Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2016, Vol. 12 ›› Issue (02): 196 -200. doi: 10.3877/cma.j.issn.1673-5250.2016.02.012

Special Issue:

Original Article

Clinical analysis of throat swab culture in neonates

Tao Zhong1, Juan Chen1, Lei Yang1()   

  1. 1. Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2015-11-18 Revised:2016-02-29 Published:2016-04-01
  • Corresponding author: Lei Yang
  • About author:
    Corresponding author: Yang Lei, Email:
Objective

To study the clinical significance of bacterial colonization in neonatal infection through intergrating the throat swab culture and other clinical data in admitted neonates within 24 hours.

Methods

The clinical data of 934 newborns (527 boys and 407 girls) who were admitted to Sichuan Provincial People's Hospital (East Region) from January 2011 to June 2014 were analyzed retrospectively. Inclusion criteria of this study were hospitalized neonates with complete clinical data, and the exclusion criteria were neonates without complete clinical data and automatic discharge. All of them were taken throat swab culture once during 24 hours after admission. The bacterial colonization, relationship between premature rupture of membranes (PROM) and neonatal infection, relationship between preterm and full-term neonates and infection were detected. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Sichuan Provincial People's Hospital (East Region).

Results

① Four hundreds and eight-seven samples (52.1%) showed normal mixed bacteria, 259 samples (27.7%) were pathogenic bacteria, another 188 samples (20.1%) were negative in 934 throat swab culture results from 2011 to 2014. Top 5 pathogenic bacteria were Staphylococcus epidermidis (43 strains, 16.6%), Hemophilus parainfluenzae (26 strains, 10.0%), Hemophilus influenzae (21 strains, 10.0%), Pneumonia kleiber (21 strains, 8.1%) and Escherichia coli (19 strains, 7.3%) in 4 years. ②There was statistical significance between respiratory tract infection rates in neonates with PROM (96.7%) and non-PROM cases (85.6%) (χ2=5.692, P=0.013). Throat swab culture positive in neonates with PROM>24 hours (70.0%) was higher than that in non-PROM term (50.7%), which had statistical significance (χ2=6.685, P=0.013). ③ Ninety-two cases(55.8%) who received throat swab culture showed positive in premature infants, and 654 cases (85.0%) showed positive in full-term infants. The positive rate of throat swab culture and positive rate of throat swab culture combined with infection of full-term neonates were higher than those of preterm neonates (85.0% vs 55.8%, 53.5% vs 37.0%), which had significant differences (χ2=72.453, 8.855; P<0.05). Besides, there was statistical significance between premature and full-term infants in throat swab culture comparing mixed and single bacteria test (χ2=16.975, 55.107; P<0.001).

Conclusions

Throat swab culture can be useful in identifying bacterial colonization during 24 hours after admission, especially for those with PROM.

表1 2011-2014年各年份咽拭子培养结果情况[份(%)]
1
刘健慧,王丹华.新生儿重症监护病房早产儿细菌定植的临床研究[J].中国实用儿科杂志,2006,21(1):41-44.
2
王羽,张宗久,赵明钢,等.全国临床检验操作规程[M].南京:东南大学出版社,2006:40-42.
3
Clinical and Laboratory Standards Institute. 抗菌性药物敏感性实验执行标准.孙长贵,杨启文,杨继勇,译[S]. 北京:中华检验医学杂志CLSI临床检验标准编译小组,2013.
4
张丽红,柴建华,常洪美.某院新生儿重症监护室定植菌筛查及临床意义[J].中国感染控制杂志,2013,12(2):120-122.
5
蒋人贵,冯伊利,李成军,等.胎膜早破导致新生儿感染的常见病原菌分布[J].中华医院感染学杂志,2013,23(5):1094-1096.
6
张海金,赵玉,程丽萍.新生儿感染病原菌检测及耐药特征研究[J].中国妇幼保健,2015,30(21):3608-3610.
7
黄小翠,熊彬鉴,崔亚利.新生儿细菌性肺炎感染监测及其药敏分析[C]//中国中西医结合学会.第一次全国中西医结合检验医学学术会议暨中国中西医结合学会检验医学专业委员会成立大会论文汇编.北京,2014.
8
Lin YC, Lauderdale TL, Lin HM, et al. An outbreak of methicillin-resistant Staphylococcus aureus infection in patients of a pediatric intensive care unit and high carriage rate among health care workers[J]. J Microbiol Immunol Infect, 2007, 40(4): 325-334.
9
武书丽.胎膜早破羊水与新生儿咽拭子细菌培养分析[J].中国优生与遗传杂志,2011,31(1):79-80.
10
王迎,邓颖.新生儿败血症患儿母亲围生期感染因素及其临床症状和体征分析[J].中国全科医学,2013,16(27):3179-3182.
11
Hallab JC, Leach ST, Zhang L, et al. Molecular characterization of bacterial colonization in the preterm and term infant's intestine[J]. Indian J Pediatr, 2013, 80(1): 1-5.
12
Cilieborg MS, Boye M, Sangild PT. Bacterial colonization and gut development in preterm neonates[J]. Early Hum Dev, 2012, 88 (Suppl 1): S41-S49.
13
Hokama T, Yara A, Hirayama K, et al. Isolation of respiratory bacterial pathogens from the throats of healthy infants fed by different methods[J]. J Trop Pediatr, 1999, 45(3): 173-176.
14
徐焱,王丹华,徐英春.新生儿重症监护病房中部分定植细菌定植特点及变化规律[J].临床儿科杂志,2008,26(3):209-212.
15
侯安存,刘玉华,辛德莉,等.健康儿童鼻咽部常见致病微生物携带状况及临床意义[J].中华儿科杂志,2002,40(1):48-52.
16
黄懿洁.呼吸道细菌定植对毛细支气管炎患儿预后与转归的影响[D].重庆:重庆医科大学,2010.
17
常璠.呼吸道感染患儿流感嗜血杆菌的生物分型与耐药性研究[J].国际检验医学杂志,2013,34(21):2922-2923.
18
谢成彬,王频佳,吴雨露,等.儿童呼吸道流感嗜血杆菌分离株耐药性与β-内酰胺酶基因研究[J].中国微生态学杂志,2013,25(5):561-565.
19
Zeng LN, Zhang LL, Shi J, et al. The primary microbial pathogens associated with premature rupture of the membranes in China: a systematic review[J]. Taiwan J Obstet Gynecol, 2014, 53(4): 443-451.
20
Mellata M. Human and avian extraintestinal pathogenic Escherichia coli: infections, zoonotic risks, and antibiotic resistance trends[J]. Foodborne Pathog Dis, 2013, 10(11): 916-932.
21
徐焱.NICU院内感染的流行病学调查和新生儿细菌定植的特点及变化规律[D].北京:中国协和医科大学,2007.
22
范含笑.3 000例足月新生儿感染性疾病临床资料分析[D].合肥:安徽医科大学,2014.
23
Cairns S, Thomas JG, Hooper SJ, et al. Molecular analysis of microbial communities in endotracheal tube biofilms[J]. PLoS One, 2011, 6(3): e14759.
24
Reimund JM, Arondel Y, Finck G, et al. Catheter-related infection in patients on home parenteral nutrition: results of a prospective survey[J]. Clin Nutr, 2002, 21(1): 33-38.
[1] Ling Wei, Hui Li, Yi Chen. Current research status on colonization or infection and mother-to-child transmission of extended-spectrum β-lactamase-producing Enterobacteriaceae in pregnant and post-partum women[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 517-521.
[2] Quan Wei, Shen Zhang, Huijia Chen, Heng Zou, Lina Hu. Current research progress on correlation between female reproductive tract microbiota and assisted reproductive technology[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(02): 151-155.
[3] Aimei Su, Yongtao Wei, Dongping Wang. Resistance and combined drug sensitivity test of carbapenem-resistant Enterobacteriaceae[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2023, 17(05): 333-340.
[4] Zengtao Wang, Xianbo Wang, Yu Cao, Yu Hao, Junyan Han, Hui Zeng. Dimensionality reduction and clustering method of flow cytometric data for measuring T cell subpopulations in patients with spontaneous bacterial peritonitis[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2023, 17(02): 92-101.
[5] Yixue Li, Ting Pan, Huancai Lin, Yan Zhou. Research progress of plant-derived natural products against oral bacterial infectious disease[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2023, 17(03): 188-196.
[6] Xinyi Deng, Zhenyu Zeng, Xiaolan Li. Research progress on bacterial quorum sensing signal on host immune regulation mechanism[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2023, 17(02): 140-147.
[7] Xiaodan Wang, Yuan Wang, Xiangyu Cui, Xiaolei Ren. Analysis of pathogenic bacteria resistance of drug and high risk factors of death in urogenic sepsis after endoscopic surgery for upper urinary tract stones[J]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(06): 611-615.
[8] Jinlin Xu, Zheng Chen. The impact of clinical monitoring of antimicrobial drugs on prophylactic medication and infection in inguinal hernia repair surgery[J]. Chinese Journal of Hernia and Abdominal Wall Surgery(Electronic Edition), 2023, 17(06): 720-723.
[9] Haoyue Xu, Kui Dong, Xuan Ji, Zhiming Kang, Junhong Li. Advance on ocular bacterial species influence on ocular health status in infants and children[J]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2023, 13(02): 114-118.
[10] Xin Jiang, Chun Pan, Wenhui Huang, Guifen Gan. A study on active CRE screening and CRE infection in anal swabs in Intensive Care Units in Qinghai area[J]. Chinese Journal of Critical Care & Intensive Care Medicine(Electronic Edition), 2023, 09(02): 178-184.
[11] Qiuqiong Li, Jing Xue, Min Wang, Fen Chen, Meifang Xiao. Diagnostic value of serum and cerebrospinal fluid levels of NSE, SIL-2R, and TNF-α in children with viral meningitis and bacterial meningitis[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(03): 303-307.
[12] Xiaosu Wang, Zheng Dai, Jiajia Zhu, Qichao Li, Litao Zhang. Comparative study on the detection ability of BacT/ALERT two blood culture systems and eight blood culture bottles for simulated bacteremia specimens[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(04): 207-213.
[13] Pingjuan Liu, Kecheng Luo, Jiayin Wu, Kang Liao, Wenwen Hu, Yili Chen. Epidemiological study on intestinal active surveillance of carbapenem-resistant Enterobacteriaceae in neurology intensive care unit[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(04): 235-240.
[14] Ruifu Yang, Yanbin Zhou. Research progress on the correlation of major facilitator superfamily membrane transporters and bacterial biofilm formation[J]. Chinese Journal of Diagnostics(Electronic Edition), 2023, 11(02): 77-81.
[15] Yongbing Sun, Jing Zhou, Dongpeng Wen, Junya He, Xinbei Lin, Qi Qiao, Zhonglin Li, Cheng Zhang, Zhikai Wang, Jiancheng Zhang, Xiaoling Wu, Zhi Zou, Yong Wang, Yongli Li. Research progress of brain-enteric-bacterial axis mechanism in weight-loss metabolic surgery[J]. Chinese Journal of Obesity and Metabolic Diseases(Electronic Edition), 2023, 09(01): 43-49.
Viewed
Full text


Abstract