPP亚组2(肺炎合并肺外症状，n＝14)；选择同期于该院门诊行健康体检儿童纳入对照组(n＝26)(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准，得到该委员会批准，分组征得受试对象监护人的知情同意，并与之签署临床研究知情同意书)。MPP组与对照组、MPP亚组1与MPP亚组2受试者的年龄、性别等比较，差异无统计学意义(P>0.05)。常规分离外周血单核细胞(PBMC)，采用流式细胞术(FACS)检测各组外周血Th17；酶联免疫吸附法(ELISA)检测血清细胞因子IL－17水平。

结果

①MPP亚组1与MPP亚组2的外周血Th17频率均高于对照组[(2.05±0.65)% vs. (1.21±0.36)%, (2.98±0.72)% vs. (1.21±0.36)%；P<0.01]；MPP亚组2外周血Th17频率均高于MPP亚组1[(2.98±0.72)% vs. (2.05±0.65)%，P<0.01]。②MPP亚组1与MPP亚组2血清IL－17水平均高于对照组[(11.62±5.37) pg/mL vs. (6.45±1.62) pg/mL，(17.34±7.45) pg/mL vs. (6.45±1.62) pg/mL；P<0.01]；MPP亚组2血清IL－17水平高于MPP亚组1[(11.62±5.37) pg/mL vs. (17.34±7.45) pg/mL；P<0.01]。

结论

MP感染患儿体内Th17频率明显改变，且伴炎症性细胞因子IL－17水平升高，Th17频率异常在MP感染的发病机制中起重要作用。

关键词: 肺炎支原体, 辅助性T细胞17型, 白细胞介素－17

Objective

To study the change and clinical significances of helper T cell (Th) 17 and interleukin (IL)-17 in children with Mycoplasma pneumoniae (MP) infection.

Methods

A total of 32 children with MP pneumonia (MPP) were recruited in the Department of Pediatrics of Nantong People's Hospital from June 2009 to December 2010. They were selected into MPP group with the average age of (9.3±2.5) years old. They were further divided into MPP sub-group 1 (symptom of pneumonia, n=18) and MPP sub-group 2 (symptom with lesion of exo-lung, n=14). At the same time, a total of 26 health children who underwent physical examination were selected into control group. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Panzhihua Second People's Hospital. Informed consent was obtained from each participate. The peripheral blood mononuclear cells were isolated, then frequency of Th17 were determined by flow cytometry (FACS). Serum contents of IL-17 were detected by enzyme linked immunosorbent assay (ELISA).

Results

① The frequencies of Th17 cells of MP infection children in MPP sub-group 1 and MPP sub-group 2 were obviously higher than those in control group [(2.05±0.65)% vs. (1.21±0.36)%, (2.98±0.72)% vs. (1.21±0.36)%, P<0.01], the frequency of Th17 cells of MPP sub-group 2 was higher than that in MPP sub-group 1[(2.98±0.72)% vs. (2.05±0.65)%, P<0.01]. ② The serum levels of IL-17 in MP infection group were higher than those in control group [(11.62±5.37) pg/mL vs. (6.45±1.62) pg/mL, (17.34±7.45) pg/mL vs. (6.45±1.62) pg/mL, P<0.01], MPP sub-group 2 was higher than that in MPP sub-group 1[(11.62±5.37) pg/mL vs. (17.34±7.45) pg/mL, P<0.01].

Conclusions

Abnormal frequencies of Th17 and IL-17 levels play a key role in the pathogenic mechanism of MP infection in children.

Key words: Mpcoplasma pneumoniae, T help cell 17, interleukin-17

表1 MPP组与对照组Th17频率和IL－17水平比较(±s)

Table 1 Comparison of Th17 frequency and the level of IL－17 among MPP sub－group 1, MPP sub－group 2 and control group (±s)

组别		n	Th17频率(%)	IL－17水平(pg/mL)
MPP组		?	?	?
?	MPP亚组1	18	2.05±0.65^a	11.62±5.37^a
?	MPP亚组2	14	2.98±0.72^{a b}	17.34±7.45^{a b}
对照组		26	1.21±0.36	6.45±1.62

表1 MPP组与对照组Th17频率和IL－17水平比较(±s)

Table 1 Comparison of Th17 frequency and the level of IL－17 among MPP sub－group 1, MPP sub－group 2 and control group (±s)

组别		n	Th17频率(%)	IL－17水平(pg/mL)
MPP组		?	?	?
?	MPP亚组1	18	2.05±0.65^a	11.62±5.37^a
?	MPP亚组2	14	2.98±0.72^{a b}	17.34±7.45^{a b}
对照组		26	1.21±0.36	6.45±1.62

1	The Subspecialty Group of Respiratory Diseases. The Society of Pediatrics, Chinese Medical Association，The Editorial Board，Chinese Journal of Pediatrics. Guideline of management of community－acquired pneumonia [J]. Chin J Pediatr, 2007, 45(2):83-90.
2	Hou QN, Lai T, Qie MR. Effects of interleukin－17 on the expression of matric metalloproteinases－2，－9 in endometrial cell line HEC－1－B and metastasis of tumor cell [J/CD]. Chin J Obstet Gynecol Pediatr: Electron Ed, 2011, 7(2):105-108.
3	Blauvelt A. T－helper 17 cells in psoriatic plaques and additional genetic links between IL－23 and psoriasis [J]. J Invest Dermatol, 2008, 128(5):1064.
4	Fransson ME, Liljenfeldt LS, Fagius J, et al. The T－cell pool is anergized in patients with multiple sclerosis in remissio[J]．Immunology, 2009, 126(1):92-101.
5	Maloy KJ.The interleukin－23/interleukin－17 axis in intestinal inflammatio [J]. J Intern Med, 2008, 263(6):584-590.
6	Hu YM, Jiang ZF, ed. Zhu Fuu－tang practical pediatrics. 7th ed [M]. Beijing: People's Medical Publishing House, 2003, 1204-1205.
7	Li XL, Ying SH, Hua Z. Central nervous system infection of mycoplasma pneumoniae in 40 children [J]. Chin J Gen Pract, 2010, 8(7):861-862.
8	Wang H. Nervous system complications of mycoplasma pneumoniae infection[J]. J Appl Clin Pediatr, 2009, 24(10):726-729.
9	Wang JB, Deng HF, Lu MY, et al. Clinical analysis of 24 cases with Mycoplasma pneumoniae encephalitis [J]. Jiangsu Med, 2011, 37(9):1046-1048.
10	Vervloet LA, Marguet C, Camargos PA. Infection by Mycoplasma pneumoniae and its importance as an etiological agent in childhood community－acquired pneumonias[J]．Braz J Infect Dis, 2007, 11(5):507-514.
11	Jin-Won Bae, Hak-Jin Kim, Gregory Youngnam Chang, et al. Combined striatum, brain stem, and optic nerve involvement due to Mycoplasma pneumoniae in an ambulatory child [J]. Case Rep Neurol, 2011, 3(2):109-112.
12	Lee KY. Pediatric respiratory infections by Mycoplasma pneumonia [J]. Expert Rev Anti Infect Ther, 2008, 6(4):509-521.
13	Harrington LE, Hatton RD, Mangan PR, et al. Interleukin 17－producing CD₄^＋effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages[J]. Nat lmmunol, 2005, 6(11):1123-1132.
14	Park H, Li Z, Yang XO, et al. A distinct lineage of CD₄ T cells regulates tissue inflammation by producing interleukin 17[J]. Nat Immunol, 2005, 6(11):1133-1141.
15	van Beelen AJ, Teunissen MB, Kapsenberg ML, et al. Interleukin－17 in inflammatory skin disorders [J]. Curr Opin Allergy Clin Immunol, 2007, 7:374-381.
16	Afzali B, Lombardi G, Lechler RI, et al. The role of T helper 17(Th17) and regulatory T cells (Treg) in human organ transplantation and autoimmune disease [J]. Clin Exp Immunol, 2007, 148:32-46.
17	Oboki K, OhnoT, Saito H, et al. Th17 and allergy [J]. Allergol Int, 2008, 57(2):121-134.
18	Semik-Orzech A, Barczyk A, Pierzcha W. The role of interleukin 17A in inducing neutrophilic inflammation in the pulmonary tract[J]. Pol Merkur Lekarski, 2007, 22(129):163-168.

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