Analysis of Transforming Growth Factor-β1,Angiotensin Ⅱ and Vascular Endothelial Growth Factor Expression Levels in Women With Hypertensive Disorder Complicating Pregnancy

doi:10.3877/cma.j.issn.1673-5250.2014.06.017

Objective

To compare the expression level differences of transforming growth factor (TGF)-β1, angiotensin (Ang) Ⅱ and vascular endothelial growth factor (VEGF) in maternal blood, umbilical cord blood and the placenta surface in women with hypertensive disorder complicating pregnancy and normal pregnant women, and provide the clinical evidence for exploring the mechanisms of hypertensive disorder complicating pregnancy.

Methods

A total of 50 pregnant women with hypertensive disorder complicating pregnancy without other complications who delivered in Chengdu Women's & Children's Central Hospital from January 2009 to December 2012 were bringing into study group and 50 normal pregnant women who delivered at the same hospital and same time were bringing into control group. Placenta tissue of maternal and fetal surface,maternal blood and umbilical cord blood during childbirth were obtained. The expression levels of TGF-β1, Ang Ⅱ and VEGF were detected by enzyme linked immunosorbent assay (ELISA) and those expression levels were analyzed statistically. There were no statistically significant differences between two groups in maternal age,gestational age and body weight(P> 0.05). This study followed the Ethics Standards of Human Trial Committee of Chengdu Women's & Children's Central Hospital, and was approved by the Human Trial Committee. All subjects signed the informed consent.

Results

①The expression levels of TGF-β1 in maternal blood, umbilical cord blood, placenta maternal surface and fetal surface in hypertensive disorder complicating pregnancy group were significantly lower than those in control group while the expression levels of Ang Ⅱ were significantly higher,and the differences were statistically significant (P<0.05).②The expression levels of VEGF in umbilical cord blood, placenta maternal surface and fetal surface in hypertensive disorder complicating pregnancy group were significantly lower than those in control group, and the differences were statistically significant (P<0.05) ; But, there were no statistically significant differences between two groups of VEGF expression level in maternal blood (t = 0.639, P>0.05).

Conclusions

The change of expression levels of TGF-β1,Ang Ⅱ and VEGF plays an important role in occurrence and progress of hypertensive disorder complicating pregnancy.

Key words: Hypertension, pregnancy-induced, Transforming growth factor beta, Angiotensin Ⅱ, Vascular endothelial growth factor

表1 两组TGF-β1表达水平比较（ng/mL，±s）

Table 1 Comparison of TGF-β1 expression levels between two groups(ng/mL, ±s)

组别	例数	胎盘母面组织	胎盘子面组织	母亲静脉血	新生儿脐静脉血
妊娠期高血压疾病组	50	5.6±1.4	5.2±1.6	6. 0±1.8	6. 6±1.6
对照组	50	6.4±1.8	6. 0±1.6	6. 8±1.9	6.9±1.8
t值		2.707	2.525	2.032	2.099
P值		0.008	0.013	0.045	0.038

表1 两组TGF-β1表达水平比较（ng/mL，±s）

Table 1 Comparison of TGF-β1 expression levels between two groups(ng/mL, ±s)

组别	例数	胎盘母面组织	胎盘子面组织	母亲静脉血	新生儿脐静脉血
妊娠期高血压疾病组	50	5.6±1.4	5.2±1.6	6. 0±1.8	6. 6±1.6
对照组	50	6.4±1.8	6. 0±1.6	6. 8±1.9	6.9±1.8
t值		2.707	2.525	2.032	2.099
P值		0.008	0.013	0.045	0.038

表2 两组AngⅡ表达水平比较（ng/mL，±s）

Table 2 Comparison of Ang Ⅱ expression levels between two groups(ng/mL,±s)

组别	例数	胎盘母面组织	胎盘子面组织	母亲静脉血	新生儿脐静脉血
妊娠期高血压疾病组	50	9.4±3.7	14.9±4.5	44.5±9.9	57.5±13.8
对照组	50	8.0±3.3	12.7±3.6	39.8±9.1	52.1±13.5
t值		2.014	2.651	2.495	2.004
P值		0.047	0.009	0.014	0.048

表2 两组AngⅡ表达水平比较（ng/mL，±s）

Table 2 Comparison of Ang Ⅱ expression levels between two groups(ng/mL,±s)

组别	例数	胎盘母面组织	胎盘子面组织	母亲静脉血	新生儿脐静脉血
妊娠期高血压疾病组	50	9.4±3.7	14.9±4.5	44.5±9.9	57.5±13.8
对照组	50	8.0±3.3	12.7±3.6	39.8±9.1	52.1±13.5
t值		2.014	2.651	2.495	2.004
P值		0.047	0.009	0.014	0.048

图1 妊娠期高血压疾病及正常孕妇胎盘母面VEGF表达水平比较（IHC染色，DAB显色，× 100）

Figure 1 Comparison of VEGF expression levels on maternal surface between women with hypertensive disorder complicating pregnancy and normal pregnant women(IHC staining,DAB coloration,× 100)

图2 妊娠期高血压疾病及正常孕妇胎盘子面VEGF表达水平比较（IHC染色，DAB显色，×100）

Figure 2 Comparison of VEGF expression levels on fetal surface of placenta between women with hypertensive disorder complicating pregnancy and normal pregnant women（IHC staining,DAB coloration,× 100）

表3 两组VEGF表达水平比较（ng/mL，±s）

Table 3 Comparison of VEGF expression levels between two groups(ng/mL, ±s)

组别	例数	胎盘母面组织	胎盘子面组织	母亲静脉血	新生儿脐静脉血
妊娠期高血压疾病组	50	19.7±8.7	19.6±6.8	20.5±7.9	18.2±5.3
对照组	50	23.7±11.1	26.1±9.6	21.7±8.1	23.6±8.7
t值		2.009	2.906	0.639	3.454
P值		0.047	0.000	0.524	0.001

表3 两组VEGF表达水平比较（ng/mL，±s）

Table 3 Comparison of VEGF expression levels between two groups(ng/mL, ±s)

组别	例数	胎盘母面组织	胎盘子面组织	母亲静脉血	新生儿脐静脉血
妊娠期高血压疾病组	50	19.7±8.7	19.6±6.8	20.5±7.9	18.2±5.3
对照组	50	23.7±11.1	26.1±9.6	21.7±8.1	23.6±8.7
t值		2.009	2.906	0.639	3.454
P值		0.047	0.000	0.524	0.001

[1]	蔡秋荣.妊娠期高血压疾病临床特点分析[J].中国妇幼保健，2012, 27(25):3910–3911.
[2]	Roelen BA, Goumans MJ, Zwijsen A, et al. Identification of two distinct functions for TGF-beta in early mouse development [J]. Differentiation, 1998, 64 (1) : 19–31.
[3]	Nielsen AH, Schauser KH, Poulsen K. Current topic: the uteroplacental renin-angiotensin system [J]. Placenta, 2000, 21 (5-6)：468–477.
[4]	李妹燕.血管内皮生长因子与妊娠及流产的研究进展[J].右江民族医学院学报，2008，30(5) :842–843.
[5]	Yano K, Brown LF, Detmar M. Control of hair growth and follicle size by VEGF-mediated angiogenesis[J]. J Clin Invest, 2001, 107 (4)：409–417.
[6]	李芹，仇姝，王建军.VEGF蛋白在子痫前期胎盘中的表达及意义[J].河北医学，2010，16(10): 1157–1160.
[7]	耿丽红，蔡霞，王冬梅，等.妊高征胎盘中VEGF的表达和滋养细胞分泌功能的关系[J].新疆医科大学学报，2003，26(5)：467–469.
[8]	Svedas E, Islam KB, Nisell H, et al. Vascular endothelial growth factor induced functional and morphologic signs of endothelial dysfunction in isolated arteries from normal pregnant women[J]. Am J Obstet Gynecol, 2003, 188(1) : 168–176.
[9]	Jaeger LA, Spiegel AK, Ing NH, et al. Functional effects of transforming growth factor beta on adhesive properties of porcine trophectoderm[J]. Endocrinology, 2005, 146(9) : 3933–3942.
[10]	Li RH, Zhuang LZ. The effects of growth factors on human normal placental cytotrophoblast cell proliferation[J]. Hum Reprod，1997，12(4)：830–834.
[11]	Cox BE, Word RA, Rosenfeld CR. Angiotensin Ⅱ receptor characteristics and subtype expression in uterine arteries and myometrium during pregnancy[J]. J Clin Endocrinol Metab, 1996, 81(1)：49–58.
[12]	McMullen JR, Gibson KJ, Lumbers ER, et al. Interactions between AT1 and AT2 receptors in uterine arteries from pregnant ewes [J]. Eur J Pharmacol, 1999, 378(2) : 195–202.
[13]	Poston L. The control of blood flow to the placenta [J]. Exp Physiol, 1997, 82(2):377–387.
[14]	Phillips MI, Kagiyama S. Angiotensin Ⅱ as a pro-inflammatory mediator[J]. Curr Opin Investig Drugs，2002，3(4) : 569–577.

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