Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2015, Vol. 11 ›› Issue (4): 492 -496. doi: 10.3877/cma.j.issn.1673-5250.2015.04.012

Special Issue:

Original Article

Expression and significance of interleukin-17 in cervical cancer

Shiyu Chen, Yi Quan, Xi Zeng, Mingrong Qie*()   

  1. Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2014-12-10 Revised:2015-06-01 Published:2015-08-01
  • Corresponding author: Mingrong Qie
Objective

To evaluate the expression of interleukin (IL)-17 in cervical cancer and analyze the association between the expression of IL-17 and its different clinical characteristics.

Methods

From January 2011 to January 2013, a total of 61 samples of cervical cancer (cervical squamous cell carcinoma) tissuse were enrolled into study group. At the same time, another 9 case of samples from benign uterine disease (uterine fibroids, adenomyosis) were enrolled into control group. Expression levels of IL-17 in the 9 cases paraffin sections of normal cervical tissues and 61 cases paraffin sections of cervical cancer were detected by streptavidin-biotin complex immunohistochemistry (SABC-IHC). The relationship between the IL-17 expression and its different clinical characteristics were analyzed by statistical method.

Results

①IL-17 expressed mainly in the cytoplasm of cervical cancer cells. ②The positive rate of IL-17 expression in study group was 54.1%. The difference of positive rate of IL-17 expression in two groups was significant (χ2=7.17, P<0.01). ③The positive rate of IL-17 expression was significantly higher in patients with peritumor intravascular cancer emboli (66.7%) than those without peritumor intravascular cancer emboli (39.3%), and the difference was significantly different (χ2=4.57, P=0.05); the positive rate of IL-17 expression in patients with International Federation of Gynecology and Obstetrics (FIGO) stage Ⅱ was significantly higher than those with FIGO stage Ⅰ, and the difference was significantly different (χ2=5.59, P=0.05).

Conclusions

The expression of IL-17 was associated with the development, infiltration and metastasis of cervical cancer. IL-17 may be an important factor for clinical diagnosis and prognosis of cervical cancer.

表1 两组IL-17表达情况比较[例数(%)]
表2 研究组患者IL-17表达与宫颈癌不同临床病理特征间的关系[例数(%)]
图1 两组标本免疫组织化学染色结果比较(图1A:IL-17在宫颈癌组织中表达呈阴性,SABC-IHC染色,×200;图1B:IL-17在宫颈癌组织中表达呈阳性,SABC-IHC染色,×200;图1C:IL-17在正常宫颈组织中表达呈阴性,SABC-IHC染色,×200;图1D:IL-17在宫颈癌组织中表达呈阴性,SABC-IHC染色,×400;图1E:IL-17在宫颈癌组织表达呈阳性,SABC-IHC染色,×400;图1F:IL-17在正常宫颈组织中表达呈阴性,SABC-IHC染色,×400)
[10]
Lee SY, Yoon BY, Kim JI, et al. Interleukin-17 increases the expression of Toll-like receptor 3 via the STAT3 pathway in rheumatoid arthritis fibroblast-like synoviocytes[J]. Immunology, 2014, 141(3): 353–361.
[11]
Kostic M, Dzopalic T, Zivanovic S, et al. IL-17 and glutamate excitotoxicity in the pathogenesis of multiple sclerosis[J]. Scand J Immunol, 2014, 79(3): 181–186.
[12]
Lee Y. The role of interleukin-17 in bone metabolism and inflammatory skeletal diseases[J]. BMB Rep, 2013, 46(10): 479–483.
[13]
Numasaki M, Fukushi J, Ono M, et al. Interleukin-17 promotes angiogenesis and tumor growth[J]. Blood, 2003, 101(7): 2620–2627.
[14]
Tartour E, Fossiez F, Joyeux I, et al. Interleukin 17, a T-cell-derived cytokine, promotes tumorigenicity of human cervical tumors in nude mice[J]. Cancer Res, 1999, 59(15): 3698–3704.
[15]
Benchetrit F, Ciree A, Vives V, et al. Interleukin-17 inhibits tumor cell growth by means of a T-cell-dependent mechanism[J]. Blood, 2002, 99(6): 2114–2121.
[16]
Martin-Orozco N, Chung Y, Chang SH, et al. Th17 cells promote pancreatic inflammation but only induce diabetes efficiently in lymphopenic hosts after conversion into Th1 cells[J]. Eur J Immunol, 2009, 39(1): 216–224.
[17]
Steiner GE, Newman ME, Paikl D, et al. Expression and function of pro-inflammatory interleukin IL-17 and IL-17 receptor in normal, benign hyperplastic, and malignant prostate[J]. Prostate, 2003, 56(3): 171–182.
[18]
Sfanos KS, Bruno TC, Maris CH, et al. Phenotypic analysis of prostate-infiltrating lymphocytes reveals Th17 and Treg skewing[J]. Clin Cancer Res, 2008, 14(11): 3254–3261.
[19]
Zhang B, Rong G, Wei H, et al. The prevalence of Th17 cells in patients with gastric cancer[J]. Biochem Biophys Res Commun, 2008, 374(3): 533–537.
[20]
Horlock C, Stott B, Dyson PJ, et al. The effects of trastuzumab on the CD4CD25FoxP3 and CD4IL17A T-cell axis in patients with breast cancer[J]. Br J Cancer, 2009, 100(7): 1061–1067.
[21]
Yang L, Qi Y, Hu J, et al. Expression of Th17 cells in breast cancer tissue and its association with clinical parameters[J]. Cell Biochem Biophys, 2012, 62(1): 153–159.
[22]
Kryczek I, Banerjee M, Cheng P, et al. Phenotype, distribution, generation, and functional and clinical relevance of Th17 cells in the human tumor environments[J]. Blood, 2009, 114(6): 1141–1149.
[23]
Kryczek I, Wei S, Zou L, et al. Cutting edge: Th17 and regulatory T cell dynamics and the regulation by IL-2 in the tumor microenvironment[J]. J Immunol, 2007, 178(11): 6730–6733.
[24]
Su X, Ye J, Hsueh EC, et al. Tumor microenvironments direct the recruitment and expansion of human Th17 cells[J]. J Immunol, 2010, 184(3): 1630–1641.
[25]
Chen X, Wan J, Liu J, et al. Increased IL-17-producing cells correlate with poor survival and lymphangiogenesis in NSCLC patients[J]. Lung Cancer, 2010, 69(3): 348–354.
[1]
Bettelli E, Korn T, Oukka M, et al. Induction and effector functions of Th17 cells[J]. Nature, 2008, 453(7198): 1051–1057.
[2]
Lan C, Huang X, Lin S, et al. High density of IL-17-producing cells is associated with improved prognosis for advanced epithelial ovarian cancer[J]. Cell Tissue Res, 2013, 352(2): 351–359.
[3]
Li Q, Li Q, Chen J, et al. Prevalence of Th17 and Treg cells in gastric cancer patients and its correlation with clinical parameters[J]. Oncol Rep, 2013, 30(3): 1215–1222.
[4]
Chang YH, Yu CW, Lai LC, et al. Up-regulation of interleukin-17 expression by human papillomavirus type 16 E6 in nonsmall cell lung cancer[J]. Cancer, 2010, 116(20): 4800–4809.
[5]
Zhang JP, Yan J, Xu J, et al. Increased intratumoral IL-17-producing cells correlate with poor survival in hepatocellular carcinoma patients[J]. J Hepatol, 2009, 50(5): 980–989.
[6]
Gaffen SL. Biology of recently discovered cytokines: interleukin-17:a unique inflammatory cytokine with roles in bone biology and arthritis[J]. Arthritis Res Ther, 2004, 6(6): 240–247.
[7]
Huang SH, Frydas S, Kempuraj D, et al. Interleukin-17 and the interleukin-17 family member network[J]. Allergy Asthma Proc, 2004, 25(1): 17–21.
[8]
Kolls JK, Linden A. Interleukin-17 family members and inflammation[J]. Immunity 2004, 21(4): 467–476.
[9]
Song X, Qian Y. IL-17 family cytokines mediated signaling in the pathogenesis of inflammatory diseases[J]. Cell Signal, 2013, 25(12): 2335–2347.
[26]
Maruyama T, Kono K, Mizukami Y, et al. Distribution of Th17 cells and FoxP3(+) regulatory T cells in tumor-infiltrating lymphocytes, tumor-draining lymph nodes and peripheral blood lymphocytes in patients with gastric cancer[J]. Cancer Sci, 2010, 101(9): 1947–1954.
[27]
Kesselring R, Thiel A, Pries R, et al. Human Th17 cells can be induced through head and neck cancer and have a functional impact on HNSCC development[J]. Br J Cancer, 2010, 103(8): 1245–1254.
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