Effect and Mechanism of Neonate-mother Separation on the Visceral Sensitivity of Pain in Adult Rats

doi:10.3877/cma.j.issn.1673-5250.2014.01.007

Objective

To explore the effect and it's mechanism of neonate-mother separation (NMS) stimulation in neonatal period on the visceral sensitivity of pain in adult rats.

Methods

Establish NMS animal models. Take the pregnancy Wistar as the target, after production, then put 12 neonatal rats and a lactating female rat per cage. The subjects were divided into 2 groups: neonatal-mother separation (NMS) group (n=12, 3 hours'separation from maternal a day from 2 days to 21 days old) and control group (N) (n=12, no separation) . On the 2 months old, balloon distension of colorectum was conducted in these rats in adulthood. Rats at different pressures by colorectal distension (CRD) after stimulation of abdominal withdrawal reflex (AWR) scores for visceral pain sensitivity were evaluated, and then take distal colon to detect the expression of 5-HT and it's receptor by immunohistochemistry method.

Results

The AWR scores increased gradually with the rising of the CRD pressure in each group; The AWR scores were obviously higher in NMS group (P<0.05) . After colonic distension, the expression of serotonin and it's receptor significantly increased in colon of NMS group compared with the control group.

Conclusions

NMS could decrease pain threshold caused by colorectal distension in adult rats, and result in chronic visceral hyperalgesia. 5-HT and it's receptor system could play an important role in the neurochemical response to visceral noxious stimulation in adulthood.

Key words: Maternal deprivation, Irritable bowel syndrome, Visceral pain, Serotonin

表1 两组大鼠CRD后AWR评分比较（分，±s）

Table 1 Comparison of the AWR between two groups after CRD（分，±s）

组别	n	气囊注射不同气量(mL)
组别	n	1	3	5	7
NMS组	12	2.56 ± 0.34	3.72±0.25	4.25±0.28	4.18±0.31
N组	12	0.35 ± 0.21	0.83±0.36	1.67±0.17	2.54±0.15
t		6.34	7.26	6.85	5.48
P		0.000	0.000	0.000	0.000

表1 两组大鼠CRD后AWR评分比较（分，±s）

Table 1 Comparison of the AWR between two groups after CRD（分，±s）

组别	n	气囊注射不同气量(mL)
组别	n	1	3	5	7
NMS组	12	2.56 ± 0.34	3.72±0.25	4.25±0.28	4.18±0.31
N组	12	0.35 ± 0.21	0.83±0.36	1.67±0.17	2.54±0.15
t		6.34	7.26	6.85	5.48
P		0.000	0.000	0.000	0.000

图1 新生期母婴分离后对大鼠结肠组织5-HT表达的影响（SP免疫组化染色，×400）

Figure 1 The effect of NMS on 5-HT expression of distal colon in rat （SP stain，× 400）

图2 新生期母婴分离后对大鼠结肠组织5-HTR表达的影响（SP免疫组化染色，× 400）

Figure 2 The effect of NMS on 5-HTR expression of distal colon in rat （SP stain，×400）

表2 两组大鼠结肠5-HT及5-HTR表达比较（±s）

Table 2 Comparison of the expression of 5-HT and 5-HTR in colon between two groups（±s）

组别	n	大鼠结肠5-HT及5-HTR平均IOD值
组别	n	5-HT	5-HTR
NMS组	12	213.17±78.46	189.70±45.06
N组	12	115.72±69.12	102.88±45.39
t		8.12	3.25
P		0.000	0.002

表2 两组大鼠结肠5-HT及5-HTR表达比较（±s）

Table 2 Comparison of the expression of 5-HT and 5-HTR in colon between two groups（±s）

组别	n	大鼠结肠5-HT及5-HTR平均IOD值
组别	n	5-HT	5-HTR
NMS组	12	213.17±78.46	189.70±45.06
N组	12	115.72±69.12	102.88±45.39
t		8.12	3.25
P		0.000	0.002

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