Impact of Tanshinone ⅡA on Human Umbilical Vein Endothelial Cells Injured by Serum of Preeclampsia

doi:10.3877/cma.j.issn.1673-5250.2012.02.008

Objective

To explore the impact of tanshinone ⅡA(Tan ⅡA)on the proliferation of human umbilical vein endothelial cells(HUVEC) effected by serum of preeclampsia.

Methods

From December 2010 to March 2011, a total of 60 serum samples which took from 20 patients with preeclampsia(3 serum samples each patient) were divided into preeclampsia group(n=20), 1.0 mg/L TanⅡA group (n=20) and 2.0 mg/L TanⅡA group(n=20) according to the usage of TanⅡA. Meanwhile, 20 serum samples taken from 20 normal late pregnancy women were chose in the same hospital for treatment (1 serum sample each patient) as blank control group.The study protocol was approved by the Ethical Review Board of Investigation in Human Being of First Hospital of Harbin Medical University.Informed consent was obtained from each patients. There was no significant difference in age and gestational age between preeclampsia patients and normal late pregnancy women (P>0.05). The morphologic changes of HUVEC were observed under inverted microscope. Cell counting kit-8(CCK-8) assay was applied to detect HUVEC optical density (OD) value (HUVEC vitality) in each group, and analyzed that statistically.

Results

Under inverted microscope, cells of preeclampsia group was injured, showed that cell was sparse, cytoplasm and nucleus became blurred, and dark particles was found in cytoplasm. For 1.0 mg/L TanⅡA group and 2.0 mg/L TanⅡA group, The morphologic changes of HUVEC were polygonal, oval, closely arranged, and clear cell boundaries under the microscope. OD value in four serum samples were cultured at 24 h and 48 h, there was significant difference between preeclampsia group and blank control group (P<0.05); OD value in both 1.0 mg/L TanⅡA treatment group and 2.0 mg/L TanⅡA group were obviously higher than those in preeclampsia group, respectively (P<0.05). There also had significant difference between 1.0 mg/L TanⅡA treatment group with 2.0 mg/L TanⅡA treatment group(P<0.05).

Conclusions

The stimulation of preeclampsia serum might cause the decrease viability of endothelial HUVEC. Tan ⅡA can protect HUVEC from damage induced by preeclampsia serum.

Key words: preeclampsia, tanshinone ⅡA, human umbilical vein endothelial cells, cell counting kit-8

图1 倒置显微镜下各组细胞形态(A：空白对照组；B：子痫前期组；C：Tan ⅡA处理组)(×200)

Figure 1 Cell morphous by inverted microscope in each group(A：blank group；B：eclampsism group；C：Tan ⅡA group)(×200)

表1 4组继续培养24 h和48 h所测OD值比较(±s)

Table 1 Comparison of OD value of HUVEC after 24 h and 48 h among four groups(±s)

组别	24 h	48 h
子痫前期组	0.517±0.060	0.564±0.060
1.0 mg/L TanⅡA处理组	0.678±0.040	0.761±0.060
2.0 mg/L TanⅡA处理组	0.750±0.050	0.841±0.040
空白对照组	0.845±0.040	0.944±0.040

表1 4组继续培养24 h和48 h所测OD值比较(±s)

Table 1 Comparison of OD value of HUVEC after 24 h and 48 h among four groups(±s)

组别	24 h	48 h
子痫前期组	0.517±0.060	0.564±0.060
1.0 mg/L TanⅡA处理组	0.678±0.040	0.761±0.060
2.0 mg/L TanⅡA处理组	0.750±0.050	0.841±0.040
空白对照组	0.845±0.040	0.944±0.040

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