Correlation and Differences Between Tissues and Serum Level of CA125 and CA19-9 in Endometrial Cancer

doi:10.3877/cma.j.issn.1673-5250.2013.02.013

Objective

To discuss the expression and relationship of the tissue and serum level of CA₁₂₅ and CA_19-9 in endometrial cancer.

Methods

From April 2008 to April 2012, sixty-five cases of endometrial cancer were selected into this study (endometrial cancer group). Meanwhile, thirty cases of normal endometria were included into control group and 40 cases of endometriosis (EMs) were selected as EMs group. The serum levels of CA₁₂₅ and CA_19-9 were detected by ELISA, and the histological expression level of CA₁₂₅ and CA_19-9 were detected by immunohistochemistry and Western blot. The correlation of CA₁₂₅ and CA_19-9 were analyzed. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Affiliated Lianjiang Hospital of Guangdong Medical College. Informed consent was obtained from all participates.

Results

The expression of CA₁₂₅ and CA_19-9 in endometrial cancer group were significantly higher than those in EMs group and control group (P<0.05). The level of CA₁₂₅ and CA_19-9 in EMs group were significantly higher than those of control group (P<0.05). The serum level of CA₁₂₅ and CA_19-9 were significantly related with histological expression (r=1.12, P<0.05; r=2.04, P<0.05).

Conclusions

CA₁₂₅ and CA_19-9 have high expression in endometrial cancer, and they have positive correlation between serum level and histology level, which have important diagnostic value.

Key words: endometrial cancer, CA₁₂₅, CA_19-9, correlation

表1 3组患者血清CA₁₂₅和CA_19-9水平及其阳性率比较(±s)

Table 1 Comparison of serum CA₁₂₅ and CA_19-9 levels and positive rates among three groups (±s)

组别	n	CA₁₂₅		CA_19-9
组别	n	水平(U/mL)	阳性率[n(%)]	水平(U/mL)	阳性率[n(%)]
子宫内膜癌组	65	98.40±7.71^{a b}	62(95.38)^{a b}	112.34±10.71^{a b}	63(96.92)^{a b}
EMs组	40	48.36±5.56^a	18(45.00)^a	51.91±6.62^a	21(52.50)^a
对照组	30	28.27±4.21	1 (3.33)	30.32±3.65	2 (6.67)
χ²/t		21.54	19.64	11.62	24.87

表1 3组患者血清CA₁₂₅和CA_19-9水平及其阳性率比较(±s)

Table 1 Comparison of serum CA₁₂₅ and CA_19-9 levels and positive rates among three groups (±s)

组别	n	CA₁₂₅		CA_19-9
组别	n	水平(U/mL)	阳性率[n(%)]	水平(U/mL)	阳性率[n(%)]
子宫内膜癌组	65	98.40±7.71^{a b}	62(95.38)^{a b}	112.34±10.71^{a b}	63(96.92)^{a b}
EMs组	40	48.36±5.56^a	18(45.00)^a	51.91±6.62^a	21(52.50)^a
对照组	30	28.27±4.21	1 (3.33)	30.32±3.65	2 (6.67)
χ²/t		21.54	19.64	11.62	24.87

图1 3组HE形态及CA₁₂₅和CA_19-9免疫组化染色(HE×100，免疫组化×100，A：正常子宫内膜，B：EMs，C：子宫内膜癌)

Figure 1 The observation of HE morphological and immunohistochemical staining of CA₁₂₅ and CA_19-9 in three groups (A: Normal endometrial, B: Endometriosis, C: Endometrial cancer)

表2 3组标本的免疫组化结果的CA₁₂₅和CA_19-9阳性率比较[n(%)]

Table 2 Comparison of positive rates of immunohistochemistry of CA₁₂₅ and CA_19-9 among three groups[n(%)]

组别	n	CA₁₂₅		CA_19-9
组别	n	阳性率	阴性率	阳性率	阴性率
子宫内膜癌组	65	60(92.31)^ab	5 (7.69)^{a b}	61(93.85)^ab	4 (6.15)^ab
EMs组	40	25(62.50)^a	15(37.50)^a	28(70.00)^a	12(30.00)^a
对照组	30	2 (6.67)	28(93.33)	1 (3.33)	29(96.67)
χ²		7.92	10.32	8.87	7.21

表2 3组标本的免疫组化结果的CA₁₂₅和CA_19-9阳性率比较[n(%)]

Table 2 Comparison of positive rates of immunohistochemistry of CA₁₂₅ and CA_19-9 among three groups[n(%)]

组别	n	CA₁₂₅		CA_19-9
组别	n	阳性率	阴性率	阳性率	阴性率
子宫内膜癌组	65	60(92.31)^ab	5 (7.69)^{a b}	61(93.85)^ab	4 (6.15)^ab
EMs组	40	25(62.50)^a	15(37.50)^a	28(70.00)^a	12(30.00)^a
对照组	30	2 (6.67)	28(93.33)	1 (3.33)	29(96.67)
χ²		7.92	10.32	8.87	7.21

图2 Western blot检测CA₁₂₅和CA_19-9的表达(1：子宫内膜癌组，2：EMs组，3：正常组)

Figure 2 The expression of CA₁₂₅ and CA_19-9 in the three groups detected by Western blot (A: Endometrial cancer, B: Endometriosis, C: Normal endometrial)

[1]	Pan ZZ, Bruening W, Godwin AK. Involvement of RHO GTPases and ERK in synuclein-gamma enhanced cancer cell motility[J]. Int J Oncol, 2006, 29(5):1201-1205.
[2]	Liu C, Dong B, Lu A, et al. Synuclein gamma predicts poor clinical outcome in colon cancer with normal levels of carcinoembryonic antigen[J]. BMC Cancer, 2010, 10:359.
[3]	Li J, Dowdy S, Tipton T, et al. HE4 as a biomarker for ovarian and endometrial cancer management[J]. Expert Rev Mol Diagn, 2009, 9(6):555-566.
[4]	Su L, Zhang ZT, Sha YC. Diagnostic value of combined measurement of CA₁₂₅, CA_19-9 and Doppler ultrasound in patients with ovarian tumor[J]. Anhui Med Pharmaceut J, 2010, 14(8):924-925.
[5]	McBean JH, Brumsted JR. In vitro CA₁₂₅ secretion by endometrium from women with advanced endometriosis[J]. Fertil Steril, 1993, 59(1):89-92.
[6]	Wang XY, Ye J, Feng SW, et al. Value of serum CA₁₂₅ detection for predicting recurrence and prognosis[J]. Chin J Obstet Gynecol, 2010, 45(11):813-816.
[7]	Fan YJ, Zou J, Xu H, et al. Relationships between the expression of γ-synuclein and serum CA₁₂₅ level with clinicopathological characteristics in endometrial carcionoma[J]. J Pract Obstet Gynecol, 2012, 28(3):221-223.
[8]	Waraya M, Yamashita K, Katagiri H, et al. Preoperative serum CA_19-9 and dissected peripancreatic tissue margin as determiners of long-term survival in pancreatic cancer[J]. Ann Surg Oncol, 2009, 16(5):1231-1240.
[9]	Harada T, Kubota T, Aso T. Usefulness of CA_19-9 versus CA₁₂₅ for the diagnosis of endometriosis[J]. Fertil Steril, 2002, 78(4):733-739.
[10]	Qian M, Yuan JJ, Wang XH, et al.Diagnosis and prognosis value of the serum CA₁₂₅, CA199, CEA detection in patients with epithelinl ovarian cancer[J]. China Pract Med, 2010, 5(26):53-54.

[1]	Meiyi Chen, Yiyao Li, Mengyuan Zhang, Jie Xu, Ruofan Ma, Deng Li, Jing Gu. Application research of visual self-rating system for hip joint function evaluation[J]. Chinese Journal of Joint Surgery(Electronic Edition), 2024, 18(03): 320-328.
[2]	Zhi Sun, Fangqing Zhang. Expression of serum biomarkers in patients with hip dysplasia complicated with osteoarthritis[J]. Chinese Journal of Joint Surgery(Electronic Edition), 2024, 18(01): 24-29.
[3]	Huajuan Shen, Jianbo Zhuang, Chun Liu. Correlation between Helicobacter pylori infection antibody typing and the severity of gastric mucosal inflammatory lesions and mucosal histological changes[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2024, 18(03): 156-162.
[4]	Duo Li, Zhaozhao Hao, Yanwei Chen, Yandong Nan. Correlation analysis of serum PTX3 expression and bone metastasis in non-small cell lung cancer[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2024, 17(04): 558-562.
[5]	Hongyun Liu, Cui Li, Haitang Zhang, Yuying Zhang, Chengxiang Huang. After umbilical single-arch laparoscopic technology application in endometrial cancer surgery[J]. Chinese Journal of Laparoscopic Surgery(Electronic Edition), 2024, 17(03): 182-184.
[6]	Fuxing Zhou, Yanli He, Wei Zhang. The application of laparoendoscopic single-site surgery technology in the endometrial cancer[J]. Chinese Journal of Laparoscopic Surgery(Electronic Edition), 2024, 17(01): 55-57.
[7]	Xianyong Liu, Dongmei Qin, Ruomei Zhang, Junjiao Li, Chunqin Meng, Mingxin Wu, Yuhong Wang, Xinxian Zhao, Ruilian Xv, Wenwen Hong, Ling Ma, Wei Qiu, Yu Zhou. Expression and significance of Her2/Hes1 in three pathological stages of Correa cascade in intestinal gastric cancer[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2024, 14(06): 321-327.
[8]	Sihan Liu, Jing Zhang. Relationship of 25-hydroxyvitamin D3 and the ratio of serum iron to C-reactive protein with renal function impairment in the elderly[J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2024, 13(05): 268-272.
[9]	Xiaojiu Tang, Man Hu, Bijun Xu, Ya Xiao. Study on the correlation between the severity of obesity combined with gastroesophageal reflux disease patients and their anxiety, depression and nutritional status[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2024, 14(04): 360-364.
[10]	Jian Zou, Jianhua Cong, Yue Tian, Minkun Huang, Ting Sun, Zhiqin Wang, Lihua Shen, Zhenhui Gao. Study on the correlation between pulmonary ground-glass nodules and Helicobacter pylori infection[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2024, 14(03): 258-262.
[11]	Fei Yang, Tengfei Liu, Zhijun Zhao, Ruicong Li, Jie Zhang, Yan Liu, Zhen Zhao. Association between serum vitamin levels and differentiated thyroid cancer[J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(07): 633-640.
[12]	Lin Xu. Progress in understanding of relationship between thyroid hormones and related diseases and sex hormones[J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(03): 315-318.
[13]	Di Wu, Zhifeng Yan, Mingxia Li, Yuanguang Meng. Immunotherapy for advanced endometrial cancer: an exploratory study[J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(03): 231-237.
[14]	Wenjuan Li, Fan Yang, Anqi Zhang, Xiaoqing Yin, Yuanyuan Li, Fangfan Zheng. Correlation between the imaging manifestation of spina bifida occulta and clinical symptoms and curative effect in children with nocturnal enuresis[J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2024, 12(04): 356-361.
[15]	Zhichao Liu, Fengyun Hu, Chunli Wen. Risk factors and geographical correlation of stroke in Shanxi Province[J]. Chinese Journal of Cerebrovascular Diseases(Electronic Edition), 2024, 18(05): 424-433.

Viewed

Full text

Abstract

Please choose a citation manager

Content to export