Prediction and Analysis of B-Cell Epitope in the UL148 Gene of Human Cytomegalovirus

doi:10.3877/cma.j.issn.1673-5250.2013.04.004

Objective

To investigate the B-cell epitope in the UL148 gene of human cytomegalovirus (HCMV).

Methods

The secondary structure were analyzed by the improved self-optimized prediction method (SOPMA), Garnier-Osguthorpe-Robson(GOR), Hierarchical neutral network (HNN) Programs based on the amino acid sequence of HCMVUL148 gene. In combination with transmembrane, hydrophilicity, accessibility, flexibility and antigenicity analysis to selected the potential candidates of linear B-cell epitopes.

Results

HCMV pUL148 contains 316 amino acid residues, the relative molecular mass was 36 kD and the isoelectric point was 9.15; Variety of forecasting methods were prompted that the 14-21, 98-106, 132-139, 174-181, 191-197, 267-272, 235-240 amino acid sequence regions were main random coil or β-turn model in the secondary structure; The 1-285 amino acids was a transmembrane region and the extracellular regions was 1-285 amino acids, all of that were predicted by the TMHMM.

Conclusions

Comprehensive analysis that the 265-275, 221-229, 18-25 amino acid sequence regions were main random coil or β-turn model in the secondary structure, and consider of the average antigenicity index (AI) of epitope peptide investigation, three peptides were selected as potential candidates of linear B-cell epitopes.

Key words: human cytomegalovirus, UL148, B-cell epitope, multi-peptide immunization

图1 pUL148氨基酸序列跨膜结构预测

Figure 1 The predicted transmembrane region of pUL148

图2 pUL148氨基酸序列二级结构分析(H: α-螺旋；E：β-折叠；C：无规则卷；T：β-转角)

Figure 2 The predicted secondary structures of pUL48 amino acid sequences ( H: α-helix, E：β-sheet, C：randon coil, T：β-turn)

表1 应用不同方法预测pUL148 B细胞表位的结果

Table 1 Application of different methods to predict pUL148 B cell epitope

预测方案	预测结果
二级结构	14～21，98～106，132～139，174～181，191～197，267～272，235-240
亲水性方案	18～25，58～63，75～95，132～139，161～176，221～229，269～275
可及性方案	80～86，133～141，203～212，252～258，265～275
极性方案	22～27，58～64，75～83，132～137，161～166，168～176，206～214，221～238
柔韧性方案	15～27，29～38，43～52，70～109，113～125，130～143，155～176，183～198，203～211，220～230，236～245，253～282，284～289
抗原性方案	15～25，30～37，43～51，57～66，70～94，98～103，131～139，156～169，173～178，192～199，201～211，221～229，234～242，251～261，265～276
ABCpred服务器	74～89，259～274，205～220，80～95，157～172，167～182，240～255，225～240，123～138，112～127

表1 应用不同方法预测pUL148 B细胞表位的结果

Table 1 Application of different methods to predict pUL148 B cell epitope

预测方案	预测结果
二级结构	14～21，98～106，132～139，174～181，191～197，267～272，235-240
亲水性方案	18～25，58～63，75～95，132～139，161～176，221～229，269～275
可及性方案	80～86，133～141，203～212，252～258，265～275
极性方案	22～27，58～64，75～83，132～137，161～166，168～176，206～214，221～238
柔韧性方案	15～27，29～38，43～52，70～109，113～125，130～143，155～176，183～198，203～211，220～230，236～245，253～282，284～289
抗原性方案	15～25，30～37，43～51，57～66，70～94，98～103，131～139，156～169，173～178，192～199，201～211，221～229，234～242，251～261，265～276
ABCpred服务器	74～89，259～274，205～220，80～95，157～172，167～182，240～255，225～240，123～138，112～127

图3 pUL148亲水性Hopp & Woods(A)、极性Zimmerman(B)、表面可及性Emini(C)、柔韧性(D)、抗原性Jameson-Wolf(E)参数预测

Figure 3 The predicted by hydrophilic (A), polarity (B), the surface accessibility (C), flexibility (D), antigenic (E) of pUL148

表2 pUL148 B细胞表位抗原指数

Table 2 Antigenic index of pUL148

B细胞表位	氨基酸序列	AI
18～ 25	VNASRDYV	0.007
132～139	NDGERPQQ	0.005
221～229	ILKPRGVRH	0.044
265～275	TLSPKARLPAK	0.045

表2 pUL148 B细胞表位抗原指数

Table 2 Antigenic index of pUL148

B细胞表位	氨基酸序列	AI
18～ 25	VNASRDYV	0.007
132～139	NDGERPQQ	0.005
221～229	ILKPRGVRH	0.044
265～275	TLSPKARLPAK	0.045

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