Effects of Ginaton and Curcumin on Matrix Metalloproteinase-9 Expression Changes of Newborn Rats With Hypoxic-Ischemic Encephalopathy

doi:10.3877/cma.j.issn.1673-5250.2013.05.015

Objective

To explore the matrix metalloproteinase (MMP)-9 expression changes of newborn rats with hypoxic-ischemic encephalopathy (HIE) after ginaton and curcumin treatment.

Methods

Ninety newborn 7 days Sprague-Dawley rats were randomly divided into five groups, sham operation control group (n=18), HIE group(n=18), HIE+ ginaton intervention group (ginaton group, n=18), HIE+ curcumin intervention group(curcumin group, n=18), HIE+ ginaton+ curcumin intervention group(comprehensive group, n=18). Those five groups were further divided into three sub-group after HIE, 6 h sub-group, 24 h sub-group, 48 h sub-group. The change tendency of MMP-9 after treating by ginaton and curcumin were observed.

Results

Compared with sham group and HIE group, the 6 h, 24 h, and 48 h sub-group of immunoreactive cells in the average gray value among ginaton group, curcumin group comprehensive group had statistically significant difference (P<0.01), and there still had statistically significant difference between ginaton group and comprehensive group, curcumin group and comprehensive group (P<0.01).

Conclusion

The effects of ginaton combined with curcumin had obvious improvement on the nervous system functions recovery after HIE.

Key words: ginaton, curcumin, hypoxic-ischemic encephalopathy, newborn rats, matrix metalloproteinase-9

表1 各组MMP-9免疫阳性细胞平均灰度值比较(±s)

Table 1 The expression of MMP-9 protein in each group(±s)

组别	n	6 h	24 h	48 h
HIE组	6	150.64±3.92	155.98±2.37	152.90±2.52
金纳多治疗组	6	122.30±4.71^ab	128.26±4.65^ab	125.27±2.91^ab
姜黄素治疗组	6	123.27±3.91^ab	128.99±3.85^ab	127.33±4.85^ab
综合治疗组	6	111.27±3.76^abcd	119.56±5.44^abcd	117.82±2.55^abcd
假手术组	6	107.26±3.51	95.48±2.72	109.47±4.23
t	?	13.10	22.21	14.55
P	?	0.001	0.001	0.001

表1 各组MMP-9免疫阳性细胞平均灰度值比较(±s)

Table 1 The expression of MMP-9 protein in each group(±s)

组别	n	6 h	24 h	48 h
HIE组	6	150.64±3.92	155.98±2.37	152.90±2.52
金纳多治疗组	6	122.30±4.71^ab	128.26±4.65^ab	125.27±2.91^ab
姜黄素治疗组	6	123.27±3.91^ab	128.99±3.85^ab	127.33±4.85^ab
综合治疗组	6	111.27±3.76^abcd	119.56±5.44^abcd	117.82±2.55^abcd
假手术组	6	107.26±3.51	95.48±2.72	109.47±4.23
t	?	13.10	22.21	14.55
P	?	0.001	0.001	0.001

图1 五组大鼠不同亚组脑组织MMP-9阳性细胞平均灰度值折线图

Figure 1 Five groups of brain tissue of rats with different subgroups MMP - 9 positive cell average grey value line chart

图4 金纳多治疗组HIE后6 h MMP-9的表达 (DAB×400)

Figure 4 Skinner than 6 h after treatment group HIE the expression of MMP-9 (DAB×400)

图7 假手术组HIE后24 h MMP-9的表达 (DAB×400)

Figure 7 Control HIE 24 h after the expression of MMP-9 (DAB×400)

图10 姜黄素治疗组HIE后24 h MMP-9的表达 (DAB×400)

Figure 10 24 h after curcumin treatment group HIE the expression of MMP-9 (DAB×400)

图13 HIE组HIE后48 h MMP-9的表达 (DAB×400)

Figure 13 HIE group 48 h after the expression of MMP-9 (DAB×400)

图16 综合治疗组HIE后48 h MMP-9的表达 (DAB×400)

Figure 16 Comprehensive treatment group 48 h after HIE the expression of MMP-9 (DAB×400)

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